PMID- 28123684
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1993-0771 (Print)
IS  - 1993-078X (Electronic)
IS  - 1993-0771 (Linking)
VI  - 10
IP  - 4
DP  - 2016
TI  - Karyotypic features including organizations of the 5S, 45S rDNA loci and 
      telomeres of Scadoxus multiflorus (Amaryllidaceae).
PG  - 637-646
LID - 10.3897/CompCytogen.v10i4.9958 [doi]
AB  - Scadoxus multiflorus Martyn, 1795 is an ornamental plant with brilliantly colored 
      flowers. Even though its chromosomes are rather large, there is no karyotype 
      description reported so far. Therefore, conventional and molecular cytogenetic 
      studies including fluorescence in situ hybridization (FISH) with 45S and 5S rDNA, 
      and human telomere sequence (TTAGGG)(n) probes (Arabidopsis-type telomere probes 
      yielded negative results) were carried out. The chromosome number is as reported 
      previously, 2n = 18. The nine chromosome pairs include two large submetacentric, 
      five large acrocentric, one medium acrocentric, two small metacentric and eight 
      small submetacentric chromosomes. Hybridization sites of the 45S rDNA signals 
      were on the short arm ends of chromosomes #1, #3 and #8, while 5S rDNA signals 
      appeared on the long arm of chromosome 3, in one homologue as a double signal. 
      The telomere signals were restricted to all chromosome ends. Three chromosome 
      pairs could be newly identified, chromosome pair 3 by 5S rDNA and chromosomes #1, 
      #3 and #8 by 45S rDNA loci. In addition to new information about rDNA locations 
      we show that the ends of Scadoxus multiflorus chromosomes harbor human instead of 
      Arabidopsis-type telomere sequences. Overall, the Scadoxus multiflorus karyotype 
      presents chromosomal heteromorphy concerning size, shape and 45S and 5S rDNA 
      positioning. As Scadoxus Rafinesque, 1838 and related species are poorly studied 
      on chromosomal level the here presented data is important for better 
      understanding of evolution in Amaryllidaceae.
FAU - Monkheang, Pansa
AU  - Monkheang P
AD  - Department of Biology, Faculty of Science, Khon Kaen University, Khon Kaen 40002, 
      Thailand; Genetics and Environmental Toxicology Research Group, Khon Kaen 
      University, Khon Kaen 40002, Thailand.
FAU - Chaveerach, Arunrat
AU  - Chaveerach A
AD  - Department of Biology, Faculty of Science, Khon Kaen University, Khon Kaen 40002, 
      Thailand; Genetics and Environmental Toxicology Research Group, Khon Kaen 
      University, Khon Kaen 40002, Thailand.
FAU - Sudmoon, Runglawan
AU  - Sudmoon R
AD  - Genetics and Environmental Toxicology Research Group, Khon Kaen University, Khon 
      Kaen 40002, Thailand; Faculty of Law, Khon Kaen University, Khon Kaen 40002, 
      Thailand.
FAU - Tanee, Tawatchai
AU  - Tanee T
AD  - Genetics and Environmental Toxicology Research Group, Khon Kaen University, Khon 
      Kaen 40002, Thailand; Faculty of Environment and Resource Studies, Mahasarakham 
      University, Maha Sarakham 44150, Thailand.
LA  - eng
PT  - Journal Article
DEP - 20161122
PL  - Bulgaria
TA  - Comp Cytogenet
JT  - Comparative cytogenetics
JID - 101503807
PMC - PMC5237775
OTO - NOTNLM
OT  - Scadoxus multiflorus
OT  - aceto-orcein staining
OT  - fluorescence in situ hybridization (FISH)
OT  - rDNA
OT  - telomere
EDAT- 2017/01/27 06:00
MHDA- 2017/01/27 06:01
PMCR- 2016/11/22
CRDT- 2017/01/27 06:00
PHST- 2016/07/20 00:00 [received]
PHST- 2016/10/07 00:00 [accepted]
PHST- 2017/01/27 06:00 [entrez]
PHST- 2017/01/27 06:00 [pubmed]
PHST- 2017/01/27 06:01 [medline]
PHST- 2016/11/22 00:00 [pmc-release]
AID - 10.3897/CompCytogen.v10i4.9958 [doi]
PST - epublish
SO  - Comp Cytogenet. 2016 Nov 22;10(4):637-646. doi: 10.3897/CompCytogen.v10i4.9958. 
      eCollection 2016.