PMID- 28131957
OWN - NLM
STAT- MEDLINE
DCOM- 20171122
LR  - 20180329
IS  - 1876-7753 (Electronic)
IS  - 1873-5061 (Linking)
VI  - 19
DP  - 2017 Mar
TI  - The role of methylation, DNA polymorphisms and microRNAs on HLA-G expression in 
      human embryonic stem cells.
PG  - 118-127
LID - S1873-5061(17)30005-3 [pii]
LID - 10.1016/j.scr.2017.01.005 [doi]
AB  - The human leukocyte antigen (HLA)-G gene seems to play a pivotal role in maternal 
      tolerance to the fetus. Little is known about HLA-G expression and its molecular 
      control during in vivo human embryogenesis. Human embryonic stem cells (hESC) 
      provide an interesting in vitro model to study early human development. Different 
      studies reported discrepant findings on whether HLA-G mRNA and protein are 
      present or absent in hESC. Several lines of evidence indicate that promoter CpG 
      methylation and 3' untranslated region (3'UTR) polymorphisms may influence HLA-G 
      expression. We investigated how HLA-G expression is linked to the patterns of 
      promoter methylation and explored the role of the 3'UTR polymorphic sites and 
      their binding microRNAs on the post-transcriptional regulation of HLA-G in eight 
      hESC lines. We showed that, while the gross expression levels of HLA-G are 
      controlled by promoter methylation, the genetic constitution of the HLA-G 3'UTR, 
      more specifically the 14bp insertion in combination with the +3187A/A and 
      +3142G/G SNP, plays a major role in HLA-G mRNA regulation in hESC. Our findings 
      provide a solid first step towards future work using hESC as tools for the study 
      of early human developmental processes in normal and pregnancy-related disorders 
      such as preeclampsia.
CI  - Copyright (c) 2017 The Authors. Published by Elsevier B.V. All rights reserved.
FAU - Verloes, A
AU  - Verloes A
AD  - Department of Hematology, Universitair Ziekenhuis Brussel (UZ Brussel), 1090 
      Brussels, Belgium; Research Group Reproductive Immunology and Implantation 
      (REIM), Vrije Universiteit Brussel (VUB), 1090 Brussels, Belgium. Electronic 
      address: an.verloes@vub.ac.be.
FAU - Spits, C
AU  - Spits C
AD  - Research Group Reproduction and Genetics (REGE), Vrije Universiteit Brussel 
      (VUB), 1090 Brussels, Belgium.
FAU - Vercammen, M
AU  - Vercammen M
AD  - Department of Hematology, Universitair Ziekenhuis Brussel (UZ Brussel), 1090 
      Brussels, Belgium; Research Group Reproductive Immunology and Implantation 
      (REIM), Vrije Universiteit Brussel (VUB), 1090 Brussels, Belgium.
FAU - Geens, M
AU  - Geens M
AD  - Research Group Reproduction and Genetics (REGE), Vrije Universiteit Brussel 
      (VUB), 1090 Brussels, Belgium.
FAU - LeMaoult, J
AU  - LeMaoult J
AD  - Service de Recherches en Hemato-Immunologie, Hopital Saint-Louis, 75475 Paris, 
      France.
FAU - Sermon, K
AU  - Sermon K
AD  - Research Group Reproduction and Genetics (REGE), Vrije Universiteit Brussel 
      (VUB), 1090 Brussels, Belgium.
FAU - Coucke, W
AU  - Coucke W
AD  - Department of Quality of Medical Laboratories, Scientific Institute of Public 
      Health, 1050 Brussels, Belgium.
FAU - Van de Velde, H
AU  - Van de Velde H
AD  - Research Group Reproductive Immunology and Implantation (REIM), Vrije 
      Universiteit Brussel (VUB), 1090 Brussels, Belgium; Research Group Reproduction 
      and Genetics (REGE), Vrije Universiteit Brussel (VUB), 1090 Brussels, Belgium; 
      Center for Reproductive Medicine, Universitair Ziekenhuis Brussel (UZ Brussel), 
      1090 Brussels, Belgium.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170109
PL  - England
TA  - Stem Cell Res
JT  - Stem cell research
JID - 101316957
RN  - 0 (3' Untranslated Regions)
RN  - 0 (HLA-G Antigens)
RN  - 0 (MicroRNAs)
RN  - 0 (Protein Isoforms)
RN  - 0 (RNA, Messenger)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - 3' Untranslated Regions
MH  - Alleles
MH  - Cell Line
MH  - DNA/chemistry/genetics/*metabolism
MH  - DNA Methylation
MH  - Gene Frequency
MH  - Genotype
MH  - HLA-G Antigens/genetics/*metabolism
MH  - Human Embryonic Stem Cells/cytology/*metabolism
MH  - Humans
MH  - MicroRNAs/genetics/*metabolism
MH  - Polymorphism, Single Nucleotide
MH  - Promoter Regions, Genetic
MH  - Protein Isoforms/genetics/metabolism
MH  - RNA, Messenger/metabolism
MH  - Sequence Analysis, DNA
OTO - NOTNLM
OT  - 3'UTR
OT  - HLA-G
OT  - MicroRNA
OT  - Promoter methylation
OT  - hESC
EDAT- 2017/01/31 06:00
MHDA- 2017/11/29 06:00
CRDT- 2017/01/30 06:00
PHST- 2016/06/02 00:00 [received]
PHST- 2016/11/29 00:00 [revised]
PHST- 2017/01/04 00:00 [accepted]
PHST- 2017/01/31 06:00 [pubmed]
PHST- 2017/11/29 06:00 [medline]
PHST- 2017/01/30 06:00 [entrez]
AID - S1873-5061(17)30005-3 [pii]
AID - 10.1016/j.scr.2017.01.005 [doi]
PST - ppublish
SO  - Stem Cell Res. 2017 Mar;19:118-127. doi: 10.1016/j.scr.2017.01.005. Epub 2017 Jan 
      9.