PMID- 28117817
OWN - NLM
STAT- MEDLINE
DCOM- 20170927
LR  - 20181202
IS  - 1940-087X (Electronic)
IS  - 1940-087X (Linking)
IP  - 119
DP  - 2017 Jan 11
TI  - Detection of Inter-chromosomal Stable Aberrations by Multiple Fluorescence In 
      Situ Hybridization (mFISH) and Spectral Karyotyping (SKY) in Irradiated Mice.
LID - 10.3791/55162 [doi]
LID - 55162
AB  - Ionizing radiation (IR) induces numerous stable and unstable chromosomal 
      aberrations. Unstable aberrations, where chromosome morphology is substantially 
      compromised, can easily be identified by conventional chromosome staining 
      techniques. However, detection of stable aberrations, which involve exchange or 
      translocation of genetic materials without considerable modification in the 
      chromosome morphology, requires sophisticated chromosome painting techniques that 
      rely on in situ hybridization of fluorescently labeled DNA probes, a chromosome 
      painting technique popularly known as fluorescence in situ hybridization (FISH). 
      FISH probes can be specific for whole chromosome/s or precise sub-region on 
      chromosome/s. The method not only allows visualization of stable aberrations, but 
      it can also allow detection of the chromosome/s or specific DNA sequence/s 
      involved in a particular aberration formation. A variety of chromosome painting 
      techniques are available in cytogenetics; here two highly sensitive methods, 
      multiple fluorescence in situ hybridization (mFISH) and spectral karyotyping 
      (SKY), are discussed to identify inter-chromosomal stable aberrations that form 
      in the bone marrow cells of mice after exposure to total body irradiation. 
      Although both techniques rely on fluorescent labeled DNA probes, the method of 
      detection and the process of image acquisition of the fluorescent signals are 
      different. These two techniques have been used in various research areas, such as 
      radiation biology, cancer cytogenetics, retrospective radiation biodosimetry, 
      clinical cytogenetics, evolutionary cytogenetics, and comparative cytogenetics.
FAU - Pathak, Rupak
AU  - Pathak R
AD  - Division of Radiation Health, Department of Pharmaceutical Sciences, College of 
      Pharmacy, University of Arkansas for Medical Sciences; RPathak@uams.edu.
FAU - Koturbash, Igor
AU  - Koturbash I
AD  - Department of Environmental Health, Fay W. Boozman School of Public Health, 
      University of Arkansas for Medical Sciences.
FAU - Hauer-Jensen, Martin
AU  - Hauer-Jensen M
AD  - Division of Radiation Health, Department of Pharmaceutical Sciences, College of 
      Pharmacy, University of Arkansas for Medical Sciences; Surgical Service, Central 
      Arkansas Veterans Healthcare System.
LA  - eng
GR  - P20 GM109005/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Video-Audio Media
DEP - 20170111
PL  - United States
TA  - J Vis Exp
JT  - Journal of visualized experiments : JoVE
JID - 101313252
RN  - 0 (DNA Probes)
RN  - 0 (Fluorescent Dyes)
SB  - IM
MH  - Animals
MH  - *Chromosome Aberrations
MH  - Chromosome Painting
MH  - DNA Probes
MH  - Fluorescent Dyes
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Mice
MH  - Spectral Karyotyping/*methods
MH  - Translocation, Genetic
PMC - PMC5352253
EDAT- 2017/01/25 06:00
MHDA- 2017/09/28 06:00
PMCR- 2018/01/11
CRDT- 2017/01/25 06:00
PHST- 2017/01/25 06:00 [entrez]
PHST- 2017/01/25 06:00 [pubmed]
PHST- 2017/09/28 06:00 [medline]
PHST- 2018/01/11 00:00 [pmc-release]
AID - 55162 [pii]
AID - 10.3791/55162 [doi]
PST - epublish
SO  - J Vis Exp. 2017 Jan 11;(119):55162. doi: 10.3791/55162.