PMID- 28135025
OWN - NLM
STAT- MEDLINE
DCOM- 20180305
LR  - 20180305
IS  - 1442-200X (Electronic)
IS  - 1328-8067 (Linking)
VI  - 59
IP  - 5
DP  - 2017 May
TI  - Acute liver dysfunction not resulting from hepatitis virus in immunocompetent 
      children.
PG  - 551-556
LID - 10.1111/ped.13249 [doi]
AB  - BACKGROUND: The aim of the present study was to clarify the roles of 
      cytomegalovirus (CMV), Epstein-Barr virus (EBV), and human herpesvirus 6 (HHV-6) 
      in immunocompetent children with acute liver dysfunction not resulting from 
      hepatitis virus. METHODS: Sixty-eight children (median age, 3 years) hospitalized 
      as a result of acute liver dysfunction were enrolled in this study. Hepatitis A, 
      B, and C were excluded. The prevalence of CMV, EBV, and HHV-6 and viral DNA load 
      in whole blood was prospectively evaluated on multiplex real-time polymerase 
      chain reaction (PCR). RESULTS: Of the 68 children with acute liver dysfunction, 
      multiplex real-time PCR was positive in 30 (44%). CMV, EBV, and HHV-6 DNA were 
      detected in 13 (19%), 14 (21%), and seven (10%), respectively. Serum CMV 
      immunoglobulin (Ig)G/IgM and EBV viral capsid antigen IgG/IgM were measured in 40 
      (CMV DNA positive, n = 10; negative, n = 30) and 45 (EBV DNA positive, n = 14; 
      negative, n = 31) of the 68 children, respectively. Eighteen percent (CMV, 7/40) 
      and 9% (EBV, 4/45) were positive for both PCR and viral-specific IgM. There was 
      no significant difference in CMV and EBV viral load between IgM-positive and 
      -negative children with viremia. CONCLUSIONS: CMV, EBV, and HHV-6 DNA were 
      frequently detected in immunocompetent children with acute liver dysfunction, but 
      primary CMV and EBV infection were confirmed in 10-20% of the children with acute 
      liver dysfunction. The combination of PCR assay and serology is necessary to make 
      a diagnosis of acute liver dysfunction due to primary CMV, EBV and/or HHV-6 
      infection in immunocompetent children.
CI  - (c) 2017 Japan Pediatric Society.
FAU - Tsunoda, Tomoyuki
AU  - Tsunoda T
AD  - Department of Pediatric Hepatology and Gastroenterology, Eastern Yokohama 
      Hospital, Kanagawa, Japan.
FAU - Inui, Ayano
AU  - Inui A
AD  - Department of Pediatric Hepatology and Gastroenterology, Eastern Yokohama 
      Hospital, Kanagawa, Japan.
FAU - Iwasawa, Kentaro
AU  - Iwasawa K
AD  - Department of Pediatric Hepatology and Gastroenterology, Eastern Yokohama 
      Hospital, Kanagawa, Japan.
FAU - Oikawa, Manari
AU  - Oikawa M
AD  - Department of Pediatric Hepatology and Gastroenterology, Eastern Yokohama 
      Hospital, Kanagawa, Japan.
FAU - Sogo, Tsuyoshi
AU  - Sogo T
AD  - Department of Pediatric Hepatology and Gastroenterology, Eastern Yokohama 
      Hospital, Kanagawa, Japan.
FAU - Komatsu, Haruki
AU  - Komatsu H
AD  - Department of Pediatrics, Sakura Medical Center, Toho University, Chiba, Japan.
FAU - Ito, Yoshinori
AU  - Ito Y
AD  - Department of Pediatrics, Nagoya University Graduate School of Medicine, Aichi, 
      Japan.
FAU - Fujisawa, Tomoo
AU  - Fujisawa T
AD  - Department of Pediatric Hepatology and Gastroenterology, Eastern Yokohama 
      Hospital, Kanagawa, Japan.
LA  - eng
SI  - GENBANK/AJ507799
SI  - GENBANK/NC001347
SI  - GENBANK/AF157706
PT  - Journal Article
DEP - 20170321
PL  - Australia
TA  - Pediatr Int
JT  - Pediatrics international : official journal of the Japan Pediatric Society
JID - 100886002
SB  - IM
CIN - Pediatr Int. 2017 Aug;59(8):947. PMID: 28804977
MH  - Acute Disease
MH  - Adolescent
MH  - Child
MH  - Child, Preschool
MH  - Cytomegalovirus Infections/*complications/diagnosis/epidemiology/immunology
MH  - Epstein-Barr Virus Infections/*complications/diagnosis/epidemiology/immunology
MH  - Female
MH  - Hepatic Insufficiency/immunology/*virology
MH  - Herpesvirus 6, Human/*isolation & purification
MH  - Humans
MH  - *Immunocompetence
MH  - Infant
MH  - Infant, Newborn
MH  - Male
MH  - Multiplex Polymerase Chain Reaction
MH  - Prevalence
MH  - Prospective Studies
MH  - Real-Time Polymerase Chain Reaction
MH  - Roseolovirus Infections/*complications/diagnosis/epidemiology/immunology
MH  - Viral Load
OTO - NOTNLM
OT  - herpesvirus
OT  - immunocompetent
OT  - liver failure
OT  - multiplex
OT  - real-time polymerase chain reaction
EDAT- 2017/01/31 06:00
MHDA- 2018/03/06 06:00
CRDT- 2017/01/31 06:00
PHST- 2016/03/14 00:00 [received]
PHST- 2016/11/23 00:00 [revised]
PHST- 2016/12/26 00:00 [accepted]
PHST- 2017/01/31 06:00 [pubmed]
PHST- 2018/03/06 06:00 [medline]
PHST- 2017/01/31 06:00 [entrez]
AID - 10.1111/ped.13249 [doi]
PST - ppublish
SO  - Pediatr Int. 2017 May;59(5):551-556. doi: 10.1111/ped.13249. Epub 2017 Mar 21.