PMID- 28115391
OWN - NLM
STAT- MEDLINE
DCOM- 20170905
LR  - 20170906
IS  - 1945-239X (Electronic)
IS  - 0021-9665 (Linking)
VI  - 55
IP  - 3
DP  - 2017 Mar 1
TI  - Determination of Miroestrol and Isomiroestrol From Pueraria mirifica (White Kwao 
      Krua) in Dietary Supplements by LC-MS-MS and LC-Q-Orbitrap/MS.
PG  - 214-221
LID - 10.1093/chromsci/bmw171 [doi]
AB  - The purpose of this study is to develop LC-MS-MS and LC-Q-Orbitrap/MS method for 
      the analysis of the components of Pueraria mirifica, which are illegal additives 
      in dietary supplements. Blank samples and samples spiked with miroestrol and 
      isomiroestrol were used for the initial development and validation studies. 
      Specificity, linearity, limit of quantification (LOQ), limit of detection (LOD), 
      accuracy, precision, recovery and stability were employed as the validation 
      parameters. The LODs of miroestrol and isomiroestrol were found to be 4.17 and 
      0.84 ng/mL, respectively, whereas their LOQs were 12.50 and 2.52 ng/mL, 
      respectively. The determination coefficient was over 0.999, intra- and inter-day 
      precisions were 0.8-6.9 and 1.9-9.8%, respectively, and intra and inter-day 
      accuracies were 82.1-103.7 and 85.0-109.7%, respectively. The mean recoveries of 
      the targeted compounds from the dietary supplements ranged from 86.9 to 108.9%. 
      The relative standard deviations (RSDs) for recovery were <5.8%. On the other 
      hand, the RSD of stability was <11.0%. Eight dietary supplements were tested 
      using the newly developed and validated method, out of which six were found to be 
      adulterated samples.
CI  - (c) The Author 2017. Published by Oxford University Press. All rights reserved. For 
      Permissions, please email: journals.permissions@oup.com.
FAU - Lee, Ji Hyun
AU  - Lee JH
AD  - Advanced Analysis Team, National Institute of Food and Drug Safety Evaluation, 
      Ministry of Food and Drug Safety, 187 Osongsaengmyeong2-ro, Osongeup, 
      Heungdeok-gu, Cheongju-si, Chungcheongbuk-do363-700, Republic of Korea.
FAU - Kim, Jung Yeon
AU  - Kim JY
AD  - Advanced Analysis Team, National Institute of Food and Drug Safety Evaluation, 
      Ministry of Food and Drug Safety, 187 Osongsaengmyeong2-ro, Osongeup, 
      Heungdeok-gu, Cheongju-si, Chungcheongbuk-do363-700, Republic of Korea.
FAU - Cho, So-Hyun
AU  - Cho SH
AD  - Advanced Analysis Team, National Institute of Food and Drug Safety Evaluation, 
      Ministry of Food and Drug Safety, 187 Osongsaengmyeong2-ro, Osongeup, 
      Heungdeok-gu, Cheongju-si, Chungcheongbuk-do363-700, Republic of Korea.
FAU - Jeong, Ji Hye
AU  - Jeong JH
AD  - Advanced Analysis Team, National Institute of Food and Drug Safety Evaluation, 
      Ministry of Food and Drug Safety, 187 Osongsaengmyeong2-ro, Osongeup, 
      Heungdeok-gu, Cheongju-si, Chungcheongbuk-do363-700, Republic of Korea.
FAU - Cho, Sooyeul
AU  - Cho S
AD  - Advanced Analysis Team, National Institute of Food and Drug Safety Evaluation, 
      Ministry of Food and Drug Safety, 187 Osongsaengmyeong2-ro, Osongeup, 
      Heungdeok-gu, Cheongju-si, Chungcheongbuk-do363-700, Republic of Korea.
FAU - Park, Hyoung Joon
AU  - Park HJ
AD  - Advanced Analysis Team, National Institute of Food and Drug Safety Evaluation, 
      Ministry of Food and Drug Safety, 187 Osongsaengmyeong2-ro, Osongeup, 
      Heungdeok-gu, Cheongju-si, Chungcheongbuk-do363-700, Republic of Korea.
FAU - Baek, Sun Young
AU  - Baek SY
AD  - Advanced Analysis Team, National Institute of Food and Drug Safety Evaluation, 
      Ministry of Food and Drug Safety, 187 Osongsaengmyeong2-ro, Osongeup, 
      Heungdeok-gu, Cheongju-si, Chungcheongbuk-do363-700, Republic of Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Chromatogr Sci
JT  - Journal of chromatographic science
JID - 0173225
RN  - 0 (Plant Extracts)
RN  - 0 (Steroids)
RN  - 0 (miroestrol)
SB  - IM
MH  - Chromatography, Liquid/*methods
MH  - Dietary Supplements/*analysis
MH  - Limit of Detection
MH  - Linear Models
MH  - Plant Extracts/*chemistry
MH  - Pueraria/*chemistry
MH  - Reproducibility of Results
MH  - Steroids/*analysis
MH  - Tandem Mass Spectrometry/*methods
EDAT- 2017/01/25 06:00
MHDA- 2017/09/07 06:00
CRDT- 2017/01/25 06:00
PHST- 2015/09/30 00:00 [received]
PHST- 2017/01/25 06:00 [pubmed]
PHST- 2017/09/07 06:00 [medline]
PHST- 2017/01/25 06:00 [entrez]
AID - bmw171 [pii]
AID - 10.1093/chromsci/bmw171 [doi]
PST - ppublish
SO  - J Chromatogr Sci. 2017 Mar 1;55(3):214-221. doi: 10.1093/chromsci/bmw171.