PMID- 28130157
OWN - NLM
STAT- MEDLINE
DCOM- 20171019
LR  - 20180917
IS  - 0304-4165 (Print)
IS  - 0304-4165 (Linking)
VI  - 1861
IP  - 4
DP  - 2017 Apr
TI  - Acridine orange interaction with DNA: Effect of ionic strength.
PG  - 900-909
LID - S0304-4165(17)30031-4 [pii]
LID - 10.1016/j.bbagen.2017.01.023 [doi]
AB  - BACKGROUND: The study of acridine orange (AO) spectral characteristics and the 
      quenching of its singlet and triplet excited states by TEMPO radical at its 
      binding to DNA in the function of the DNA concentration and in the absence and 
      presence of NaCl is reported. METHODS: The study was performed using steady-state 
      and time resolved optical absorption and florescence, fluorescence correlation 
      spectroscopy and resonant light scattering techniques. RESULTS: The presence of 
      different species in equilibrium: AO monomers and aggregates bound to DNA, has 
      been demonstrated, their relative content depending on the DNA and the AO 
      concentrations. At high DNA concentration the AO monomers are protected against 
      the contact with other molecules, thus reducing the AO excited state quenching. 
      The addition of NaCl reduces the AO binding constant to DNA, thus reducing the AO 
      and DNA aggregation. CONCLUSIONS: The interaction of AO with DNA is a complex 
      process, including aggregation and disaggregation of both components. This 
      modifies the AO excited state characteristics and AO accessibility to other 
      molecules. The salt reduces the DNA effects on the AO excited state 
      characteristics thus attenuating its effects on the AO efficacy in applications. 
      GENERAL SIGNIFICANCE: This study demonstrates that the interaction of 
      photosensitizers with DNA, depending on their relative concentrations, can both 
      decrease and increase the photosensitizer efficacy in applications. The salt is 
      able to attenuate these effects.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Amado, Andre M
AU  - Amado AM
AD  - Departamento de Fisica, Faculdade de Filosofia Ciencias e Letras de Ribeirao 
      Preto, Universidade de Sao Paulo, Av. Bandeirantes 3900, Vila Monte Alegre, CEP 
      14040-901 Ribeirao Preto, SP, Brazil.
FAU - Pazin, Wallance M
AU  - Pazin WM
AD  - Departamento de Fisica, Faculdade de Filosofia Ciencias e Letras de Ribeirao 
      Preto, Universidade de Sao Paulo, Av. Bandeirantes 3900, Vila Monte Alegre, CEP 
      14040-901 Ribeirao Preto, SP, Brazil.
FAU - Ito, Amando S
AU  - Ito AS
AD  - Departamento de Fisica, Faculdade de Filosofia Ciencias e Letras de Ribeirao 
      Preto, Universidade de Sao Paulo, Av. Bandeirantes 3900, Vila Monte Alegre, CEP 
      14040-901 Ribeirao Preto, SP, Brazil.
FAU - Kuzmin, Vladimir A
AU  - Kuzmin VA
AD  - Emanuel Institute of Biochemical Physics, Russian Academy of Science, Kosygin st. 
      4, 117977 Moscow, Russia.
FAU - Borissevitch, Iouri E
AU  - Borissevitch IE
AD  - Departamento de Fisica, Faculdade de Filosofia Ciencias e Letras de Ribeirao 
      Preto, Universidade de Sao Paulo, Av. Bandeirantes 3900, Vila Monte Alegre, CEP 
      14040-901 Ribeirao Preto, SP, Brazil. Electronic address: iouribor@usp.br.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170124
PL  - Netherlands
TA  - Biochim Biophys Acta Gen Subj
JT  - Biochimica et biophysica acta. General subjects
JID - 101731726
RN  - 451W47IQ8X (Sodium Chloride)
RN  - 9007-49-2 (DNA)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/*chemistry
MH  - DNA/*chemistry
MH  - Osmolar Concentration
MH  - Sodium Chloride/chemistry
MH  - Spectrometry, Fluorescence/methods
EDAT- 2017/01/29 06:00
MHDA- 2017/10/20 06:00
CRDT- 2017/01/29 06:00
PHST- 2016/07/19 00:00 [received]
PHST- 2016/12/31 00:00 [revised]
PHST- 2017/01/09 00:00 [accepted]
PHST- 2017/01/29 06:00 [pubmed]
PHST- 2017/10/20 06:00 [medline]
PHST- 2017/01/29 06:00 [entrez]
AID - S0304-4165(17)30031-4 [pii]
AID - 10.1016/j.bbagen.2017.01.023 [doi]
PST - ppublish
SO  - Biochim Biophys Acta Gen Subj. 2017 Apr;1861(4):900-909. doi: 
      10.1016/j.bbagen.2017.01.023. Epub 2017 Jan 24.