PMID- 28128898
OWN - NLM
STAT- MEDLINE
DCOM- 20171201
LR  - 20171201
IS  - 2192-2659 (Electronic)
IS  - 2192-2640 (Linking)
VI  - 6
IP  - 7
DP  - 2017 Apr
TI  - Tri-Layered Nanocomposite Hydrogel Scaffold for the Concurrent Regeneration of 
      Cementum, Periodontal Ligament, and Alveolar Bone.
LID - 10.1002/adhm.201601251 [doi]
AB  - A tri-layered scaffolding approach is adopted for the complete and concurrent 
      regeneration of hard tissues-cementum and alveolar bone-and soft tissue-the 
      periodontal ligament (PDL)-at a periodontal defect site. The porous tri-layered 
      nanocomposite hydrogel scaffold is composed of chitin-poly(lactic-co-glycolic 
      acid) (PLGA)/nanobioactive glass ceramic (nBGC)/cementum protein 1 as the 
      cementum layer, chitin-PLGA/fibroblast growth factor 2 as the PDL layer, and 
      chitin-PLGA/nBGC/platelet-rich plasma derived growth factors as the alveolar bone 
      layer. The tri-layered nanocomposite hydrogel scaffold is cytocompatible and 
      favored cementogenic, fibrogenic, and osteogenic differentiation of human dental 
      follicle stem cells. In vivo, tri-layered nanocomposite hydrogel scaffold 
      with/without growth factors is implanted into rabbit maxillary periodontal 
      defects and compared with the controls at 1 and 3 months postoperatively. The 
      tri-layered nanocomposite hydrogel scaffold with growth factors demonstrates 
      complete defect closure and healing with new cancellous-like tissue formation on 
      microcomputed tomography analysis. Histological and immunohistochemical analyses 
      further confirm the formation of new cementum, fibrous PDL, and alveolar bone 
      with well-defined bony trabeculae in comparison to the other three groups. In 
      conclusion, the tri-layered nanocomposite hydrogel scaffold with growth factors 
      can serve as an alternative regenerative approach to achieve simultaneous and 
      complete periodontal regeneration.
CI  - (c) 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Sowmya, S
AU  - Sowmya S
AD  - Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of 
      Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham, Amrita 
      University, 682041, Kochi, India.
FAU - Mony, Ullas
AU  - Mony U
AD  - Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of 
      Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham, Amrita 
      University, 682041, Kochi, India.
FAU - Jayachandran, P
AU  - Jayachandran P
AD  - Amrita School of Dentistry, Amrita Institute of Medical Sciences and Research 
      Centre, Amrita Vishwa Vidyapeetham, Amrita University, 682041, Kochi, India.
FAU - Reshma, S
AU  - Reshma S
AD  - Amrita School of Dentistry, Amrita Institute of Medical Sciences and Research 
      Centre, Amrita Vishwa Vidyapeetham, Amrita University, 682041, Kochi, India.
FAU - Kumar, R Arun
AU  - Kumar RA
AD  - Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of 
      Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham, Amrita 
      University, 682041, Kochi, India.
FAU - Arzate, H
AU  - Arzate H
AD  - Laboratorio de Biologia Periodontal, Facultad de Odontologia, Universidad 
      Nacional Autonoma de Mexico, D.F 04510, Mexico City, Mexico.
FAU - Nair, Shantikumar V
AU  - Nair SV
AD  - Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of 
      Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham, Amrita 
      University, 682041, Kochi, India.
FAU - Jayakumar, R
AU  - Jayakumar R
AD  - Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of 
      Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham, Amrita 
      University, 682041, Kochi, India.
LA  - eng
PT  - Journal Article
DEP - 20170127
PL  - Germany
TA  - Adv Healthc Mater
JT  - Advanced healthcare materials
JID - 101581613
RN  - 0 (Hydrogels)
SB  - IM
MH  - *Alveolar Bone Loss/metabolism/pathology/therapy
MH  - Animals
MH  - Bone Regeneration/*drug effects
MH  - *Dental Cementum/injuries/metabolism/pathology
MH  - *Hydrogels/chemistry/pharmacology
MH  - *Nanocomposites/chemistry/therapeutic use
MH  - *Periodontal Ligament/injuries/metabolism/pathology
MH  - Rabbits
MH  - Tissue Scaffolds/*chemistry
OTO - NOTNLM
OT  - alveolar bone
OT  - cementum
OT  - layered
OT  - nanocomposite
OT  - periodontal ligament
OT  - scaffold
EDAT- 2017/01/28 06:00
MHDA- 2017/12/02 06:00
CRDT- 2017/01/28 06:00
PHST- 2016/11/08 00:00 [received]
PHST- 2016/12/19 00:00 [revised]
PHST- 2017/01/28 06:00 [pubmed]
PHST- 2017/12/02 06:00 [medline]
PHST- 2017/01/28 06:00 [entrez]
AID - 10.1002/adhm.201601251 [doi]
PST - ppublish
SO  - Adv Healthc Mater. 2017 Apr;6(7). doi: 10.1002/adhm.201601251. Epub 2017 Jan 27.