PMID- 28117274
OWN - NLM
STAT- MEDLINE
DCOM- 20180222
LR  - 20181024
IS  - 1878-7436 (Electronic)
IS  - 1878-7436 (Linking)
VI  - 11
IP  - 3
DP  - 2017 Mar
TI  - Schisandrin B displays a protective role against primary pulmonary hypertension 
      by targeting transforming growth factor beta1.
PG  - 148-157.e1
LID - S1933-1711(16)30606-4 [pii]
LID - 10.1016/j.jash.2016.12.007 [doi]
AB  - Pulmonary arterial smooth muscle cells (PASMCs) in the medial layer of the vessel 
      wall are involved in vessel homeostasis, but also for pathologic vascular 
      remodeling in diverse diseases, such as pulmonary arterial hypertension (PAH). 
      Pulmonary vascular remodeling in PAH results in vascular disorders, but its 
      underlying molecular mechanisms are still not to be fully disclosed. In this 
      study, we investigated the expression and function of the transforming growth 
      factor (TGF)-beta1 in human PASMC cultured under the condition of hypoxia and 
      elucidated the effect of schisandra chinensis and its active ingredients on 
      proliferation, migration, and apoptosis in human PASMCs. We demonstrated that 
      schisandrin B (Sch.B) alleviated the severity of PAH in PASMCs cultured under the 
      condition of hypoxia. Significant upregulation of TGF-beta1 was observed in 
      hypoxia-induced human PASMCs. Interestingly, administration of Sch.B 
      substantially attenuated TGF-beta1 level in these PASMCs. In order to elucidate 
      Sch.B function, the hypoxia-induced human PASMC was stimulated with Sch.B or 
      cotreatment with TGF-beta1 in vitro. In agreement with its TGF-beta1-reducing effect, 
      Sch B relieved human PASMCs migration and promoted the apoptosis of human PASMCs, 
      by activation of TGF-beta1 downstream signal pathways in PASMCs. In contrast, 
      co-treatment with TGF-beta1 promoted human PASMC proliferation and migration and 
      inhibited the apoptosis of human PASMC, which can attenuate the protective role 
      of Sch.B in human PASMC. Taken collectively, these findings suggest that the 
      vascular relaxation evoked by Sch.B was mediated by direct effect on vascular 
      smooth muscle cell via TGF-beta1 downstream signal pathways.
CI  - Copyright (c) 2016 American Society of Hypertension. Published by Elsevier Inc. All 
      rights reserved.
FAU - Wu, Jianjun
AU  - Wu J
AD  - Department of Cardiology, The Fourth Hospital of Harbin Medical University, 
      Harbin, Heilongjiang, China.
FAU - Jia, Jing
AU  - Jia J
AD  - Department of Cardiology, The Fourth Hospital of Harbin Medical University, 
      Harbin, Heilongjiang, China.
FAU - Liu, Li
AU  - Liu L
AD  - Department of Anesthesiology, The Third Hospital of Harbin Medical University, 
      Harbin, Heilongjiang, China; Key Laboratory of Cardiovascular Medicine Research, 
      Harbin, Heilongjiang, China.
FAU - Yang, Fan
AU  - Yang F
AD  - Department of Pathophysiology, Harbin Medical University, Harbin, China.
FAU - Fan, Yuhua
AU  - Fan Y
AD  - Department of Pharmacy, Harbin Medical University, Harbin, China.
FAU - Zhang, Sen
AU  - Zhang S
AD  - Department of Cardiology, The Fourth Hospital of Harbin Medical University, 
      Harbin, Heilongjiang, China.
FAU - Yan, Dongxia
AU  - Yan D
AD  - Department of Cardiology, The Fourth Hospital of Harbin Medical University, 
      Harbin, Heilongjiang, China.
FAU - Bu, Rui
AU  - Bu R
AD  - Department of Cardiology, The Fourth Hospital of Harbin Medical University, 
      Harbin, Heilongjiang, China.
FAU - Li, Guangnan
AU  - Li G
AD  - Department of Cardiology, The Fourth Hospital of Harbin Medical University, 
      Harbin, Heilongjiang, China.
FAU - Gao, Yanhui
AU  - Gao Y
AD  - Department of Cardiology, The Fourth Hospital of Harbin Medical University, 
      Harbin, Heilongjiang, China.
FAU - Chen, Yanjun
AU  - Chen Y
AD  - Department of Cardiology, The Fourth Hospital of Harbin Medical University, 
      Harbin, Heilongjiang, China. Electronic address: chenyanjunhyd@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20161228
PL  - United States
TA  - J Am Soc Hypertens
JT  - Journal of the American Society of Hypertension : JASH
JID - 101312518
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Cyclooctanes)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Lignans)
RN  - 0 (Polycyclic Compounds)
RN  - 0 (TGFB1 protein, human)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 02XA4X3KZW (schizandrin B)
SB  - IM
MH  - Antineoplastic Agents/*pharmacology/therapeutic use
MH  - Apoptosis/drug effects
MH  - Cell Movement/drug effects
MH  - Cells, Cultured
MH  - Cyclooctanes/pharmacology/therapeutic use
MH  - Dose-Response Relationship, Drug
MH  - Drugs, Chinese Herbal
MH  - Humans
MH  - Hypertension, Pulmonary/*drug therapy/pathology
MH  - Hypoxia/complications/pathology
MH  - Immunochemistry
MH  - Lignans/*pharmacology/therapeutic use
MH  - Medicine, Chinese Traditional/methods
MH  - Muscle, Smooth, Vascular
MH  - Myocytes, Smooth Muscle
MH  - Polycyclic Compounds/*pharmacology/therapeutic use
MH  - Pulmonary Artery/cytology
MH  - Schisandra/chemistry
MH  - Signal Transduction/drug effects
MH  - Transforming Growth Factor beta1/*metabolism
MH  - Up-Regulation
MH  - Vascular Remodeling/*drug effects
OTO - NOTNLM
OT  - Apoptosis
OT  - proliferation
OT  - pulmonary arterial hypertension
OT  - schisandra chinensis
EDAT- 2017/01/25 06:00
MHDA- 2018/02/23 06:00
CRDT- 2017/01/25 06:00
PHST- 2016/06/20 00:00 [received]
PHST- 2016/12/09 00:00 [revised]
PHST- 2016/12/18 00:00 [accepted]
PHST- 2017/01/25 06:00 [pubmed]
PHST- 2018/02/23 06:00 [medline]
PHST- 2017/01/25 06:00 [entrez]
AID - S1933-1711(16)30606-4 [pii]
AID - 10.1016/j.jash.2016.12.007 [doi]
PST - ppublish
SO  - J Am Soc Hypertens. 2017 Mar;11(3):148-157.e1. doi: 10.1016/j.jash.2016.12.007. 
      Epub 2016 Dec 28.