PMID- 28102038
OWN - NLM
STAT- MEDLINE
DCOM- 20180607
LR  - 20180821
IS  - 2059-2310 (Electronic)
IS  - 2059-2302 (Linking)
VI  - 89
IP  - 2
DP  - 2017 Feb
TI  - EDTA is superior to DTT treatment for overcoming the prozone effect in HLA 
      antibody testing.
PG  - 82-89
LID - 10.1111/tan.12950 [doi]
AB  - A limitation of solid-phase human leukocyte antigen (HLA) antibody assays is the 
      falsely low/negative result of samples with high-titer antibodies, a phenomenon 
      known as the prozone effect. Here we compared the efficacy of 
      ethylenediaminetetraacetic acid (EDTA) and dithiothreitol (DTT) treatment of 
      serum samples in overcoming the prozone effect. A total of 21 serum samples were 
      treated with either EDTA or DTT before HLA single antigen bead assay. The 
      efficacy of prozone effect reversal, compared with untreated samples, was 
      examined on fourfold, serially diluted samples, from neat to 1:256, using PBS as 
      diluent. EDTA reversed the prozone effect in all tested samples, with an 
      efficiency of greater than 84%, estimated by the ratio of undiluted sample mean 
      fluorescence intensity (MFI) to peak MFI, for any given dilution. In contrast, 
      the efficiency of DTT treatment was as low as 47%. These results show superior 
      prozone effect reversal with EDTA treatment, compared with DTT.
CI  - (c) 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Wang, J
AU  - Wang J
AD  - Department of Pathology, University of Chicago Medicine, Chicago, Illinois.
FAU - Meade, J R
AU  - Meade JR
AD  - Department of Pathology, University of Chicago Medicine, Chicago, Illinois.
FAU - Brown, N K
AU  - Brown NK
AUID- ORCID: 0000-0002-0046-2315
AD  - Department of Pathology, University of Chicago Medicine, Chicago, Illinois.
FAU - Weidner, J G
AU  - Weidner JG
AD  - Department of Pathology, University of Chicago Medicine, Chicago, Illinois.
FAU - Marino, S R
AU  - Marino SR
AD  - Department of Pathology, University of Chicago Medicine, Chicago, Illinois.
LA  - eng
PT  - Journal Article
PL  - England
TA  - HLA
JT  - HLA
JID - 101675570
RN  - 0 (Antibodies)
RN  - 0 (HLA Antigens)
RN  - 9G34HU7RV0 (Edetic Acid)
RN  - T8ID5YZU6Y (Dithiothreitol)
SB  - IM
MH  - Antibodies/chemistry
MH  - Dithiothreitol/chemistry
MH  - Edetic Acid/*chemistry
MH  - False Negative Reactions
MH  - HLA Antigens/*blood/classification/genetics/immunology
MH  - Histocompatibility Testing/methods/*standards
MH  - Humans
MH  - Immunoassay/methods/*standards
OTO - NOTNLM
OT  - EDTA
OT  - HLA antigens
OT  - dithiothreitol
EDAT- 2017/01/20 06:00
MHDA- 2018/06/08 06:00
CRDT- 2017/01/20 06:00
PHST- 2016/08/18 00:00 [received]
PHST- 2016/12/01 00:00 [revised]
PHST- 2016/12/06 00:00 [accepted]
PHST- 2017/01/20 06:00 [entrez]
PHST- 2017/01/20 06:00 [pubmed]
PHST- 2018/06/08 06:00 [medline]
AID - 10.1111/tan.12950 [doi]
PST - ppublish
SO  - HLA. 2017 Feb;89(2):82-89. doi: 10.1111/tan.12950.