PMID- 28118755
OWN - NLM
STAT- MEDLINE
DCOM- 20170217
LR  - 20181113
IS  - 1475-6374 (Electronic)
IS  - 1475-6366 (Print)
IS  - 1475-6366 (Linking)
VI  - 32
IP  - 1
DP  - 2017 Dec
TI  - Expression of heterologous oxalate decarboxylase in HEK293 cells confers 
      protection against oxalate induced oxidative stress as a therapeutic approach for 
      calcium oxalate stone disease.
PG  - 426-433
LID - 10.1080/14756366.2016.1256884 [doi]
AB  - Oxalates stimulate alterations in renal epithelial cells and thereby induce 
      calcium oxalate (CaOx) stone formation. Bacillus subtilis YvrK gene encodes for 
      oxalate decarboxylase (OxdC) which degrades oxalate to formate and CO(2). The 
      present work is aimed to clone the oxdC gene in a mammalian expression vector 
      pcDNA and transfect into Human Embryonic Kidney 293 (HEK293) cells and evaluate 
      the oxdC expression, cell survival rate and oxalate degrading efficiency. The 
      results indicate cell survival rate of HEK293/pcDNAOXDC cells pre-incubated with 
      oxalate was enhanced by 28%. HEK293/pcDNAOXDC cells expressing OxdC treated with 
      oxalate, significantly restored antioxidant activity, mitochondrial membrane 
      potential and intracellular reactive oxygen species (ROS) generation compared 
      with HEK293/pcDNA. Apoptotic marker caspase 3 downregulation illustrates 
      HEK293/pcDNAOXDC cells were able to survive under oxalate-mediated oxidative 
      stress. The findings suggest HEK293 cells expressing oxdC capable of degrading 
      oxalate protect cells from oxidative damage and thus serve as a therapeutic 
      option for prevention of CaOx stone disease. [Formula: see text].
FAU - Albert, Abhishek
AU  - Albert A
AD  - a Department of Biochemistry, Centre for Excellence in Genomics Science , School 
      of Biological Sciences, Madurai Kamaraj University , Madurai , India.
FAU - Tiwari, Vidhi
AU  - Tiwari V
AD  - a Department of Biochemistry, Centre for Excellence in Genomics Science , School 
      of Biological Sciences, Madurai Kamaraj University , Madurai , India.
FAU - Paul, Eldho
AU  - Paul E
AD  - a Department of Biochemistry, Centre for Excellence in Genomics Science , School 
      of Biological Sciences, Madurai Kamaraj University , Madurai , India.
FAU - Ganesan, Divya
AU  - Ganesan D
AD  - a Department of Biochemistry, Centre for Excellence in Genomics Science , School 
      of Biological Sciences, Madurai Kamaraj University , Madurai , India.
FAU - Ayyavu, Mahesh
AU  - Ayyavu M
AD  - b DBT-IPLS Programme, School of Biological Science , Madurai Kamaraj University , 
      Madurai , India.
FAU - Kujur, Ritu
AU  - Kujur R
AD  - a Department of Biochemistry, Centre for Excellence in Genomics Science , School 
      of Biological Sciences, Madurai Kamaraj University , Madurai , India.
FAU - Ponnusamy, Sasikumar
AU  - Ponnusamy S
AD  - c Department of Oral Biology, School of Dental Medicine , University at Buffalo , 
      Buffalo , NY , USA.
FAU - Shanmugam, Kathiresan
AU  - Shanmugam K
AD  - d Department of Molecular Biology , Centre for Excellence in Genomics Science, 
      School of Biological Sciences, Madurai Kamaraj University , Madurai , India.
FAU - Saso, Luciano
AU  - Saso L
AD  - e Department of Physiology and Pharmacology "Vittorio Erspamer" , Sapienza 
      University of Rome , Rome , Italy.
FAU - Govindan Sadasivam, Selvam
AU  - Govindan Sadasivam S
AD  - a Department of Biochemistry, Centre for Excellence in Genomics Science , School 
      of Biological Sciences, Madurai Kamaraj University , Madurai , India.
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Enzyme Inhib Med Chem
JT  - Journal of enzyme inhibition and medicinal chemistry
JID - 101150203
RN  - 0 (Oxalates)
RN  - 0 (Reactive Oxygen Species)
RN  - 2612HC57YE (Calcium Oxalate)
SB  - IM
MH  - Calcium Oxalate/*chemistry
MH  - HEK293 Cells
MH  - Humans
MH  - Kidney Calculi/metabolism/*prevention & control
MH  - Membrane Potential, Mitochondrial
MH  - Oxalates/*pharmacology
MH  - Oxidative Stress/*drug effects
MH  - Reactive Oxygen Species/metabolism
PMC - PMC6010120
OTO - NOTNLM
OT  - Calcium oxalate
OT  - gene therapy
OT  - hyperoxaluria
OT  - oxalate decarboxylase
OT  - urolithiasis
EDAT- 2017/01/26 06:00
MHDA- 2017/02/18 06:00
PMCR- 2017/01/24
CRDT- 2017/01/26 06:00
PHST- 2017/01/26 06:00 [entrez]
PHST- 2017/01/26 06:00 [pubmed]
PHST- 2017/02/18 06:00 [medline]
PHST- 2017/01/24 00:00 [pmc-release]
AID - 1256884 [pii]
AID - 10.1080/14756366.2016.1256884 [doi]
PST - ppublish
SO  - J Enzyme Inhib Med Chem. 2017 Dec;32(1):426-433. doi: 
      10.1080/14756366.2016.1256884.