PMID- 28119397
OWN - NLM
STAT- MEDLINE
DCOM- 20170906
LR  - 20191210
IS  - 1530-6860 (Electronic)
IS  - 0892-6638 (Linking)
VI  - 31
IP  - 4
DP  - 2017 Apr
TI  - Misdirection of endosomal trafficking mediated by herpes simplex virus-encoded 
      glycoprotein B.
PG  - 1650-1667
LID - 10.1096/fj.201600521R [doi]
AB  - Herpes simplex virus (HSV)-encoded glycoprotein B (gB) is the most abundant 
      protein in the viral envelope and promotes fusion of the virus with the cellular 
      membrane. In the present study, we found that gB impacts on the major 
      histocompatibility complex (MHC)-II pathway of antigen presentation by fostering 
      homotypic fusion of early endosomes and trapping MHC-II molecules in these 
      altered endosomes. By using an overexpression approach, we demonstrated that 
      transient expression of gB induces giant vesicles of early endosomal origin, 
      which contained Rab5, early endosomal antigen 1 (EEA1), and large amounts of 
      MHC-II molecules [human leukocyte antigen (HLA)-DR, and HLA-DM], but no CD63. In 
      HSV-1-infected and stably transfected cell lines that expressed lower amounts of 
      gB, giant endosomes were not observed, but strongly increased amounts of HLA-DR 
      and HLA-DM were found in EEA1(+) early endosomes. We used these giant vesicles as 
      a model system and revealed that gB interacts with Rab5 and EEA1, and that 
      gB-induced homotypic fusion of early endosomes to giant endosomes requires 
      phosphatidylinositol 3-phosphate, the activity of soluble 
      N-ethylmaleimide-sensitive factor attachment protein receptors, and the cytosolic 
      gB sequence (889)YTQVPN(894) We conclude that gB expression alters trafficking of 
      molecules of the HLA-II processing pathway, which leads to increased retention of 
      MHC-II molecules in early endosomal compartments, thereby intercepting antigen 
      presentation.-Niazy, N., Temme, S., Bocuk, D., Giesen, C., Konig, A., Temme, N., 
      Ziegfeld, A., Gregers, T. F., Bakke, O., Lang, T., Eis-Hubinger, A. M., Koch, N. 
      Misdirection of endosomal trafficking mediated by herpes simplex virus-encoded 
      glycoprotein B.
CI  - (c) FASEB.
FAU - Niazy, Naima
AU  - Niazy N
AD  - Section of Immunobiology, Institute of Genetics, University of Bonn, Bonn, 
      Germany.
FAU - Temme, Sebastian
AU  - Temme S
AD  - Section of Immunobiology, Institute of Genetics, University of Bonn, Bonn, 
      Germany; sebastian.Temme@uni-duesseldorf.de.
FAU - Bocuk, Derya
AU  - Bocuk D
AD  - Section of Immunobiology, Institute of Genetics, University of Bonn, Bonn, 
      Germany.
FAU - Giesen, Carmen
AU  - Giesen C
AD  - Section of Immunobiology, Institute of Genetics, University of Bonn, Bonn, 
      Germany.
FAU - Konig, Angelika
AU  - Konig A
AD  - Section of Immunobiology, Institute of Genetics, University of Bonn, Bonn, 
      Germany.
FAU - Temme, Nadine
AU  - Temme N
AD  - Section of Immunobiology, Institute of Genetics, University of Bonn, Bonn, 
      Germany.
FAU - Ziegfeld, Angelique
AU  - Ziegfeld A
AD  - Section of Immunobiology, Institute of Genetics, University of Bonn, Bonn, 
      Germany.
FAU - Gregers, Tone F
AU  - Gregers TF
AD  - Department of Biosciences, University of Oslo, Oslo, Norway.
FAU - Bakke, Oddmund
AU  - Bakke O
AD  - Department of Biosciences, University of Oslo, Oslo, Norway.
FAU - Lang, Thorsten
AU  - Lang T
AD  - Membrane Biochemistry, Life and Medical Sciences Institute, University of Bonn, 
      Bonn, Germany.
FAU - Eis-Hubinger, Anna M
AU  - Eis-Hubinger AM
AD  - Institute of Virology, University of Bonn Medical Centre, Bonn, Germany.
FAU - Koch, Norbert
AU  - Koch N
AD  - Section of Immunobiology, Institute of Genetics, University of Bonn, Bonn, 
      Germany.
LA  - eng
PT  - Journal Article
DEP - 20170124
PL  - United States
TA  - FASEB J
JT  - FASEB journal : official publication of the Federation of American Societies for 
      Experimental Biology
JID - 8804484
RN  - 0 (HLA-D Antigens)
RN  - 0 (HLA-DM antigens)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Phosphatidylinositol Phosphates)
RN  - 0 (Tetraspanin 30)
RN  - 0 (Vesicular Transport Proteins)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (early endosome antigen 1)
RN  - 0 (glycoprotein B, Simplexvirus)
RN  - 0 (phosphatidylinositol 3-phosphate)
RN  - EC 3.6.1.- (RAB5C protein, human)
RN  - EC 3.6.5.2 (rab5 GTP-Binding Proteins)
SB  - IM
MH  - Amino Acid Motifs
MH  - Animals
MH  - COS Cells
MH  - Chlorocebus aethiops
MH  - Endosomes/*metabolism/virology
MH  - HLA-D Antigens/metabolism
MH  - HLA-DR Antigens/metabolism
MH  - HeLa Cells
MH  - Humans
MH  - Phosphatidylinositol Phosphates/metabolism
MH  - Protein Transport
MH  - Tetraspanin 30/metabolism
MH  - Vesicular Transport Proteins/metabolism
MH  - Viral Envelope Proteins/chemistry/genetics/*metabolism
MH  - rab5 GTP-Binding Proteins/metabolism
OTO - NOTNLM
OT  - HLA class II
OT  - HSV-1
OT  - antigen processing compartments
OT  - giant endosomal vesicles
OT  - signal sequences
EDAT- 2017/01/26 06:00
MHDA- 2017/09/07 06:00
CRDT- 2017/01/26 06:00
PHST- 2016/08/13 00:00 [received]
PHST- 2017/01/01 00:00 [accepted]
PHST- 2017/01/26 06:00 [pubmed]
PHST- 2017/09/07 06:00 [medline]
PHST- 2017/01/26 06:00 [entrez]
AID - fj.201600521R [pii]
AID - 10.1096/fj.201600521R [doi]
PST - ppublish
SO  - FASEB J. 2017 Apr;31(4):1650-1667. doi: 10.1096/fj.201600521R. Epub 2017 Jan 24.