PMID- 28122731
OWN - NLM
STAT- MEDLINE
DCOM- 20170605
LR  - 20231213
IS  - 1522-1563 (Electronic)
IS  - 0363-6143 (Print)
IS  - 0363-6143 (Linking)
VI  - 312
IP  - 4
DP  - 2017 Apr 1
TI  - Mitochondrial connexin40 regulates mitochondrial calcium uptake in coronary 
      endothelial cells.
PG  - C398-C406
LID - 10.1152/ajpcell.00283.2016 [doi]
AB  - Connexins (Cxs) are a group of integral membrane proteins that can form gap 
      junctions between adjacent cells. Recently, it was reported that Cx43 is 
      expressed not only in the plasma membrane but also in the inner mitochondrial 
      membrane and that it regulates mitochondrial functions. Cx40 is predominantly 
      expressed in vascular endothelial cells (ECs) and plays an important role in the 
      electrical propagation between ECs and endothelial/smooth muscle cells. However, 
      it is unknown whether Cx40 is expressed in the mitochondria and what the role of 
      mitochondrial Cx40 is in endothelial functions. We observed in coronary ECs that 
      Cx40 protein was expressed in the mitochondria, as determined by Western blot and 
      immunofluorescence studies. We found that mouse coronary ECs (MCECs) isolated 
      from Cx40 knockout (Cx40 KO) mice exhibited significantly lower resting 
      mitochondrial calcium concentration ([Ca(2+)](mito)) than MCECs from wild-type 
      (WT) mice. After increase in cytosolic Ca(2+) concentration ([Ca(2+)](cyto)) with 
      cyclopiazonic acid, calcium uptake into the mitochondria was significantly 
      attenuated in MCECs from Cx40 KO mice compared with WT MCECs. There was no 
      difference in resting [Ca(2+)](cyto) and store-operated calcium entry in MCECs 
      from WT and Cx40 KO mice. We also detected a significant decrease in the 
      concentration of mitochondrial reactive oxygen species (ROS) in Cx40 KO MCECs. 
      Cx40 overexpression in ECs significantly increased resting [Ca(2+)](mito) level 
      and calcium uptake by mitochondria in response to increased [Ca(2+)](cyto) and 
      augmented mitochondrial ROS production. These data suggest that mitochondrial 
      Cx40 contributes to the regulation of mitochondrial calcium homeostasis.
CI  - Copyright (c) 2017 the American Physiological Society.
FAU - Guo, Rui
AU  - Guo R
AD  - Department of Physiology, The University of Arizona, Tucson, Arizona; and.
FAU - Si, Rui
AU  - Si R
AD  - Department of Physiology, The University of Arizona, Tucson, Arizona; and.
FAU - Scott, Brian T
AU  - Scott BT
AD  - Department of Medicine, University of California, San Diego, La Jolla, 
      California.
FAU - Makino, Ayako
AU  - Makino A
AD  - Department of Physiology, The University of Arizona, Tucson, Arizona; and 
      aymakino@email.arizona.edu.
LA  - eng
GR  - R01 HL115578/HL/NHLBI NIH HHS/United States
GR  - S10 OD019948/OD/NIH HHS/United States
PT  - Journal Article
DEP - 20170125
PL  - United States
TA  - Am J Physiol Cell Physiol
JT  - American journal of physiology. Cell physiology
JID - 100901225
RN  - 0 (Connexins)
RN  - 0 (Mitochondrial Proteins)
RN  - 0 (Reactive Oxygen Species)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Calcium/*metabolism
MH  - Calcium Signaling/*physiology
MH  - Cells, Cultured
MH  - Connexins/genetics/*metabolism
MH  - Coronary Vessels/cytology/*metabolism
MH  - Endothelial Cells/cytology/*metabolism
MH  - Gene Expression Regulation/physiology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Mitochondria, Heart/*metabolism
MH  - Mitochondrial Proteins/metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - Gap Junction alpha-5 Protein
PMC - PMC5407023
OTO - NOTNLM
OT  - calcium imaging
OT  - connexin
OT  - endothelial cell
OT  - mitochondria
OT  - reactive oxygen species
EDAT- 2017/01/27 06:00
MHDA- 2017/06/06 06:00
PMCR- 2018/04/01
CRDT- 2017/01/27 06:00
PHST- 2016/09/27 00:00 [received]
PHST- 2017/01/23 00:00 [revised]
PHST- 2017/01/24 00:00 [accepted]
PHST- 2017/01/27 06:00 [pubmed]
PHST- 2017/06/06 06:00 [medline]
PHST- 2017/01/27 06:00 [entrez]
PHST- 2018/04/01 00:00 [pmc-release]
AID - ajpcell.00283.2016 [pii]
AID - C-00283-2016 [pii]
AID - 10.1152/ajpcell.00283.2016 [doi]
PST - ppublish
SO  - Am J Physiol Cell Physiol. 2017 Apr 1;312(4):C398-C406. doi: 
      10.1152/ajpcell.00283.2016. Epub 2017 Jan 25.