PMID- 28118564
OWN - NLM
STAT- MEDLINE
DCOM- 20170606
LR  - 20190729
IS  - 1944-7922 (Electronic)
IS  - 1060-3271 (Linking)
VI  - 100
IP  - 2
DP  - 2017 Mar 1
TI  - A Label-Free Electrochemical Biosensor Based on a Reduced Graphene Oxide and 
      Indole-5-Carboxylic Acid Nanocomposite for the Detection of Klebsiella 
      pneumoniae.
PG  - 548-552
LID - 10.5740/jaoacint.16-0251 [doi]
AB  - A label-free DNA hybridization electrochemical sensor for the detection of 
      Klebsiella pneumoniae was developed, which could be helpful in the diagnosis of 
      bacterial infections. Indole-5-carboxylic acid (ICA) and graphene oxide (GO) were 
      electrodeposited on a glassy carbon electrode, and the resulting reduced GO 
      (rGO)-ICA hybrid film served as a platform for immobilizing oligonucleotides on a 
      single-stranded DNA (ssDNA) sequence. The conditions were optimized, with 
      excellent electrochemical performance. A significant change was observed after 
      hybridization of ssDNA with the target probe under optimum conditions. 
      Hybridization with complementary, noncomplementary, one-base mismatched, and 
      three-base mismatched DNA targets was studied effectively by differential pulse 
      voltammetry. The proposed strategy could detect target DNA down to 3 x 10-11 M, 
      with a linear range from 1 x 10-6 M to 1 x 10-10 M, showing high sensitivity. 
      This electrochemical method is simple, free from indicator, and shows good 
      selectivity. Hence, electrochemical biosensors are successfully demonstrated for 
      the detection of K. pneumoniae.
FAU - Zhang, Ze
AU  - Zhang Z
AD  - Fudan University Pudong Medical Center, Shanghai Pudong Hospital, Department of 
      Clinical Laboratory, 2800 Gongwei Rd, Huinan Town, Pudong, Shanghai 201399, 
      China.
FAU - Yu, Hong-Wei
AU  - Yu HW
AD  - The First Affiliated Hospital of the Harbin Medical University, No. 23 Youzheng 
      St, Harbin, Heilongjiang Province 150001, China.
FAU - Wan, Guang-Cai
AU  - Wan GC
AD  - The First Affiliated Hospital of the Harbin Medical University, No. 23 Youzheng 
      St, Harbin, Heilongjiang Province 150001, China.
FAU - Jiang, Jing-Hui
AU  - Jiang JH
AD  - The First Affiliated Hospital of the Harbin Medical University, No. 23 Youzheng 
      St, Harbin, Heilongjiang Province 150001, China.
FAU - Wang, Na
AU  - Wang N
AD  - The First Affiliated Hospital of the Harbin Medical University, No. 23 Youzheng 
      St, Harbin, Heilongjiang Province 150001, China.
FAU - Liu, Zhi-Yong
AU  - Liu ZY
AD  - The First Hospital of Suihua City, Intersection of Renhe St and Changjiang North 
      Rd, Suihua, Heilongjiang Province 152000, China.
FAU - Chang, Dong
AU  - Chang D
AD  - Fudan University Pudong Medical Center, Shanghai Pudong Hospital, Department of 
      Clinical Laboratory, 2800 Gongwei Rd, Huinan Town, Pudong, Shanghai 201399, 
      China.
FAU - Pan, Hong-Zhi
AU  - Pan HZ
AD  - Harbin Medical University, Public Health School, No. 157 Baojian Rd, Nangang 
      District, Harbin, Heilongjiang Province 150081, China.
LA  - eng
PT  - Journal Article
DEP - 20161130
PL  - England
TA  - J AOAC Int
JT  - Journal of AOAC International
JID - 9215446
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Single-Stranded)
RN  - 0 (Indoles)
RN  - 0 (indole-5-carboxylic acid)
RN  - 7782-42-5 (Graphite)
SB  - IM
MH  - Biosensing Techniques/*methods
MH  - DNA Probes/chemistry
MH  - DNA, Single-Stranded/chemistry
MH  - Electrochemical Techniques/*methods
MH  - Graphite/*chemistry
MH  - Indoles/*chemistry
MH  - Klebsiella pneumoniae/genetics/*isolation & purification
MH  - Nanocomposites/*chemistry
MH  - Nucleic Acid Hybridization
EDAT- 2017/01/25 06:00
MHDA- 2017/06/07 06:00
CRDT- 2017/01/25 06:00
PHST- 2017/01/25 06:00 [pubmed]
PHST- 2017/06/07 06:00 [medline]
PHST- 2017/01/25 06:00 [entrez]
AID - 10.5740/jaoacint.16-0251 [doi]
PST - ppublish
SO  - J AOAC Int. 2017 Mar 1;100(2):548-552. doi: 10.5740/jaoacint.16-0251. Epub 2016 
      Nov 30.