PMID- 28109303
OWN - NLM
STAT- MEDLINE
DCOM- 20170810
LR  - 20240324
IS  - 0717-6287 (Electronic)
IS  - 0716-9760 (Print)
IS  - 0716-9760 (Linking)
VI  - 50
IP  - 1
DP  - 2017 Jan 21
TI  - Uptake of DNA by cancer cells without a transfection reagent.
PG  - 2
LID - 10.1186/s40659-017-0107-x [doi]
LID - 2
AB  - BACKGROUND: Cancer cells exhibit elevated levels of glucose uptake and may obtain 
      pre-formed, diet-derived fatty acids from the bloodstream to boost their rapid 
      growth; they may also use nucleic acid from their microenvironment. The study of 
      processing nucleic acid by cancer cells will help improve the understanding of 
      the metabolism of cancer. DNA is commonly packaged into a viral or lipid particle 
      to be transferred into cells; this process is called transfection in laboratory. 
      Cancer cells are known for having gene mutations and the evolving ability of 
      endocytosis. Their uptake of DNAs might be different from normal cells; they may 
      take in DNAs directly from the environment. In this report, we studied the uptake 
      of DNAs in cancer cells without a transfection reagent. METHODS: A group of DNA 
      fragments were prepared with PCR and labeled with isotope phosphorous-32 to test 
      their uptake by Huh 7 (liver cancer) and THLE3 (normal liver cells) after 
      incubation overnight by counting radioactivity of the cells' genomic DNA. 
      Multiple cell lines including breast cancer and lung cancer were tested with the 
      same method. DNA molecules were also labeled with fluorescence to test the 
      location in the cells using a kit of "label it fluorescence in situ hybridization 
      (FISH)" from Mirus (USA). RESULTS: The data demonstrated that hepatocellular 
      carcinoma cells possess the ability to take in large DNA fragments directly 
      without a transfection reagent whereas normal liver cells cannot. Huh7 and 
      MDA-MB231 cells displayed a significantly higher Rhodamine density in the 
      cytoplasmic phagosomes and this suggests that the mechanism of uptake of large 
      DNA by cancer cells is likely endocytosis. The efficacy of uptake is related to 
      the DNA's size. Some cell lines of lung cancer and breast cancer also showed 
      similar uptake of DNA. CONCLUSIONS: In the present study, we have revealed the 
      evidence that some cancer cells, but not nontumorigenic cells, can take DNA 
      fragments directly from the environment without the aid of the transfecting 
      reagent.
FAU - Kong, Yanping
AU  - Kong Y
AD  - Department of Endocrinology, Dartmouth-Hitchcock Manchester, 100 Hitchcock Way, 
      Manchester, New Hampshire, 03104, USA. yanping.kong@hitchcock.org.
FAU - Zhang, Xianbo
AU  - Zhang X
AD  - Department of Surgery/Oncology, First Hospital of Shijiazhuang, 36 Fanxi Road, 
      Shijiazhuang, 050011, Hebei, China.
FAU - Zhao, Yongliang
AU  - Zhao Y
AD  - Laboratory of Disease Genomics and Individualized Medicine, Beijing Institute of 
      Genomics, Chinese Academy of Sciences, 1 Beichen West Road, Beijing, 10029, 
      China.
FAU - Xue, Yanfang
AU  - Xue Y
AD  - Department of Pharmacology, Hebei Medical University, 361 Zhongshan E Rd, 
      Shijiazhuang, 050056, Hebei, China.
FAU - Zhang, Ye
AU  - Zhang Y
AD  - National Laboratory of Medical Molecular Biology, Department of Biochemistry and 
      Molecular Biology, Chinese Academy of Medical Sciences & Peking Union Medical 
      College, Dongdan Santiao, Beijing, 100005, China.
LA  - eng
PT  - Journal Article
DEP - 20170121
PL  - England
TA  - Biol Res
JT  - Biological research
JID - 9308271
RN  - 0 (FuGene)
RN  - 0 (Lipids)
RN  - 0 (alpha-Fetoproteins)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Breast Neoplasms/genetics/pathology
MH  - Cell Line
MH  - Cell Line, Tumor
MH  - DNA/*metabolism
MH  - DNA Fragmentation
MH  - Endocytosis/genetics
MH  - Female
MH  - Genomics
MH  - Hepatocytes/metabolism
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lipids/pharmacology
MH  - Liver Neoplasms/genetics/pathology
MH  - Lung Neoplasms/genetics/pathology
MH  - Neoplasms/*genetics/pathology
MH  - Polymerase Chain Reaction
MH  - *Transfection
MH  - alpha-Fetoproteins/metabolism
PMC - PMC5251232
OTO - NOTNLM
OT  - Cancer cells
OT  - DNA uptake
OT  - Endocytosis
EDAT- 2017/01/23 06:00
MHDA- 2017/08/11 06:00
PMCR- 2017/01/21
CRDT- 2017/01/23 06:00
PHST- 2016/09/02 00:00 [received]
PHST- 2017/01/14 00:00 [accepted]
PHST- 2017/01/23 06:00 [entrez]
PHST- 2017/01/23 06:00 [pubmed]
PHST- 2017/08/11 06:00 [medline]
PHST- 2017/01/21 00:00 [pmc-release]
AID - 10.1186/s40659-017-0107-x [pii]
AID - 107 [pii]
AID - 10.1186/s40659-017-0107-x [doi]
PST - epublish
SO  - Biol Res. 2017 Jan 21;50(1):2. doi: 10.1186/s40659-017-0107-x.