PMID- 2812273
OWN - NLM
STAT- MEDLINE
DCOM- 19891201
LR  - 20180214
IS  - 0028-3835 (Print)
IS  - 0028-3835 (Linking)
VI  - 50
IP  - 4
DP  - 1989 Oct
TI  - Quantification of proteins in discrete brain regions of androgen-insensitive 
      testicular feminized Tfm mice.
PG  - 392-9
AB  - The effect of the testicular feminization mutation (Tfm) on the concentration of 
      specific proteins in the medial preoptic area (MPO), ventromedial hypothalamus 
      (VMH) and parietal cortex (CX) was examined. Adult Tfm and Swiss-Webster male 
      mice were decapitated, the brains were removed and sectioned. Proteins from the 
      three microdissected areas were separated by two-dimensional gel electrophoresis. 
      Gels were stained with silver and then analyzed by quantitative computerized 
      scanning densitometry. Of the 195 proteins quantified, the Tfm mutation 
      significantly influenced the concentration of 16 proteins measured from gels of 
      MPO tissue, 21 from VMH gels and 11 from CX. Of these, three proteins were 
      affected in all brain regions; and three additional proteins were shown to vary 
      in both MPO and VMH. One protein higher in the MPO and VMH of Tfm mice was 
      identified as the glial fibrillary acidic protein. It is suggested that the 
      proteins influenced by the Tfm mutation are regulated by steroids, most likely 
      androgens. Thus, these proteins may prove to be important in hormone-regulated 
      physiological functions.
FAU - Olsen, K L
AU  - Olsen KL
AD  - Department of Psychiatry and Behavioral Science, SUNY.
FAU - Heydorn, W E
AU  - Heydorn WE
FAU - Rodriguez-Sierra, J F
AU  - Rodriguez-Sierra JF
FAU - Jacobowitz, D M
AU  - Jacobowitz DM
LA  - eng
GR  - HD 13219/HD/NICHD NIH HHS/United States
GR  - HD 18893/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Switzerland
TA  - Neuroendocrinology
JT  - Neuroendocrinology
JID - 0035665
RN  - 0 (Androgens)
RN  - 0 (Nerve Tissue Proteins)
SB  - IM
MH  - Androgen-Insensitivity Syndrome/*metabolism
MH  - Androgens/*physiology
MH  - Animals
MH  - Cerebral Cortex/*metabolism/physiology
MH  - Hypothalamus/*metabolism/physiology
MH  - Male
MH  - Mice
MH  - Mice, Mutant Strains
MH  - Nerve Tissue Proteins/*metabolism/physiology
EDAT- 1989/10/01 00:00
MHDA- 1989/10/01 00:01
CRDT- 1989/10/01 00:00
PHST- 1989/10/01 00:00 [pubmed]
PHST- 1989/10/01 00:01 [medline]
PHST- 1989/10/01 00:00 [entrez]
AID - 10.1159/000125254 [doi]
PST - ppublish
SO  - Neuroendocrinology. 1989 Oct;50(4):392-9. doi: 10.1159/000125254.