PMID- 28119977
OWN - NLM
STAT- MEDLINE
DCOM- 20170327
LR  - 20170327
IS  - 2042-650X (Electronic)
IS  - 2042-6496 (Linking)
VI  - 8
IP  - 2
DP  - 2017 Feb 22
TI  - Anti-inflammatory activity of 3beta-hydroxycholest-5-en-7-one isolated from 
      Hippocampus trimaculatus leach via inhibiting iNOS, TNF-alpha, and 1L-1beta of LPS 
      induced RAW 264.7 macrophage cells.
PG  - 788-795
LID - 10.1039/c6fo01154c [doi]
AB  - Hippocampus trimaculatus leach has been widely used in beverage and herbal 
      medicine fabrication. However, the study of the molecular mechanism of its 
      bioactivity especially the anti-inflammatory activity is still scanty. In the 
      present study, the pure HEO isolated from the dried Hippocampus trimaculatus 
      leach was firstly found to have anti-inflammatory activity in vitro. The 
      molecular mechanism of the anti-inflammatory activity was detailed using the 
      lipopolysaccharide (LPS) stimulated RAW 264.7 macrophage cells, suggesting that 
      the HEO can significantly suppress the inflammatory factors of nitric oxide 
      synthase (iNOS), tumor necrosis factor-alpha (TNF-alpha) and interleukin (1L)-1beta (1L-1beta). 
      Cellular signaling analyses demonstrated that the HEO downregulated the NF-kappaB and 
      extracellular signal-regulated kinase (ERK) of mitogen-activated protein kinase 
      (MAPK) signaling pathways. All the results suggested that the HEO is a potential 
      natural anti-inflammatory agent.
FAU - Wu, Jingna
AU  - Wu J
AUID- ORCID: 0000-0002-2397-8904
AD  - Institute of Next Generation Matter Transformation, College of Chemical 
      Engineering at Huaqiao University, 361021, Xiamen, P. R. China. qsong@hqu.edu.cn 
      and Fisheries Research Institute of Fujian, Fujian Collaborative Innovation 
      Center for Exploitation and Utilization of Marine Biological Resources/Key 
      Laboratory of Cultivation and High Value Utilization of Marine Organisms in 
      Fujian Province, 361013, Xiamen, P. R. China. 13906008638@163.com.
FAU - Liu, Zhiyu
AU  - Liu Z
AD  - Fisheries Research Institute of Fujian, Fujian Collaborative Innovation Center 
      for Exploitation and Utilization of Marine Biological Resources/Key Laboratory of 
      Cultivation and High Value Utilization of Marine Organisms in Fujian Province, 
      361013, Xiamen, P. R. China. 13906008638@163.com.
FAU - Su, Jie
AU  - Su J
AD  - Fisheries Research Institute of Fujian, Fujian Collaborative Innovation Center 
      for Exploitation and Utilization of Marine Biological Resources/Key Laboratory of 
      Cultivation and High Value Utilization of Marine Organisms in Fujian Province, 
      361013, Xiamen, P. R. China. 13906008638@163.com.
FAU - Pan, Nan
AU  - Pan N
AD  - Fisheries Research Institute of Fujian, Fujian Collaborative Innovation Center 
      for Exploitation and Utilization of Marine Biological Resources/Key Laboratory of 
      Cultivation and High Value Utilization of Marine Organisms in Fujian Province, 
      361013, Xiamen, P. R. China. 13906008638@163.com.
FAU - Song, Qiuling
AU  - Song Q
AUID- ORCID: 0000-0002-9836-8860
AD  - Institute of Next Generation Matter Transformation, College of Chemical 
      Engineering at Huaqiao University, 361021, Xiamen, P. R. China. qsong@hqu.edu.cn.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Food Funct
JT  - Food & function
JID - 101549033
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Cholestenones)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-kappa B)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/chemistry/isolation & purification/*pharmacology
MH  - Cholestenones/chemistry/isolation & purification/*pharmacology
MH  - Interleukin-1beta/genetics/*immunology
MH  - Lipopolysaccharides/immunology
MH  - Macrophages/*drug effects/immunology
MH  - Mice
MH  - Molecular Structure
MH  - NF-kappa B/genetics/*immunology
MH  - Nitric Oxide Synthase Type II/genetics/*immunology
MH  - RAW 264.7 Cells
MH  - *Smegmamorpha
MH  - Tumor Necrosis Factor-alpha/genetics/*immunology
EDAT- 2017/01/26 06:00
MHDA- 2017/03/28 06:00
CRDT- 2017/01/26 06:00
PHST- 2017/01/26 06:00 [pubmed]
PHST- 2017/03/28 06:00 [medline]
PHST- 2017/01/26 06:00 [entrez]
AID - 10.1039/c6fo01154c [doi]
PST - ppublish
SO  - Food Funct. 2017 Feb 22;8(2):788-795. doi: 10.1039/c6fo01154c.