PMID- 28102609
OWN - NLM
STAT- MEDLINE
DCOM- 20180221
LR  - 20181202
IS  - 1399-3089 (Electronic)
IS  - 0908-665X (Linking)
VI  - 24
IP  - 1
DP  - 2017 Jan
TI  - Immunogenicity of hepatic differentiated human umbilical cord mesenchymal stem 
      cells promoted by porcine decellularized liver scaffolds.
LID - 10.1111/xen.12287 [doi]
AB  - Cell-based approaches, including hepatocyte transplantation and tissue-engineered 
      livers, offer promising alternatives and are expected to help support patients 
      with liver diseases until liver transplantation or recovery via regeneration of 
      the damaged liver. However, the success of cell therapies remains dependent on 
      how well the cells are accepted after transplantation and is directly related to 
      their degree of immunogenicity. In this study, hepatic differentiation of human 
      umbilical cord mesenchymal stem cells (hUC-MSCs) was induced in the traditional 
      monolayer (2D) culture and newly established three-dimensional (3D) aggregation 
      culture with the porcine decellularized liver scaffold (DLS) system (3D-DLS). We 
      investigated the immunogenicity of these hepatocyte-like cells in vitro. We found 
      that monolayer hepatic differentiated hUC-MSCs expressed higher levels of human 
      leukocyte antigen-DR (HLA-DR) (P<.05) and lost the ability to inhibit lymphocyte 
      proliferation (P<.05), in association with a lower level of prostaglandin E(2) 
      (PGE(2) ) (P<.05) and a higher level of interferon-gamma (IFN-gamma) (P<.05) secretion, 
      compared to undifferentiated hUC-MSCs. The hepatocyte-like cells differentiated 
      in the 3D-DLS system did not show an elevation of MHC-II (P>.05), or cause 
      obvious lymphocytes proliferation, and demonstrated more PGE(2) (P<.05) and less 
      IFN-gamma (P<.05) secretion. Hepatocyte-like cells in the 3D-DLS system presented a 
      lower immunogenic phenotype than the 2D culture in vitro. Hepatocyte-like cells 
      in 3D-DLS system also performed a higher immunosuppressive capacity than 2D 
      culture.
CI  - (c) 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Li, Yi
AU  - Li Y
AD  - Laboratory of Pathology, West China Hospital, Sichuan University, Chengdu, 
      Sichuan, China.
AD  - Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West 
      China Hospital, Sichuan University, Chengdu, China.
AD  - Division of Transplant Surgery, Department of Surgery, Mayo Clinic, Rochester, 
      MN, USA.
FAU - Wu, Qiong
AU  - Wu Q
AD  - Laboratory of Pathology, West China Hospital, Sichuan University, Chengdu, 
      Sichuan, China.
AD  - Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West 
      China Hospital, Sichuan University, Chengdu, China.
FAU - Wang, Yujia
AU  - Wang Y
AD  - Laboratory of Pathology, West China Hospital, Sichuan University, Chengdu, 
      Sichuan, China.
AD  - Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West 
      China Hospital, Sichuan University, Chengdu, China.
AD  - Division of Transplant Surgery, Department of Surgery, Mayo Clinic, Rochester, 
      MN, USA.
FAU - Li, Li
AU  - Li L
AD  - Laboratory of Pathology, West China Hospital, Sichuan University, Chengdu, 
      Sichuan, China.
AD  - Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West 
      China Hospital, Sichuan University, Chengdu, China.
FAU - Chen, Fei
AU  - Chen F
AD  - Laboratory of Pathology, West China Hospital, Sichuan University, Chengdu, 
      Sichuan, China.
AD  - Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West 
      China Hospital, Sichuan University, Chengdu, China.
FAU - Shi, Yujun
AU  - Shi Y
AD  - Laboratory of Pathology, West China Hospital, Sichuan University, Chengdu, 
      Sichuan, China.
AD  - Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West 
      China Hospital, Sichuan University, Chengdu, China.
FAU - Bu, Hong
AU  - Bu H
AD  - Laboratory of Pathology, West China Hospital, Sichuan University, Chengdu, 
      Sichuan, China.
AD  - Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West 
      China Hospital, Sichuan University, Chengdu, China.
AD  - Department of Pathology, West China Hospital, Sichuan University, Chengdu, 
      Sichuan, China.
FAU - Bao, Ji
AU  - Bao J
AUID- ORCID: 0000-0001-8413-3270
AD  - Laboratory of Pathology, West China Hospital, Sichuan University, Chengdu, 
      Sichuan, China.
AD  - Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West 
      China Hospital, Sichuan University, Chengdu, China.
LA  - eng
PT  - Journal Article
DEP - 20170119
PL  - Denmark
TA  - Xenotransplantation
JT  - Xenotransplantation
JID - 9438793
SB  - IM
MH  - Animals
MH  - Cell Differentiation/physiology
MH  - Cells, Cultured
MH  - Hepatocytes/*cytology
MH  - Humans
MH  - Liver/*cytology
MH  - Mesenchymal Stem Cell Transplantation/methods
MH  - Mesenchymal Stem Cells/*cytology
MH  - Swine
MH  - Tissue Engineering/methods
MH  - *Transplantation, Heterologous
MH  - Umbilical Cord/*cytology
OTO - NOTNLM
OT  - decellularized liver scaffold
OT  - hepatic differentiation
OT  - immunogenicity
OT  - mesenchymal stem cells
OT  - three dimension
EDAT- 2017/01/20 06:00
MHDA- 2018/02/22 06:00
CRDT- 2017/01/20 06:00
PHST- 2016/08/17 00:00 [received]
PHST- 2016/12/09 00:00 [revised]
PHST- 2016/12/13 00:00 [accepted]
PHST- 2017/01/20 06:00 [pubmed]
PHST- 2018/02/22 06:00 [medline]
PHST- 2017/01/20 06:00 [entrez]
AID - 10.1111/xen.12287 [doi]
PST - ppublish
SO  - Xenotransplantation. 2017 Jan;24(1). doi: 10.1111/xen.12287. Epub 2017 Jan 19.