PMID- 28129707
OWN - NLM
STAT- MEDLINE
DCOM- 20170315
LR  - 20220408
IS  - 1549-7828 (Electronic)
IS  - 1040-841X (Linking)
VI  - 43
IP  - 3
DP  - 2017 May
TI  - Fluorescence in situ hybridization (FISH) in the microbiological diagnostic 
      routine laboratory: a review.
PG  - 263-293
LID - 10.3109/1040841X.2016.1169990 [doi]
AB  - Early identification of microbial pathogens is essential for rational and 
      conservative antibiotic use especially in the case of known regional resistance 
      patterns. Here, we describe fluorescence in situ hybridization (FISH) as one of 
      the rapid methods for easy identification of microbial pathogens, and its 
      advantages and disadvantages for the diagnosis of pathogens in human infections 
      in the laboratory diagnostic routine. Binding of short fluorescence-labeled DNA 
      or nucleic acid-mimicking PNA probes to ribosomes of infectious agents with 
      consecutive analysis by fluorescence microscopy allows identification of 
      bacterial and eukaryotic pathogens at genus or species level. FISH analysis leads 
      to immediate differentiation of infectious agents without delay due to the need 
      for microbial culture. As a microscopic technique, FISH has the unique potential 
      to provide information about spatial resolution, morphology and identification of 
      key pathogens in mixed species samples. On-going automation and commercialization 
      of the FISH procedure has led to significant shortening of the time-to-result and 
      increased test reliability. FISH is a useful tool for the rapid initial 
      identification of microbial pathogens, even from primary materials. Among the 
      rapidly developing alternative techniques, FISH serves as a bridging technology 
      between microscopy, microbial culture, biochemical identification and molecular 
      diagnostic procedures.
FAU - Frickmann, Hagen
AU  - Frickmann H
AD  - a German Armed Forces Hospital of Hamburg, Department of Tropical Medicine at the 
      Bernhard Nocht Institute , Hamburg , Germany.
FAU - Zautner, Andreas Erich
AU  - Zautner AE
AD  - b Department of Medical Microbiology, University Medical Center Gottingen , 
      Gottingen , Germany.
FAU - Moter, Annette
AU  - Moter A
AD  - c University Medical Center Berlin, Biofilmcenter at the German Heart Institute 
      Berlin , Berlin , Germany.
FAU - Kikhney, Judith
AU  - Kikhney J
AD  - c University Medical Center Berlin, Biofilmcenter at the German Heart Institute 
      Berlin , Berlin , Germany.
FAU - Hagen, Ralf Matthias
AU  - Hagen RM
AD  - a German Armed Forces Hospital of Hamburg, Department of Tropical Medicine at the 
      Bernhard Nocht Institute , Hamburg , Germany.
FAU - Stender, Henrik
AU  - Stender H
AD  - d Stender Diagnostics , Gentofte , Denmark.
FAU - Poppert, Sven
AU  - Poppert S
AD  - e Institute for Medical Microbiology, Justus-Liebig-University Giessen , Giessen 
      , Germany.
LA  - eng
PT  - Journal Article
PT  - Review
DEP - 20170127
PL  - England
TA  - Crit Rev Microbiol
JT  - Critical reviews in microbiology
JID - 8914274
RN  - 0 (Fluorescent Dyes)
SB  - IM
MH  - Bacteria/*genetics/pathogenicity
MH  - Bacterial Infections/*microbiology
MH  - Biofilms
MH  - Fluorescent Dyes
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Microbiological Techniques/*methods
MH  - Mouth Diseases/microbiology
MH  - Mycobacterium/genetics/pathogenicity
MH  - Permeability
MH  - Respiratory Tract Infections/diagnosis/microbiology
MH  - Tissue Fixation
MH  - Virus Diseases/virology
MH  - Wound Infection/microbiology
OTO - NOTNLM
OT  - Fluorescence in situ hybridization
OT  - rapid diagnostics of infectious diseases
EDAT- 2017/01/29 06:00
MHDA- 2017/03/16 06:00
CRDT- 2017/01/29 06:00
PHST- 2017/01/29 06:00 [pubmed]
PHST- 2017/03/16 06:00 [medline]
PHST- 2017/01/29 06:00 [entrez]
AID - 10.3109/1040841X.2016.1169990 [doi]
PST - ppublish
SO  - Crit Rev Microbiol. 2017 May;43(3):263-293. doi: 10.3109/1040841X.2016.1169990. 
      Epub 2017 Jan 27.