PMID- 28135314
OWN - NLM
STAT- MEDLINE
DCOM- 20170809
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 1
DP  - 2017
TI  - The Evolution of Vicia ramuliflora (Fabaceae) at Tetraploid and Diploid Levels 
      Revealed with FISH and RAPD.
PG  - e0170695
LID - 10.1371/journal.pone.0170695 [doi]
LID - e0170695
AB  - Vicia ramuliflora L. is a widely distributed species in Eurasia with high 
      economic value. For past 200 years, it has evolved a tetraploid cytotype and new 
      subspecies at the diploid level. Based on taxonomy, cytogeography and other lines 
      of evidence, previous studies have provided valuable information about the 
      evolution of V. ramuliflora ploidy level, but due to the limited resolution of 
      traditional methods, important questions remain. In this study, fluorescence in 
      situ hybridization (FISH) and random amplified polymorphic DNA (RAPD) were used 
      to analyze the evolution of V. ramuliflora at the diploid and tetraploid levels. 
      Our aim was to reveal the genomic constitution and parents of the tetraploid V. 
      ramuliflora and the relationships among diploid V. ramuliflora populations. Our 
      study showed that the tetraploid cytotype of V. ramuliflora at Changbai Mountains 
      (M) has identical 18S and 5S rDNA distribution patterns with the diploid 
      Hengdaohezi population (B) and the diploid Dailing population (H). However, UPGMA 
      clustering, Neighbor-Joining clustering and principal coordinates analysis based 
      on RAPD showed that the tetraploid cytotype (M) has more close relationships with 
      Qianshan diploid population T. Based on our results and the fact that 
      interspecific hybridization among Vicia species is very difficult, we think that 
      the tetraploid V. ramuliflora is an autotetraploid and its genomic origin still 
      needs further study. In addition, our study also found that Qianshan diploid 
      population (T) had evolved distinct new traits compared with other diploid 
      populations, which hints that V. ramuliflora evolved further at diploid level. We 
      suggest that diploid population T be re-classified as a new subspecies.
FAU - Han, Ying
AU  - Han Y
AD  - School of Life Science and Engineering, Southwest University of Science and 
      Technology, Mianyang, China.
FAU - Liu, Yuan
AU  - Liu Y
AD  - College of Life Sciences, Tianjin Normal University, Tianjin, China.
FAU - Wang, Haoyou
AU  - Wang H
AD  - School of life science and technology, Harbin Normal University, Harbin, China.
FAU - Liu, Xiangjun
AU  - Liu X
AD  - College of Life Sciences, Tianjin Normal University, Tianjin, China.
LA  - eng
PT  - Journal Article
DEP - 20170130
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (DNA Probes)
RN  - 0 (Genetic Markers)
RN  - 0 (RNA, Ribosomal, 5S)
SB  - IM
MH  - *Biological Evolution
MH  - China
MH  - Chromosomes, Plant/genetics
MH  - DNA Probes/metabolism
MH  - *Diploidy
MH  - Genetic Markers
MH  - Geography
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Metaphase
MH  - Phylogeny
MH  - Polymorphism, Genetic
MH  - RNA, Ribosomal, 5S/genetics
MH  - Random Amplified Polymorphic DNA Technique/*methods
MH  - *Tetraploidy
MH  - Vicia/*genetics
PMC - PMC5279728
COIS- The authors have declared that no competing interests exist.
EDAT- 2017/01/31 06:00
MHDA- 2017/08/10 06:00
PMCR- 2017/01/30
CRDT- 2017/01/31 06:00
PHST- 2016/06/16 00:00 [received]
PHST- 2017/01/09 00:00 [accepted]
PHST- 2017/01/31 06:00 [entrez]
PHST- 2017/01/31 06:00 [pubmed]
PHST- 2017/08/10 06:00 [medline]
PHST- 2017/01/30 00:00 [pmc-release]
AID - PONE-D-16-24272 [pii]
AID - 10.1371/journal.pone.0170695 [doi]
PST - epublish
SO  - PLoS One. 2017 Jan 30;12(1):e0170695. doi: 10.1371/journal.pone.0170695. 
      eCollection 2017.