PMID- 28128649
OWN - NLM
STAT- MEDLINE
DCOM- 20171016
LR  - 20180123
IS  - 1556-7125 (Electronic)
IS  - 1535-3141 (Linking)
VI  - 14
IP  - 4
DP  - 2017 Apr
TI  - Effect of Vancomycin, Tylosin, and Chlortetracycline on Vancomycin-Resistant 
      Enterococcus faecium Colonization of Broiler Chickens During Grow-Out.
PG  - 231-237
LID - 10.1089/fpd.2016.2217 [doi]
AB  - Broiler chickens may serve as reservoirs for human colonization by 
      vancomycin-resistant Enterococcus (VRE). We examined the effects of vancomycin 
      and two commonly used antimicrobial feed additives on VRE colonization in broiler 
      chickens during grow-out. Chicks received unsupplemented feed or feed containing 
      vancomycin, chlortetracycline, or tylosin from day of hatch to grow-out at 6 
      weeks. At 3 days of age, chicks received by crop gavage 10(7) colony-forming 
      units (CFUs) of a human or poultry VRE isolate. Cecal contents were monitored 
      weekly for VRE, short-chain fatty acids (SCFAs), and bacterial denaturing 
      gradient gel electrophoresis (DGGE) profile methods. Vancomycin promoted 
      persistent and high-level colonization with human- and poultry-derived VRE to 
      grow-out in comparison with controls, while treatment with chlortetracycline and 
      tylosin did not. Colonization by the poultry isolate in control, 
      chlortetracycline, and tylosin groups persisted throughout the grow-out period 
      with low concentrations present at 6 weeks, whereas the human isolate decreased 
      to an undetectable level by week 6. Vancomycin resulted in significant reductions 
      in cecal acetic acid and butyric acid in comparison with controls, but 
      chlortetracycline and tylosin did not. DGGE profiles contained two main clusters 
      with all vancomycin profiles in a smaller cluster and all other profiles in a 
      larger cluster. These results demonstrate that vancomycin, but not 
      chlortetracycline or tylosin, disrupted the indigenous microbiota and SCFA 
      patterns of broiler chickens and promoted colonization by VRE.
FAU - Hume, Michael E
AU  - Hume ME
AD  - 1 Food and Feed Safety Research Unit, Southern Plains Agricultural Research 
      Center , Agricultural Research Service, U.S. Department of Agriculture, College 
      Station, Texas.
FAU - Donskey, Curtis J
AU  - Donskey CJ
AD  - 2 Infectious Diseases Section, Louis Stokes Cleveland Veterans Affairs Medical 
      Center , Cleveland, Ohio.
LA  - eng
PT  - Journal Article
DEP - 20170127
PL  - United States
TA  - Foodborne Pathog Dis
JT  - Foodborne pathogens and disease
JID - 101120121
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Fatty Acids, Volatile)
RN  - 6Q205EH1VU (Vancomycin)
RN  - WCK1KIQ23Q (Chlortetracycline)
RN  - YEF4JXN031 (Tylosin)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/pharmacology
MH  - Cecum/microbiology
MH  - Chickens/*microbiology
MH  - Chlortetracycline/*pharmacology
MH  - Colony Count, Microbial
MH  - Denaturing Gradient Gel Electrophoresis
MH  - *Drug Resistance, Multiple, Bacterial
MH  - Enterococcus faecium/*drug effects/isolation & purification
MH  - Fatty Acids, Volatile/analysis
MH  - Feces/microbiology
MH  - Food Contamination/analysis
MH  - Food Microbiology
MH  - Poultry/microbiology
MH  - Tylosin/*pharmacology
MH  - Vancomycin/*pharmacology
OTO - NOTNLM
OT  - DGGE
OT  - SCFA
OT  - VRE
OT  - broiler
OT  - ceca
EDAT- 2017/01/28 06:00
MHDA- 2017/10/17 06:00
CRDT- 2017/01/28 06:00
PHST- 2017/01/28 06:00 [pubmed]
PHST- 2017/10/17 06:00 [medline]
PHST- 2017/01/28 06:00 [entrez]
AID - 10.1089/fpd.2016.2217 [doi]
PST - ppublish
SO  - Foodborne Pathog Dis. 2017 Apr;14(4):231-237. doi: 10.1089/fpd.2016.2217. Epub 
      2017 Jan 27.