PMID- 28123674 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20201001 IS - 1993-0771 (Print) IS - 1993-078X (Electronic) IS - 1993-0771 (Linking) VI - 10 IP - 4 DP - 2016 TI - Karyological study of Ololygon tripui (Lourenco, Nascimento and Pires, 2009), (Anura, Hylidae) with comments on chromosomal traits among populations. PG - 505-516 LID - 10.3897/CompCytogen.v10i4.9176 [doi] AB - To increase the number of cytogenetic characters used in Ololygon tripui systematics, we applied some cytogenetic techniques such as Giemsa, C- and NOR-banding, and fluorescence in situ hybridization (FISH) with 18S rDNA and repetitive microsatellite DNA probes to the study of four populations from Minas Gerais State (southeastern Brazil). All populations showed 2n = 24 and FN = 48, and chromosomal formula 8m + 10sm + 6st. Nucleolar organizing regions (NORs) were located on chromosome pair 6 in all populations, although in the Tripui locality additional markings were observed on one homologue of chromosome pair 3. These patterns were partially congruent with results obtained using the 18S rDNA FISH probe. The microsatellites repetitive DNA (GA)(15) and (CAT)(10) probes accumulated predominantly in the terminal region of all chromosomes. Chromosome morphology and Ag-NOR were conserved among populations, a conserved pattern in Ololygon Fitzinger, 1843. Repetitive DNA FISH probes patterns were similar among populations, but they revealed species-specific differences when compared with other species of the genus Ololygon, suggesting that molecular cytogenetics are potentially more informative in karyologically conservative taxa. FAU - Peixoto, Marco Antonio A AU - Peixoto MA AD - Laboratorio de Sistematica Molecular - Beagle, Departamento de Biologia Animal, Universidade Federal de Vicosa, CEP 36570-900, Vicosa, Minas Gerais State, Brazil; Museu de Zoologia Joao Moojen - MZUFV, Departamento de Biologia Animal, Universidade Federal de Vicosa, CEP 36570-900, Vicosa, Minas Gerais State, Brazil. FAU - Oliveira, Marina P C AU - Oliveira MP AD - Laboratorio de Sistematica Molecular - Beagle, Departamento de Biologia Animal, Universidade Federal de Vicosa, CEP 36570-900, Vicosa, Minas Gerais State, Brazil. FAU - Feio, Renato N AU - Feio RN AD - Museu de Zoologia Joao Moojen - MZUFV, Departamento de Biologia Animal, Universidade Federal de Vicosa, CEP 36570-900, Vicosa, Minas Gerais State, Brazil. FAU - Dergam, Jorge A AU - Dergam JA AD - Laboratorio de Sistematica Molecular - Beagle, Departamento de Biologia Animal, Universidade Federal de Vicosa, CEP 36570-900, Vicosa, Minas Gerais State, Brazil. LA - eng PT - Journal Article DEP - 20161010 PL - Bulgaria TA - Comp Cytogenet JT - Comparative cytogenetics JID - 101503807 PMC - PMC5240505 OTO - NOTNLM OT - 18S rDNA OT - Ag-NOR OT - Cytotaxonomy OT - FISH OT - heterochromatic blocks OT - microsatellite DNA probes OT - population cytogenetics EDAT- 2017/01/27 06:00 MHDA- 2017/01/27 06:01 PMCR- 2016/10/10 CRDT- 2017/01/27 06:00 PHST- 2016/05/11 00:00 [received] PHST- 2016/07/30 00:00 [accepted] PHST- 2017/01/27 06:00 [entrez] PHST- 2017/01/27 06:00 [pubmed] PHST- 2017/01/27 06:01 [medline] PHST- 2016/10/10 00:00 [pmc-release] AID - 10.3897/CompCytogen.v10i4.9176 [doi] PST - epublish SO - Comp Cytogenet. 2016 Oct 10;10(4):505-516. doi: 10.3897/CompCytogen.v10i4.9176. eCollection 2016.