PMID- 28129792
OWN - NLM
STAT- MEDLINE
DCOM- 20171108
LR  - 20220410
IS  - 1757-6512 (Electronic)
IS  - 1757-6512 (Linking)
VI  - 8
IP  - 1
DP  - 2017 Jan 28
TI  - Intramuscular injection of human umbilical cord-derived mesenchymal stem cells 
      improves cardiac function in dilated cardiomyopathy rats.
PG  - 18
LID - 10.1186/s13287-017-0472-y [doi]
LID - 18
AB  - BACKGROUND: Stem cells provide a promising candidate for the treatment of the 
      fatal pediatric dilated cardiomyopathy (DCM). This study aimed to investigate the 
      effects of intramuscular injection of human umbilical cord-derived mesenchymal 
      stem cells (hUCMSCs) on the cardiac function of a DCM rat model. METHODS: A DCM 
      model was established by intraperitoneal injections of doxorubicin in 
      Sprague-Dawley rats. hUCMSCs at different concentrations or cultured medium were 
      injected via limb skeletal muscles, with blank medium injected as the control. 
      The rats were monitored for 4 weeks, meanwhile BNP, cTNI, VEGF, HGF, GM-CSF, and 
      LIF in the peripheral blood were examined by ELISA, and cardiac function was 
      monitored by echocardiography (Echo-CG). Finally, the expression of IGF-1, HGF, 
      and VEGF in the myocardium was examined by histoimmunochemistry and real-time 
      PCR, and the ultrastructure of the myocardium was examined by electron 
      microscopy. RESULTS: Injection of hUCMSCs markedly improved cardiac function in 
      the DCM rats by significantly elevating left ventricular ejection fraction (LVEF) 
      and left ventricular fraction shortening (LVFS). The BNP and cTNI levels in the 
      peripheral blood were reduced by hUCMSCs, while HGF, LIF, GM-CSF, and VEGF were 
      increased by hUCMSCs. Expression of IGF-1, HGF, and VEGF in the myocardium from 
      the DCM rats was significantly increased by hUCMSC injection. Furthermore, 
      hUCMSCs protected the ultrastructure of cardiomyocytes by attenuating 
      mitochondrial swelling and maintaining sarcolemma integrity. CONCLUSIONS: 
      Intramuscular injection of UCMSCs can improve DCM-induced cardiac function 
      impairment and protect the myocardium. These effects may be mediated by 
      regulation of relevant cytokines in serum and the myocardium.
FAU - Mao, Chenggang
AU  - Mao C
AD  - Department of Pediatrics, the Affiliated Hospital of Qingdao University, 16 
      Jiangsu Rd, Qingdao, 266003, China.
FAU - Hou, Xu
AU  - Hou X
AD  - Department of Endocrinology, the Affiliated Hospital of Qingdao University, 
      Qingdao, China.
AD  - The Institute of Metabolic Diseases, Qingdao University, Qingdao, China.
FAU - Wang, Benzhen
AU  - Wang B
AD  - Department of Pediatrics, Qingdao Women and Children's Hospital, 6 Tongfu Rd, 
      Qingdao, 266011, China.
FAU - Chi, Jingwei
AU  - Chi J
AD  - Department of Endocrinology, the Affiliated Hospital of Qingdao University, 
      Qingdao, China.
FAU - Jiang, Yanjie
AU  - Jiang Y
AD  - Department of Pediatrics, the Affiliated Hospital of Qingdao University, 16 
      Jiangsu Rd, Qingdao, 266003, China.
FAU - Zhang, Caining
AU  - Zhang C
AD  - Department of Pediatrics, the Affiliated Hospital of Qingdao University, 16 
      Jiangsu Rd, Qingdao, 266003, China.
FAU - Li, Zipu
AU  - Li Z
AUID- ORCID: 0000-0002-5244-272X
AD  - Department of Pediatrics, the Affiliated Hospital of Qingdao University, 16 
      Jiangsu Rd, Qingdao, 266003, China. zipuli2016@sina.com.
AD  - Department of Pediatrics, Qingdao Women and Children's Hospital, 6 Tongfu Rd, 
      Qingdao, 266011, China. zipuli2016@sina.com.
LA  - eng
PT  - Journal Article
DEP - 20170128
PL  - England
TA  - Stem Cell Res Ther
JT  - Stem cell research & therapy
JID - 101527581
RN  - 0 (Leukemia Inhibitory Factor)
RN  - 0 (Troponin I)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (vascular endothelial growth factor A, rat)
RN  - 114471-18-0 (Natriuretic Peptide, Brain)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - Cardiomyopathy, Dilated/genetics/metabolism/pathology/*therapy
MH  - Fetal Blood/cytology/metabolism
MH  - Gene Expression Regulation
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/blood/genetics
MH  - Hepatocyte Growth Factor/blood/genetics
MH  - Humans
MH  - Injections, Intramuscular
MH  - Insulin-Like Growth Factor I/genetics/metabolism
MH  - Leukemia Inhibitory Factor/blood/genetics
MH  - Male
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/cytology/*metabolism
MH  - Mitochondria, Heart/metabolism/pathology
MH  - Myocardium/metabolism/pathology
MH  - Natriuretic Peptide, Brain/blood/genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Recovery of Function/*physiology
MH  - Stroke Volume/*physiology
MH  - Transplantation, Heterologous
MH  - Troponin I/blood/genetics
MH  - Vascular Endothelial Growth Factor A/blood/genetics
MH  - Ventricular Function, Left/*physiology
PMC - PMC5273808
OTO - NOTNLM
OT  - Cardiac function
OT  - Dilated cardiomyopathy (DCM)
OT  - Intramuscular injection
OT  - Mesenchymal stem cells (MSCs)
OT  - Paracrine
EDAT- 2017/01/29 06:00
MHDA- 2017/11/09 06:00
PMCR- 2017/01/28
CRDT- 2017/01/29 06:00
PHST- 2016/11/24 00:00 [received]
PHST- 2017/01/06 00:00 [accepted]
PHST- 2016/12/22 00:00 [revised]
PHST- 2017/01/29 06:00 [entrez]
PHST- 2017/01/29 06:00 [pubmed]
PHST- 2017/11/09 06:00 [medline]
PHST- 2017/01/28 00:00 [pmc-release]
AID - 10.1186/s13287-017-0472-y [pii]
AID - 472 [pii]
AID - 10.1186/s13287-017-0472-y [doi]
PST - epublish
SO  - Stem Cell Res Ther. 2017 Jan 28;8(1):18. doi: 10.1186/s13287-017-0472-y.