PMID- 28104015
OWN - NLM
STAT- MEDLINE
DCOM- 20170719
LR  - 20181202
IS  - 1673-0860 (Print)
IS  - 1673-0860 (Linking)
VI  - 52
IP  - 1
DP  - 2017 Jan 7
TI  - [Establishment and characterization of a laryngeal squamous cell carcinoma cell 
      line].
PG  - 44-48
LID - 10.3760/cma.j.issn.1673-0860.2017.01.008 [doi]
AB  - Objective: To establish a laryngeal squamous cell carcinoma (LSCC) cell line 
      through primary cell culture and observe its biological characteristics. Methods: 
      Tissue block culture method was used for primary cell culture. After LSCC cells 
      passed 25 times in vitro, the morphology of cells was observed, keratin was 
      stained histochemically, cell cycle was tested by PI-FACS, and the specie of 
      cells was detected by PCR and short tandem repeat(STR) typing. Results: This 
      newly established LSCC cell line was named as TR-LCC-1, most of the cancer cells 
      were polygonal shape, like the cobblestone, loss of contact inhibition and with 
      overlapping growth. Cell size was large and cell pleomorphism was very obvious. 
      Cytokeratin staining was positive. After 6 months of continuous culture in vitro, 
      the TR-LCC-1 cells passed more than 30 times, and cell doubling time was 201.2h. 
      Cell cycle assay indicated that G1 phase accounted for 51.71%, S phase was 
      44.56%, and G2 phase was 2.28%. Mycoplasma test showed no mycoplasma 
      contamination. Cell species identification identified TR-LCC-1 was human-derived 
      cells. STR detection showed P26 and P6 were same, and they were different from 
      the STR typing of disclosed cells. Conclusion: The establish ment of the new 
      laryngeal squamous carcinoma cell line TR-LCC-1 can be helpful to the research 
      for laryngeal squamous cell cancer.
FAU - Wang, R
AU  - Wang R
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren 
      Hospital, Capital Medical University, Beijing 100730, China; Key Laboratory of 
      Otorhinolaryngology, Ministry of Education, Beijing 100005, China.
FAU - Bian, X C
AU  - Bian XC
AD  - Institute of Basic Medical Science, Chinese Academy of Medical Sciences, Beijing 
      100005, China.
FAU - Liu, Y Q
AU  - Liu YQ
AD  - Institute of Basic Medical Science, Chinese Academy of Medical Sciences, Beijing 
      100005, China.
FAU - Fang, J G
AU  - Fang JG
AD  - Beijing Key Laboratory of Head and Neck Molecular Diagnostic Pathology, Beijing 
      100730, China; Key Laboratory of Otorhinolaryngology, Ministry of Education, 
      Beijing 100005, China; Department of Otorhinolaryngology Head and Neck Surgery, 
      Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China.
FAU - Wang, H
AU  - Wang H
AD  - Key Laboratory of Otorhinolaryngology, Ministry of Education, Beijing 100005, 
      China.
FAU - Liu, H G
AU  - Liu HG
AD  - Beijing Key Laboratory of Head and Neck Molecular Diagnostic Pathology, Beijing 
      100730, China.
FAU - Lian, M
AU  - Lian M
AD  - Key Laboratory of Otorhinolaryngology, Ministry of Education, Beijing 100005, 
      China; Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren 
      Hospital, Capital Medical University, Beijing 100730, China.
FAU - Ma, H Z
AU  - Ma HZ
AD  - Key Laboratory of Otorhinolaryngology, Ministry of Education, Beijing 100005, 
      China; Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren 
      Hospital, Capital Medical University, Beijing 100730, China.
FAU - Feng, L
AU  - Feng L
AD  - Key Laboratory of Otorhinolaryngology, Ministry of Education, Beijing 100005, 
      China; Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren 
      Hospital, Capital Medical University, Beijing 100730, China.
FAU - Wang, H Z
AU  - Wang HZ
AD  - Key Laboratory of Otorhinolaryngology, Ministry of Education, Beijing 100005, 
      China; Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren 
      Hospital, Capital Medical University, Beijing 100730, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
JT  - Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of 
      otorhinolaryngology head and neck surgery
JID - 101247574
RN  - 68238-35-7 (Keratins)
SB  - IM
MH  - Carcinoma, Squamous Cell/chemistry/*pathology
MH  - Cell Cycle
MH  - Cell Line, Tumor/chemistry/*pathology
MH  - Humans
MH  - Keratins/analysis
MH  - Laryngeal Neoplasms/chemistry/*pathology
OTO - NOTNLM
OT  - Carcinoma, squamous cell
OT  - Cell line
OT  - Laryngeal neoplasms
OT  - Tumor cells, cultured
EDAT- 2017/01/21 06:00
MHDA- 2017/07/20 06:00
CRDT- 2017/01/21 06:00
PHST- 2017/01/21 06:00 [entrez]
PHST- 2017/01/21 06:00 [pubmed]
PHST- 2017/07/20 06:00 [medline]
AID - 10.3760/cma.j.issn.1673-0860.2017.01.008 [doi]
PST - ppublish
SO  - Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2017 Jan 7;52(1):44-48. doi: 
      10.3760/cma.j.issn.1673-0860.2017.01.008.