PMID- 28105653
OWN - NLM
STAT- MEDLINE
DCOM- 20170718
LR  - 20181113
IS  - 1521-4141 (Electronic)
IS  - 0014-2980 (Print)
IS  - 0014-2980 (Linking)
VI  - 47
IP  - 3
DP  - 2017 Mar
TI  - Properdin and factor H production by human dendritic cells modulates their T-cell 
      stimulatory capacity and is regulated by IFN-gamma.
PG  - 470-480
LID - 10.1002/eji.201646703 [doi]
AB  - Dendritic cells (DCs) and complement are both key members of the innate and 
      adaptive immune response. Recent experimental mouse models have shown that 
      production of alternative pathway (AP) components by DCs strongly affects their 
      ability to activate and regulate T-cell responses. In this study we investigated 
      the production and regulation of properdin (fP) and factor H (fH) both integral 
      regulators of the AP, by DCs and tolerogenic DCs (tolDCs). Both fP and fH were 
      produced by DCs, with significantly higher levels of both AP components produced 
      by tolDCs. Upon activation with IFN-gamma both cells increased fH production, while 
      simultaneously decreasing production of fP. IL-27, a member of the IL-12 family, 
      increased fH, but production of fP remained unaffected. The functional capacity 
      of fP and fH produced by DCs and tolDCs was confirmed by their ability to bind 
      C3b. Inhibition of fH production by DCs resulted in a greater ability to induce 
      allogenic CD4(+) T-cell proliferation. In contrast, inhibition of fP production 
      led to a significantly reduced allostimulatory capacity. In summary, this study 
      shows that production of fP and fH by DCs, differentially regulates their 
      immunogenicity, and that the local cytokine environment can profoundly affect the 
      production of fP and fH.
CI  - (c) 2017 The Authors. European Journal of Immunology published by WILEY-VCH Verlag 
      GmbH & Co. KGaA, Weinheim.
FAU - Dixon, Karen O
AU  - Dixon KO
AD  - Department of Nephrology, Leiden University Medical Center, Leiden, The 
      Netherlands.
AD  - Evergrande Center for Immunologic Diseases at Harvard Medical School and Brigham 
      and Women's Hospital, Boston, MA, USA.
FAU - O'Flynn, Joseph
AU  - O'Flynn J
AD  - Department of Nephrology, Leiden University Medical Center, Leiden, The 
      Netherlands.
AD  - Program in Cellular and Molecular Medicine, Boston Children's Hospital, Harvard 
      Medical School, Boston, MA, USA.
FAU - Klar-Mohamad, Ngaisah
AU  - Klar-Mohamad N
AD  - Department of Nephrology, Leiden University Medical Center, Leiden, The 
      Netherlands.
FAU - Daha, Mohamed R
AU  - Daha MR
AD  - Department of Nephrology, Leiden University Medical Center, Leiden, The 
      Netherlands.
FAU - van Kooten, Cees
AU  - van Kooten C
AD  - Department of Nephrology, Leiden University Medical Center, Leiden, The 
      Netherlands.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Eur J Immunol
JT  - European journal of immunology
JID - 1273201
RN  - 0 (Interleukin-27)
RN  - 0 (Isoantigens)
RN  - 0 (RNA, Small Interfering)
RN  - 11016-39-0 (Properdin)
RN  - 80295-43-8 (Complement C3b)
RN  - 80295-65-4 (Complement Factor H)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - CD4-Positive T-Lymphocytes/*immunology
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Complement C3b/metabolism
MH  - Complement Factor H/genetics/*metabolism
MH  - *Complement Pathway, Alternative
MH  - Dendritic Cells/*physiology
MH  - Humans
MH  - Immune Tolerance
MH  - Interferon-gamma/metabolism
MH  - Interleukin-27/metabolism
MH  - Isoantigens/immunology
MH  - Lymphocyte Activation
MH  - Properdin/genetics/*metabolism
MH  - RNA, Small Interfering/genetics
PMC - PMC5363362
OTO - NOTNLM
OT  - Complement
OT  - Cytokines
OT  - Factor H
OT  - IFN-gamma
OT  - IL-27
OT  - Properdin
OT  - Tolerogenic dendritic cells
EDAT- 2017/01/21 06:00
MHDA- 2017/07/19 06:00
PMCR- 2017/03/23
CRDT- 2017/01/21 06:00
PHST- 2016/08/29 00:00 [received]
PHST- 2016/12/16 00:00 [revised]
PHST- 2017/01/17 00:00 [accepted]
PHST- 2017/01/21 06:00 [pubmed]
PHST- 2017/07/19 06:00 [medline]
PHST- 2017/01/21 06:00 [entrez]
PHST- 2017/03/23 00:00 [pmc-release]
AID - EJI3845 [pii]
AID - 10.1002/eji.201646703 [doi]
PST - ppublish
SO  - Eur J Immunol. 2017 Mar;47(3):470-480. doi: 10.1002/eji.201646703.