PMID- 28134520
OWN - NLM
STAT- MEDLINE
DCOM- 20170526
LR  - 20220408
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 65
IP  - 7
DP  - 2017 Feb 22
TI  - Sea Buckthorn Fruit Oil Extract Alleviates Insulin Resistance through the 
      PI3K/Akt Signaling Pathway in Type 2 Diabetes Mellitus Cells and Rats.
PG  - 1328-1336
LID - 10.1021/acs.jafc.6b04682 [doi]
AB  - Sea buckthorn fruit oil is rich in palmitoleic acid (POA), which has been 
      reported to play roles in many metabolic processes. In this study, a sea 
      buckthorn fruit oil (SBFO) extract was evaluated through in vitro experiments 
      (the doses were 50, 100, 200, and 400 muM) and in vivo experiments (the doses were 
      100, 200, and 300 mg/kg.day) to explore its mechanism of action in the treatment 
      of type 2 diabetes mellitus (T2DM). The results revealed that the SBFO extract 
      effectively increased the glucose uptake from 12.23 +/- 1.09 to 14.90 +/- 1.48 mmol/L 
      in insulin resistance (IR) HepG2 cells, lowered blood glucose (the reductions 
      rates of blood glucose in groups treated with SBFO extract at 200 and 300 
      mg/kg.day were 10.47% and 13.79%, respectively) and improved insulin indices from 
      -6.11 +/- 0.10 to -5.45 +/- 0.31 after 4 weeks treatment with SBFO extract at 300 
      mg/kg.day in T2DM SD rats. RT-PCR and Western blotting analyses suggested that 
      the SBFO extract could promote the expression of phosphatidylinositol-3-kinase 
      (PI3K) and glycogen synthesis (GS) while inhibiting the expression of glycogen 
      synthesis kinase-3beta (GSK-3beta). Thus, the SBFO extract played a positive role in 
      alleviating T2DM through the PI3K/Akt signaling pathway in HepG2 cells, and 
      diabetic rats and could be used for the future development of functional food and 
      dietary supplements.
FAU - Gao, Shan
AU  - Gao S
AD  - Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, 
      Tianjin University of Science and Technology , Tianjin 300457, China.
FAU - Guo, Qing
AU  - Guo Q
AD  - Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, 
      Tianjin University of Science and Technology , Tianjin 300457, China.
FAU - Qin, Chengguang
AU  - Qin C
AD  - Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, 
      Tianjin University of Science and Technology , Tianjin 300457, China.
FAU - Shang, Rui
AU  - Shang R
AD  - Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, 
      Tianjin University of Science and Technology , Tianjin 300457, China.
FAU - Zhang, Zesheng
AU  - Zhang Z
AUID- ORCID: 0000-0002-0173-9355
AD  - Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, 
      Tianjin University of Science and Technology , Tianjin 300457, China.
AD  - Tianjin Food Safety & Low Carbon Manufacturing Collaborative Innovation Center , 
      Tianjin, 300457, China.
LA  - eng
PT  - Journal Article
DEP - 20170210
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Blood Glucose)
RN  - 0 (Insulin)
RN  - 0 (Plant Oils)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - Animals
MH  - Blood Glucose/metabolism
MH  - Diabetes Mellitus, Type 2/*drug therapy/enzymology/metabolism
MH  - Fruit/chemistry
MH  - Hippophae/*chemistry
MH  - Humans
MH  - Insulin/metabolism
MH  - *Insulin Resistance
MH  - Male
MH  - Phosphatidylinositol 3-Kinases/genetics/*metabolism
MH  - Plant Oils/*administration & dosage
MH  - Proto-Oncogene Proteins c-akt/genetics/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction/drug effects
OTO - NOTNLM
OT  - PI3K/Akt signaling pathway
OT  - POA
OT  - SBFO extract
OT  - T2DM
OT  - insulin resistance
EDAT- 2017/01/31 06:00
MHDA- 2017/05/27 06:00
CRDT- 2017/01/31 06:00
PHST- 2017/01/31 06:00 [pubmed]
PHST- 2017/05/27 06:00 [medline]
PHST- 2017/01/31 06:00 [entrez]
AID - 10.1021/acs.jafc.6b04682 [doi]
PST - ppublish
SO  - J Agric Food Chem. 2017 Feb 22;65(7):1328-1336. doi: 10.1021/acs.jafc.6b04682. 
      Epub 2017 Feb 10.