PMID- 28110785
OWN - NLM
STAT- MEDLINE
DCOM- 20170227
LR  - 20170227
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 199
DP  - 2017 Feb
TI  - Genetic diversity of ORF 4-6 of type 1 porcine reproductive and respiratory 
      syndrome virus in naturally infected pigs.
PG  - 54-61
LID - S0378-1135(16)30831-8 [pii]
LID - 10.1016/j.vetmic.2016.12.026 [doi]
AB  - Genotype 1 porcine reproductive and respiratory syndrome virus (PRRSV) has been 
      highly prevalent throughout Korea since the virus was first detected in 2005. 
      However, genetic analyses of genotype 1 PRRSV in Korea have been limited to ORF5 
      and/or ORF7. In the present study, we determined 10 representative sequence 
      covering ORF4 to ORF6 and each individual ORFs of genotype 1 PRRSV in Korea, and 
      performed molecular analyses. The most variable gene among the individual ORFs of 
      field strains was ORF4, and this gene exhibited only 74.5-87.3% sequence homology 
      compared with strains reported elsewhere. However, the strains showed analogous 
      sequence arrangements with each other. In the phylogenetic analysis, the 
      sequences of Korean field strains formed a distinct cluster with some Austrian 
      and German strains compared to genotype 1 PRRSV strains available in GenBank. In 
      the amino acid analysis, the putative antigenic region of GP4 was highly 
      variable, whereas the predicted epitope regions of ORF5 and ORF6 were relatively 
      conserved. The hydropathy plots of GP4 showed a highly variable pattern in the 
      antigenic region. The non-synonymous and synonymous substitution analysis 
      suggested that ORF4 presumably had more immunogenic pressure compare with the 
      other ORFs. According to these findings, genotype 1 PRRSV in Korea has been 
      diversified and indigenized in Korea, and these strains might have multifarious 
      immunological and genetic properties. This study provides novel insights into 
      genotype 1 PRRSV in a geographically remote area and contributes to the 
      information for further research on the evolution of type 1 PRRSV in the Korean 
      peninsula.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Lee, Dong-Uk
AU  - Lee DU
AD  - Department of Immunopathology, College of Veterinary Medicine, Konkuk University, 
      120, Neungdong-ro, Gwangjin-gu, Seoul, South Korea.
FAU - Yoo, Sung J
AU  - Yoo SJ
AD  - Department of Immunopathology, College of Veterinary Medicine, Konkuk University, 
      120, Neungdong-ro, Gwangjin-gu, Seoul, South Korea.
FAU - Kwon, Taeyong
AU  - Kwon T
AD  - Department of Immunopathology, College of Veterinary Medicine, Konkuk University, 
      120, Neungdong-ro, Gwangjin-gu, Seoul, South Korea.
FAU - Je, Sang H
AU  - Je SH
AD  - Department of Immunopathology, College of Veterinary Medicine, Konkuk University, 
      120, Neungdong-ro, Gwangjin-gu, Seoul, South Korea.
FAU - Shin, Jeong Y
AU  - Shin JY
AD  - Department of Immunopathology, College of Veterinary Medicine, Konkuk University, 
      120, Neungdong-ro, Gwangjin-gu, Seoul, South Korea.
FAU - Byun, Jeong J
AU  - Byun JJ
AD  - Department of Immunopathology, College of Veterinary Medicine, Konkuk University, 
      120, Neungdong-ro, Gwangjin-gu, Seoul, South Korea.
FAU - Kim, Myung H
AU  - Kim MH
AD  - Hipra Korea Inc., 1601 ho, Intellige II B-Dong, Jeongiail-ro 177, Bundang-gu, 
      Seongnam-si, Gyeonggi-do, South Korea.
FAU - Lyoo, Young S
AU  - Lyoo YS
AD  - Department of Immunopathology, College of Veterinary Medicine, Konkuk University, 
      120, Neungdong-ro, Gwangjin-gu, Seoul, South Korea. Electronic address: 
      lyoo@konkuk.ac.kr.
LA  - eng
PT  - Journal Article
DEP - 20161221
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
SB  - IM
MH  - Animals
MH  - Austria
MH  - *Genetic Variation
MH  - Genotype
MH  - Germany
MH  - Open Reading Frames/*genetics
MH  - Phylogeny
MH  - Porcine Reproductive and Respiratory Syndrome/*virology
MH  - Porcine respiratory and reproductive syndrome virus/classification/*genetics
MH  - Republic of Korea
MH  - Swine
OTO - NOTNLM
OT  - Evolution
OT  - Genetic diversity
OT  - ORF4-6
OT  - PRRSV
OT  - Porcine reproductive and respiratory syndrome virus
EDAT- 2017/01/24 06:00
MHDA- 2017/02/28 06:00
CRDT- 2017/01/24 06:00
PHST- 2015/12/23 00:00 [received]
PHST- 2016/12/19 00:00 [revised]
PHST- 2016/12/20 00:00 [accepted]
PHST- 2017/01/24 06:00 [entrez]
PHST- 2017/01/24 06:00 [pubmed]
PHST- 2017/02/28 06:00 [medline]
AID - S0378-1135(16)30831-8 [pii]
AID - 10.1016/j.vetmic.2016.12.026 [doi]
PST - ppublish
SO  - Vet Microbiol. 2017 Feb;199:54-61. doi: 10.1016/j.vetmic.2016.12.026. Epub 2016 
      Dec 21.