PMID- 28100035
OWN - NLM
STAT- MEDLINE
DCOM- 20180122
LR  - 20181202
IS  - 1549-4918 (Electronic)
IS  - 1066-5099 (Linking)
VI  - 35
IP  - 5
DP  - 2017 May
TI  - Stromal Cells Act as Guardians for Endothelial Progenitors by Reducing Their 
      Immunogenicity After Co-Transplantation.
PG  - 1233-1245
LID - 10.1002/stem.2573 [doi]
AB  - Regeneration of injured tissues requires effective therapeutic strategies 
      supporting vasculogenesis. The lack of instantly available autologous cell 
      sources and immunogenicity of allogeneic endothelial (progenitor) cells limits 
      clinical progress. Based on the immunosuppressive potency of mesenchymal 
      stem/progenitor cells (MSCs), we investigated whether crosstalk between 
      endothelial colony-forming progenitor cells (ECFCs) and MSCs during 
      vasculogenesis could lower allogeneic T cell responses against ECFCs allowing 
      long-term engraftment in vivo. Immunodeficient mice received subcutaneous grafts 
      containing human ECFCs alone, or pairs of human ECFCs/MSCs from the same 
      umbilical cord (UC) to study vasculogenesis in the presence of human leukocyte 
      antigen (HLA)-mismatched human peripheral blood mononuclear cells (PBMCs). In 
      vitro, cell surface marker changes due to interferon gamma (IFNgamma) stimulation 
      during ECFC/MSC coculture were determined and further effects on allostimulated T 
      cell proliferation and cytotoxic lysis were measured. IFNgamma-induced HLA-DR 
      expression on ECFCs and MSCs, but both cell types had significantly less HLA-DR 
      in cocultures. ECFC-induced T cell proliferation was abolished after MSC 
      coculture as a result of HLA-DR downregulation and indolamin-2,3-dioxygenase 
      activation. Additionally, allospecific CD8(+) T cell-mediated lysis of ECFCs was 
      reduced in cocultures. ECFC/MSC coapplication in immunodeficient mice not only 
      promoted the generation of improved blood vessel architecture after 6 weeks, but 
      also reduced intragraft immune cell infiltration and endothelial HLA-DR 
      expression following PBMC reconstitution. Crosstalk between UC-derived ECFCs and 
      MSCs after combined transplantation can lower the risk of ECFC rejection, thus 
      enabling their coapplication for therapeutic vasculogenesis. Stem Cells 
      2017;35:1233-1245.
CI  - (c) 2017 AlphaMed Press.
FAU - Souidi, Naima
AU  - Souidi N
AD  - Institute of Medical Immunology, Charite Universitatsmedizin Berlin, Campus 
      Virchow-Klinikum, Berlin, Germany.
AD  - Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Charite 
      Universitatsmedizin Berlin, Campus Virchow-Klinikum, Berlin, Germany.
FAU - Stolk, Meaghan
AU  - Stolk M
AD  - Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Charite 
      Universitatsmedizin Berlin, Campus Virchow-Klinikum, Berlin, Germany.
FAU - Rudeck, Juliane
AU  - Rudeck J
AD  - Institute of Medical Immunology, Charite Universitatsmedizin Berlin, Campus 
      Virchow-Klinikum, Berlin, Germany.
FAU - Strunk, Dirk
AU  - Strunk D
AD  - Institute of Experimental and Clinical Cell Therapy, Paracelsus Medical 
      University, Salzburg, Austria.
AD  - Spinal Cord & Tissue Regeneration Center, Paracelsus Medical University, 
      Salzburg, Austria.
FAU - Schallmoser, Katharina
AU  - Schallmoser K
AD  - Spinal Cord & Tissue Regeneration Center, Paracelsus Medical University, 
      Salzburg, Austria.
AD  - Department for Blood Group Serology and Transfusion Medicine, Paracelsus Medical 
      University, Salzburg, Austria.
FAU - Volk, Hans-Dieter
AU  - Volk HD
AD  - Institute of Medical Immunology, Charite Universitatsmedizin Berlin, Campus 
      Virchow-Klinikum, Berlin, Germany.
AD  - Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Charite 
      Universitatsmedizin Berlin, Campus Virchow-Klinikum, Berlin, Germany.
FAU - Seifert, Martina
AU  - Seifert M
AD  - Institute of Medical Immunology, Charite Universitatsmedizin Berlin, Campus 
      Virchow-Klinikum, Berlin, Germany.
AD  - Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Charite 
      Universitatsmedizin Berlin, Campus Virchow-Klinikum, Berlin, Germany.
LA  - eng
PT  - Journal Article
DEP - 20170223
PL  - England
TA  - Stem Cells
JT  - Stem cells (Dayton, Ohio)
JID - 9304532
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Indoleamine-Pyrrole 2,3,-Dioxygenase)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - CD4-Positive T-Lymphocytes/cytology/drug effects
MH  - Cell Proliferation/drug effects
MH  - Coculture Techniques
MH  - Colony-Forming Units Assay
MH  - Cytotoxicity, Immunologic/drug effects
MH  - Down-Regulation/drug effects
MH  - Endothelial Cells/cytology/drug effects/*immunology/*transplantation
MH  - Female
MH  - HLA-DR Antigens/metabolism
MH  - Humans
MH  - Indoleamine-Pyrrole 2,3,-Dioxygenase/metabolism
MH  - Inflammation/pathology
MH  - Interferon-gamma/pharmacology
MH  - Male
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/*cytology
MH  - Mice
MH  - Neovascularization, Physiologic/drug effects
MH  - Stromal Cells/cytology/drug effects/transplantation
OTO - NOTNLM
OT  - Cell transplantation
OT  - Endothelial progenitor cells
OT  - Histocompatibility antigens
OT  - Immunomodulation
OT  - Neovascularization
OT  - Stromal cells
EDAT- 2017/01/19 06:00
MHDA- 2018/01/23 06:00
CRDT- 2017/01/19 06:00
PHST- 2016/07/29 00:00 [received]
PHST- 2016/12/07 00:00 [revised]
PHST- 2016/12/18 00:00 [accepted]
PHST- 2017/01/19 06:00 [pubmed]
PHST- 2018/01/23 06:00 [medline]
PHST- 2017/01/19 06:00 [entrez]
AID - 10.1002/stem.2573 [doi]
PST - ppublish
SO  - Stem Cells. 2017 May;35(5):1233-1245. doi: 10.1002/stem.2573. Epub 2017 Feb 23.