PMID- 28130912
OWN - NLM
STAT- MEDLINE
DCOM- 20191009
LR  - 20191010
IS  - 1932-7005 (Electronic)
IS  - 1932-6254 (Linking)
VI  - 12
IP  - 2
DP  - 2018 Feb
TI  - Effects on human heart valve immunogenicity in vitro by high concentration 
      cryoprotectant treatment.
PG  - e1046-e1055
LID - 10.1002/term.2426 [doi]
AB  - It has been shown previously that cryopreservation, using an ice-free 
      cryopreservation method with the cryoprotectant formulation VS83, beneficially 
      modulated immune reactions in vivo and in vitro when compared with conventionally 
      frozen tissues. In this study, we assessed the impact of a VS83 post-treatment of 
      previously conventionally frozen human tissue on responses of human immune cells 
      in vitro. Tissue punches of treated and non-treated (control) aortic heart valve 
      tissue (leaflets and associated aortic root) were co-cultured for 7 days with 
      peripheral blood mononuclear cells or enriched CD14(+) monocytes. Effects on 
      cellular activation markers, cytokine secretion and immune cell proliferation 
      were analysed by flow cytometry. Flow cytometry studies showed that VS83 
      treatment of aortic root tissue promoted activation and differentiation of 
      CD14(+) monocytes, inducing both up-regulation of CD16 and down-regulation of 
      CD14. Significantly enhanced expression levels for the C-C chemokine receptor 
      (CCR)7 and the human leukocyte antigen (HLA)-DR on monocytes co-cultured with 
      VS83-treated aortic root tissue were measured, while the interleukin (IL)-6 and 
      monocyte chemoattractant protein (MCP)-1 release was suppressed. However, the 
      levels of interferon (IFN)gamma and tumour necrosis factor (TNF)alpha remained 
      undetectable, indicating that complete activation into pro-inflammatory 
      macrophages did not occur. Similar, but non-significant, changes occurred with 
      VS83-treated leaflets. Additionally, in co-cultures with T cells, proliferation 
      and cytokine secretion responses were minimal. In conclusion, post-treatment of 
      conventionally cryopreserved human heart valve tissue with the VS83 formulation 
      induces changes in the activation and differentiation characteristics of human 
      monocytes, and thereby may influence long-term performance following 
      implantation. Copyright (c) 2017 John Wiley & Sons, Ltd.
CI  - Copyright (c) 2017 John Wiley & Sons, Ltd.
FAU - Hogerle, Benjamin A
AU  - Hogerle BA
AD  - Department for Thoracic and Cardiovascular Surgery, Johann Wolfgang Goethe 
      University, Frankfurt am Main, Germany.
FAU - Schneider, Maria
AU  - Schneider M
AD  - Institute of Medical Immunology, Charite Universitatsmedizin Berlin, Germany.
AD  - Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Charite 
      Universitatsmedizin, Berlin, Germany.
FAU - Sudrow, Katrin
AU  - Sudrow K
AD  - Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Charite 
      Universitatsmedizin, Berlin, Germany.
FAU - Souidi, Naima
AU  - Souidi N
AD  - Institute of Medical Immunology, Charite Universitatsmedizin Berlin, Germany.
AD  - Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Charite 
      Universitatsmedizin, Berlin, Germany.
FAU - Stolk, Meaghan
AU  - Stolk M
AD  - Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Charite 
      Universitatsmedizin, Berlin, Germany.
FAU - Werner, Isabella
AU  - Werner I
AD  - Department for Thoracic and Cardiovascular Surgery, Johann Wolfgang Goethe 
      University, Frankfurt am Main, Germany.
FAU - Biermann, Anna
AU  - Biermann A
AD  - Department for Thoracic and Cardiovascular Surgery, Johann Wolfgang Goethe 
      University, Frankfurt am Main, Germany.
FAU - Brockbank, Kelvin G M
AU  - Brockbank KGM
AD  - Tissue Testing Technologies LLC, North Charleston, SC, USA.
AD  - Department of Bioengineering, Clemson University, SC, USA.
AD  - Department of Regenerative Medicine and Cell Biology, Medical University of South 
      Carolina, Charleston, SC, USA.
FAU - Stock, Ulrich A
AU  - Stock UA
AD  - Department for Thoracic and Cardiovascular Surgery, Johann Wolfgang Goethe 
      University, Frankfurt am Main, Germany.
FAU - Seifert, Martina
AU  - Seifert M
AUID- ORCID: 0000-0002-6372-9769
AD  - Institute of Medical Immunology, Charite Universitatsmedizin Berlin, Germany.
AD  - Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Charite 
      Universitatsmedizin, Berlin, Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170607
PL  - England
TA  - J Tissue Eng Regen Med
JT  - Journal of tissue engineering and regenerative medicine
JID - 101308490
RN  - 0 (Cryoprotective Agents)
RN  - 0 (Cytokines)
RN  - 0 (Quinazolines)
RN  - 0 (Thiones)
RN  - 0 (vasastrol VS-83)
SB  - IM
MH  - Cell Differentiation/drug effects
MH  - Cell Proliferation/drug effects
MH  - Cryopreservation
MH  - Cryoprotective Agents/*pharmacology
MH  - Cytokines/metabolism
MH  - Freezing
MH  - Heart Valves/drug effects/*immunology
MH  - Humans
MH  - Macrophages/drug effects/metabolism
MH  - Monocytes/cytology/drug effects
MH  - Quinazolines/pharmacology
MH  - Thiones/pharmacology
OTO - NOTNLM
OT  - cryopreservation
OT  - heart valve
OT  - human aorta
OT  - immunogenicity
EDAT- 2017/01/29 06:00
MHDA- 2019/10/11 06:00
CRDT- 2017/01/29 06:00
PHST- 2016/06/24 00:00 [received]
PHST- 2017/01/16 00:00 [revised]
PHST- 2017/01/20 00:00 [accepted]
PHST- 2017/01/29 06:00 [pubmed]
PHST- 2019/10/11 06:00 [medline]
PHST- 2017/01/29 06:00 [entrez]
AID - 10.1002/term.2426 [doi]
PST - ppublish
SO  - J Tissue Eng Regen Med. 2018 Feb;12(2):e1046-e1055. doi: 10.1002/term.2426. Epub 
      2017 Jun 7.