PMID- 28134630
OWN - NLM
STAT- MEDLINE
DCOM- 20180109
LR  - 20181113
IS  - 1473-558X (Electronic)
IS  - 0959-4965 (Print)
IS  - 0959-4965 (Linking)
VI  - 28
IP  - 4
DP  - 2017 Mar 1
TI  - Polyamines preserve connexin 43-mediated gap junctional communication during 
      intracellular hypercalcemia and acidosis.
PG  - 208-213
LID - 10.1097/WNR.0000000000000746 [doi]
AB  - Changes in the regulation, formation, and gating of connexin-based gap junction 
      channels occur in various disorders. It has been shown that H and Ca are involved 
      in the regulation of gap junctional communication. Ischemia-induced intracellular 
      acidification and Ca overload lead to closure of gap junctions and inhibit an 
      exchange by ions and small molecules throughout the network of cells in the 
      heart, brain, and other tissues. In this study, we examined the role of the 
      polyamines in the regulation of connexin 43 (Cx43)-based gap junction channels 
      under elevated intracellular concentrations of hydrogen ([H]i) and calcium 
      ([Ca]i) ions. Experiments, conducted in Novikoff and A172 human glioblastoma 
      cells, which endogenously express Cx43, showed that polyamines prevent 
      downregulation of Cx43-mediated gap junctional communication caused by elevated 
      [Ca]i and [H]i, accompanying ischemic and other pathological conditions. siRNA 
      knockdown of Cx43 significantly reduces gap junctional communication, indicating 
      that Cx43 gap junctions are the targets for spermine regulation.
FAU - Kucheryavykh, Lilia Y
AU  - Kucheryavykh LY
AD  - Departments of aBiochemistry bPhysiology cAnatomy and Cell Biology, School of 
      Medicine, Universidad Central del Caribe, Bayamon, Puerto Rico dDominick P. 
      Purpura Department of Neuroscience, Albert Einstein College of Medicine, New 
      York, New York, USA eInstitute of Cardiology, Lithuanian University of Health 
      Sciences, Kaunas, Lithuania.
FAU - Benedikt, Jan
AU  - Benedikt J
FAU - Cubano, Luis A
AU  - Cubano LA
FAU - Skatchkov, Serguei N
AU  - Skatchkov SN
FAU - Bukauskas, Feliksas F
AU  - Bukauskas FF
FAU - Kucheryavykh, Yuriy V
AU  - Kucheryavykh YV
LA  - eng
GR  - R01 NS072238/NS/NINDS NIH HHS/United States
GR  - G12 MD007583/MD/NIMHD NIH HHS/United States
GR  - SC2 GM095410/GM/NIGMS NIH HHS/United States
GR  - R01 NS065201/NS/NINDS NIH HHS/United States
GR  - SC2 GM102040/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PL  - England
TA  - Neuroreport
JT  - Neuroreport
JID - 9100935
RN  - 0 (Connexin 43)
RN  - 0 (GJA1 protein, human)
RN  - 0 (Polyamines)
RN  - 2FZ7Y3VOQX (Spermine)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Acidosis
MH  - Animals
MH  - Calcium
MH  - Cell Line, Tumor
MH  - Connexin 43/*metabolism
MH  - Gap Junctions/drug effects/*physiology
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Hypercalcemia
MH  - Neurons/metabolism/*physiology
MH  - Polyamines/*administration & dosage
MH  - Rats
MH  - Spermine/administration & dosage
PMC - PMC5298824
MID - NIHMS843150
COIS- Statement of conflicts of interest: none declared
EDAT- 2017/01/31 06:00
MHDA- 2018/01/10 06:00
PMCR- 2018/03/01
CRDT- 2017/01/31 06:00
PHST- 2017/01/31 06:00 [pubmed]
PHST- 2018/01/10 06:00 [medline]
PHST- 2017/01/31 06:00 [entrez]
PHST- 2018/03/01 00:00 [pmc-release]
AID - 10.1097/WNR.0000000000000746 [doi]
PST - ppublish
SO  - Neuroreport. 2017 Mar 1;28(4):208-213. doi: 10.1097/WNR.0000000000000746.