PMID- 28132711
OWN - NLM
STAT- MEDLINE
DCOM- 20180108
LR  - 20220317
IS  - 1878-3554 (Electronic)
IS  - 0099-2399 (Linking)
VI  - 43
IP  - 2
DP  - 2017 Feb
TI  - Nephronectin Stimulates the Differentiation of MDPC-23 Cells into an 
      Odontoblast-like Phenotype.
PG  - 263-271
LID - S0099-2399(16)30791-9 [pii]
LID - 10.1016/j.joen.2016.10.028 [doi]
AB  - INTRODUCTION: The present study investigated the in vitro effects of nephronectin 
      (Npnt) on the proliferation, differentiation, and mineralization of a rat 
      odontoblast-like cell line (MDPC-23 cells). METHODS: MDPC-23 cells were cultured 
      on Npnt-coated polystyrene or in the presence of soluble Npnt. Cell proliferation 
      was analyzed using a Cell Counting Kit-8 kit (Dojindo, Kumamoto, Japan). Alkaline 
      phosphatase (ALP) activity was quantified using an ALP activity assay. A 
      reverse-transcription polymerase chain reaction was performed to evaluate the 
      messenger RNA (mRNA) expression level of odontogenic markers and integrin(s). 
      Alizarin red staining was conducted to quantify the calcium deposition. RESULTS: 
      Soluble Npnt had no adverse effect on the proliferation of MDPC-23 cells, but it 
      exhibited concentration-dependent inhibitory activity toward differentiation. In 
      contrast, coated Npnt promoted cell proliferation dramatically and significantly 
      up-regulated the mRNA expression of odontogenesis-related genes; moreover, mRNA 
      expression of integrin alpha1, alpha3, alpha5, beta1, and beta5 was found to be augmented. 
      MDPC-23 cells cultured on Npnt-coated polystyrene displayed markedly higher ALP 
      activity as early as day 3 after inoculation. In addition, mineralization was 
      accelerated on Npnt-coated polystyrene. CONCLUSIONS: Npnt in its immobilized form 
      enhanced the proliferation of MDPC-23 cells and induced this odontoblastic 
      precursor cell line to differentiate into a mineralizing phenotype.
CI  - Copyright (c) 2016 American Association of Endodontists. Published by Elsevier Inc. 
      All rights reserved.
FAU - Tang, Jia
AU  - Tang J
AD  - Division of Clinical Cariology, Department of Oral Rehabilitation, School of 
      Dentistry, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Hokkaido, 
      Japan. Electronic address: tangjia@hoku-iryo-u.ac.jp.
FAU - Saito, Takashi
AU  - Saito T
AD  - Division of Clinical Cariology, Department of Oral Rehabilitation, School of 
      Dentistry, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Hokkaido, 
      Japan.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Endod
JT  - Journal of endodontics
JID - 7511484
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Polystyrenes)
RN  - 0 (nephronectin)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
MH  - Alkaline Phosphatase/metabolism
MH  - Animals
MH  - Cell Differentiation/*drug effects
MH  - Cell Line
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects
MH  - Extracellular Matrix Proteins/*pharmacology
MH  - Odontoblasts/*drug effects
MH  - Phenotype
MH  - Polystyrenes
MH  - Rats
OTO - NOTNLM
OT  - Dentin
OT  - differentiation
OT  - nephronectin
OT  - odontoblast
EDAT- 2017/01/31 06:00
MHDA- 2018/01/09 06:00
CRDT- 2017/01/31 06:00
PHST- 2016/04/01 00:00 [received]
PHST- 2016/10/18 00:00 [revised]
PHST- 2016/10/22 00:00 [accepted]
PHST- 2017/01/31 06:00 [entrez]
PHST- 2017/01/31 06:00 [pubmed]
PHST- 2018/01/09 06:00 [medline]
AID - S0099-2399(16)30791-9 [pii]
AID - 10.1016/j.joen.2016.10.028 [doi]
PST - ppublish
SO  - J Endod. 2017 Feb;43(2):263-271. doi: 10.1016/j.joen.2016.10.028.