PMID- 28114402
OWN - NLM
STAT- MEDLINE
DCOM- 20170814
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 1
DP  - 2017
TI  - The Anti-Oxidant Ergothioneine Augments the Immunomodulatory Function of TLR 
      Agonists by Direct Action on Macrophages.
PG  - e0169360
LID - 10.1371/journal.pone.0169360 [doi]
LID - e0169360
AB  - L-Ergothioneine (EGT) is a naturally-occurring amino acid which is characterized 
      by its antioxidant property; yet, the physiological role of EGT has yet to be 
      established. We investigated the immune-enhancing properties of EGT, and found 
      that it acts as a potentiator of toll-like receptor (TLR) signaling. When mouse 
      bone marrow-derived macrophages (BMDMs) were pretreated with EGT, TLR 
      signal-mediated cytokine production was augmented in BMDMs. The results were 
      reproducible with TLR2, 3, 4 and 7 agonists. In particular, IL-6 and IL-12p40 
      were elevated further by pretreatment with EGT in BMDMs, suggesting the induction 
      of M1 polarization. In co-culture assay with OT-II CD4+ T cells and splenic 
      F4/80+ macrophages, EGT significantly induced Th17 skewing in CD4+ T cells. Thus, 
      EGT is an immune modifier as well as a redox controller under TLR stimulation 
      that induces M1 macrophages and a Th17 shift in inflammation.
FAU - Yoshida, Sumito
AU  - Yoshida S
AD  - Department of Microbiology and Immunology, Graduate School of Medicine, Hokkaido 
      University, Kita-ku, Sapporo City, Japan.
AD  - Department of Pathology I, Graduate School of Medicine, Hokkaido University, 
      Kita-ku, Sapporo City, Japan.
FAU - Shime, Hiroaki
AU  - Shime H
AD  - Department of Microbiology and Immunology, Graduate School of Medicine, Hokkaido 
      University, Kita-ku, Sapporo City, Japan.
FAU - Funami, Kenji
AU  - Funami K
AD  - Department of Microbiology and Immunology, Graduate School of Medicine, Hokkaido 
      University, Kita-ku, Sapporo City, Japan.
FAU - Takaki, Hiromi
AU  - Takaki H
AD  - Department of Microbiology and Immunology, Graduate School of Medicine, Hokkaido 
      University, Kita-ku, Sapporo City, Japan.
FAU - Matsumoto, Misako
AU  - Matsumoto M
AD  - Department of Microbiology and Immunology, Graduate School of Medicine, Hokkaido 
      University, Kita-ku, Sapporo City, Japan.
AD  - Department of Pathology I, Graduate School of Medicine, Hokkaido University, 
      Kita-ku, Sapporo City, Japan.
FAU - Kasahara, Masanori
AU  - Kasahara M
AD  - Department of Pathology I, Graduate School of Medicine, Hokkaido University, 
      Kita-ku, Sapporo City, Japan.
FAU - Seya, Tsukasa
AU  - Seya T
AD  - Department of Microbiology and Immunology, Graduate School of Medicine, Hokkaido 
      University, Kita-ku, Sapporo City, Japan.
AD  - Department of Pathology I, Graduate School of Medicine, Hokkaido University, 
      Kita-ku, Sapporo City, Japan.
LA  - eng
PT  - Journal Article
DEP - 20170123
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Antioxidants)
RN  - 0 (Cytokines)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Toll-Like Receptors)
RN  - BDZ3DQM98W (Ergothioneine)
SB  - IM
MH  - Adjuvants, Immunologic/*pharmacology
MH  - Animals
MH  - Antioxidants/*pharmacology
MH  - Cells, Cultured
MH  - Cytokines/biosynthesis
MH  - Ergothioneine/*pharmacology
MH  - Female
MH  - Macrophages/*drug effects/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Reactive Oxygen Species/metabolism
MH  - Toll-Like Receptors/*agonists
PMC - PMC5256913
COIS- The authors received funding from Three B Co., Inc., a commercial company, in the 
      form of research materials for this study. There are no patents, products in 
      development or marketed products to declare. This does not alter the authors' 
      adherence to all the PLOS ONE policies on sharing data and materials.
EDAT- 2017/01/24 06:00
MHDA- 2017/08/15 06:00
PMCR- 2017/01/23
CRDT- 2017/01/24 06:00
PHST- 2016/07/07 00:00 [received]
PHST- 2016/12/15 00:00 [accepted]
PHST- 2017/01/24 06:00 [entrez]
PHST- 2017/01/24 06:00 [pubmed]
PHST- 2017/08/15 06:00 [medline]
PHST- 2017/01/23 00:00 [pmc-release]
AID - PONE-D-16-27125 [pii]
AID - 10.1371/journal.pone.0169360 [doi]
PST - epublish
SO  - PLoS One. 2017 Jan 23;12(1):e0169360. doi: 10.1371/journal.pone.0169360. 
      eCollection 2017.