PMID- 28134325
OWN - NLM
STAT- MEDLINE
DCOM- 20181024
LR  - 20181113
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 7
DP  - 2017 Jan 30
TI  - Basophil-derived IL-6 regulates T(H)17 cell differentiation and CD4 T cell 
      immunity.
PG  - 41744
LID - 10.1038/srep41744 [doi]
LID - 41744
AB  - Basophils are rare, circulating granulocytes proposed to be involved in T helper 
      (T(H)) type 2 immunity, mainly through secretion of interleukin (IL)-4. In 
      addition to IL-4, basophils produce IL-6 and tumor necrosis factor (TNF)-alpha in 
      response to immunoglobulin E (IgE) crosslinking. Differentiation of T(H)17 cells 
      requires IL-6 and transforming growth factor (TGF)-beta, but whether basophils play 
      a significant role in T(H)17 induction is unknown. Here we show a role for 
      basophils in T(H)17 cell development by using in vitro T cell differentiation and 
      in vivo T(H)17-mediated inflammation models. Bone marrow derived-basophils (BMBs) 
      and splenic basophils produce significant amounts of IL-6 as well as IL-4 
      following stimulation with IgE crosslink or cholera toxin (CT). In addition, 
      through IL-6 secretion, BMBs cooperate with dendritic cells to promote T(H)17 
      cell differentiation. In the T(H)17 lung inflammation model, basophils are 
      recruited to the inflamed lungs following CT challenge, and T(H)17 responses are 
      significantly reduced in the absence of basophils or IL-6. Furthermore, 
      reconstitution with wild-type, but not IL-6-deficient, basophils restored 
      CT-mediated lung inflammation. Lastly, basophil-deficient mice showed reduced 
      phenotypes of T(H)17-dependent experimental autoimmune encephalomyelitis. 
      Therefore, our results indicate that basophils are an important inducer of T(H)17 
      cell differentiation, which is dependent on IL-6 secretion.
FAU - Yuk, Chae Min
AU  - Yuk CM
AD  - Biomedical Science and Engineering Interdisciplinary Program, Korea Advanced 
      Institute of Science and Technology (KAIST), Daejeon, 34141, Korea.
AD  - Graduate School of Medical Science and Engineering (GSMSE), Biomedical Research 
      Center, KAIST Institute for the Bio-Century, KAIST, Daejeon, 34141, Korea.
FAU - Park, Hyeung Ju
AU  - Park HJ
AD  - Biomedical Science and Engineering Interdisciplinary Program, Korea Advanced 
      Institute of Science and Technology (KAIST), Daejeon, 34141, Korea.
AD  - Graduate School of Medical Science and Engineering (GSMSE), Biomedical Research 
      Center, KAIST Institute for the Bio-Century, KAIST, Daejeon, 34141, Korea.
FAU - Kwon, Bo-In
AU  - Kwon BI
AD  - K-herb Research Center, Korea Institute of Oriental Medicine (KIOM), Daejeon, 
      34054, Korea.
FAU - Lah, Sang Joon
AU  - Lah SJ
AD  - Biomedical Science and Engineering Interdisciplinary Program, Korea Advanced 
      Institute of Science and Technology (KAIST), Daejeon, 34141, Korea.
AD  - Graduate School of Medical Science and Engineering (GSMSE), Biomedical Research 
      Center, KAIST Institute for the Bio-Century, KAIST, Daejeon, 34141, Korea.
FAU - Chang, Jun
AU  - Chang J
AD  - Division of Life and Pharmaceutical Science, Ewha Womans University, Seoul, 
      03760, Korea.
FAU - Kim, Ji-Young
AU  - Kim JY
AD  - Department of Biochemistry and Molecular Biology, Korea University College of 
      Medicine, Seoul, 02841, Korea.
FAU - Lee, Kyung-Mi
AU  - Lee KM
AD  - Department of Biochemistry and Molecular Biology, Korea University College of 
      Medicine, Seoul, 02841, Korea.
FAU - Park, Su-Hyung
AU  - Park SH
AD  - Biomedical Science and Engineering Interdisciplinary Program, Korea Advanced 
      Institute of Science and Technology (KAIST), Daejeon, 34141, Korea.
AD  - Graduate School of Medical Science and Engineering (GSMSE), Biomedical Research 
      Center, KAIST Institute for the Bio-Century, KAIST, Daejeon, 34141, Korea.
FAU - Hong, Seokchan
AU  - Hong S
AD  - Graduate School of Medical Science and Engineering (GSMSE), Biomedical Research 
      Center, KAIST Institute for the Bio-Century, KAIST, Daejeon, 34141, Korea.
AD  - Division of Rheumatology, University of Ulsan College of Medicine, Asan Medical 
      Center, Seoul, 05505, Korea.
FAU - Lee, Seung-Hyo
AU  - Lee SH
AD  - Biomedical Science and Engineering Interdisciplinary Program, Korea Advanced 
      Institute of Science and Technology (KAIST), Daejeon, 34141, Korea.
AD  - Graduate School of Medical Science and Engineering (GSMSE), Biomedical Research 
      Center, KAIST Institute for the Bio-Century, KAIST, Daejeon, 34141, Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170130
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Biomarkers)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-6)
RN  - 9012-63-9 (Cholera Toxin)
SB  - IM
MH  - Animals
MH  - Basophils/*immunology/*metabolism
MH  - Biomarkers
MH  - CD4-Positive T-Lymphocytes/*cytology/*physiology
MH  - Cell Differentiation/*immunology
MH  - Cholera Toxin/adverse effects
MH  - Cytokines/biosynthesis
MH  - Disease Models, Animal
MH  - Immunity
MH  - Immunophenotyping
MH  - Inflammation/etiology/metabolism/pathology
MH  - Interleukin-6/deficiency/*metabolism
MH  - Mice
MH  - Mice, Knockout
MH  - Th17 Cells/*cytology/*physiology
PMC - PMC5278410
COIS- The authors declare no competing financial interests.
EDAT- 2017/01/31 06:00
MHDA- 2018/10/26 06:00
PMCR- 2017/01/30
CRDT- 2017/01/31 06:00
PHST- 2016/09/20 00:00 [received]
PHST- 2016/12/28 00:00 [accepted]
PHST- 2017/01/31 06:00 [entrez]
PHST- 2017/01/31 06:00 [pubmed]
PHST- 2018/10/26 06:00 [medline]
PHST- 2017/01/30 00:00 [pmc-release]
AID - srep41744 [pii]
AID - 10.1038/srep41744 [doi]
PST - epublish
SO  - Sci Rep. 2017 Jan 30;7:41744. doi: 10.1038/srep41744.