PMID- 28138695
OWN - NLM
STAT- MEDLINE
DCOM- 20170713
LR  - 20170713
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Linking)
VI  - 15
IP  - 3
DP  - 2017 Mar
TI  - Involvement of retinoblastoma-associated protein 48 during photodynamic therapy 
      of cervical cancer cells.
PG  - 1393-1400
LID - 10.3892/mmr.2017.6151 [doi]
AB  - 5-Aminolevulinic acid-mediated photodynamic therapy (ALA‑PDT) is an effective 
      treatment option for cervical intraepithelial neoplasia, the precancerous lesion 
      of cervical cancer, and early cervical cancer, particularly for young or 
      nulliparous women who want to remain fertile. A previous report described the 
      involvement of histone deacetylases (HDAC) during ALA‑PDT mediated apoptosis in 
      the cerebral cortex of a mouse model. Retinoblastoma‑associated protein 48 
      (RbAp48), a highly abundant component of HDACs, is a critical mediator that 
      controls the transforming activity of human papillomavirus 16 in cervical cancer 
      cells. The aim of the present study was to investigate the involvement of RbAp48 
      in ALA‑PDT‑induced cell death in cervical cancer cells. RbAp48 was significantly 
      upregulated in cervical cancer cell lines treated with ALA‑PDT, including SiHa 
      and HeLa cells. To establish the relevance of RbAp48 and the efficacy of ALA‑PDT 
      in cervical cancer cells, the effect of ALA‑PDT was investigated in SiHa or HeLa 
      cells following the depletion of RbAp48 by small interfering RNA (siRNA). 
      Reduction of RbAp48 led to the reduced suppression of proliferation and apoptosis 
      induced by ALA‑PDT in cervical cancer cells, which was associated with a 
      reduction in tumor suppressor protein 53 (p53), retinoblastoma (Rb), 
      apoptosis‑related enzyme caspase‑3, and increased levels of the oncogenic genes, 
      human papillomavirus E6 and E7. These results provide evidence that RbAp48 is an 
      important contributor to the efficacy of ALA‑PDT in cervical cancer cells. RbAp48 
      may be a therapeutic target that may help to improve the treatment of cervical 
      cancer.
FAU - Wu, Shuxia
AU  - Wu S
AD  - Department of Gynecology and Obstetrics, Qilu Hospital of Shandong University, 
      Jinan, Shandong 250012, P.R. China.
FAU - Wang, Lijun
AU  - Wang L
AD  - Department of Pediatrics, Shandong Provincial Hospital Affiliated to Shandong 
      University, Jinan, Shandong 250021, P.R. China.
FAU - Ren, Xingye
AU  - Ren X
AD  - Department of Gynecology and Obstetrics, The Fifth People's Hospital of Jinan, 
      Jinan, Shandong 250021, P.R. China.
FAU - Pan, Yulu
AU  - Pan Y
AD  - Department of Gynecology and Obstetrics, The Fifth People's Hospital of Jinan, 
      Jinan, Shandong 250021, P.R. China.
FAU - Peng, Yan
AU  - Peng Y
AD  - Department of Gynecology and Obstetrics, The Fifth People's Hospital of Jinan, 
      Jinan, Shandong 250021, P.R. China.
FAU - Zou, Xiaoyan
AU  - Zou X
AD  - Department of Gynecology and Obstetrics, The Fifth People's Hospital of Jinan, 
      Jinan, Shandong 250021, P.R. China.
FAU - Shi, Cuige
AU  - Shi C
AD  - National Research Institute of Family Planning, Beijing 100081, P.R. China.
FAU - Zhang, Youzhong
AU  - Zhang Y
AD  - Department of Gynecology and Obstetrics, Qilu Hospital of Shandong University, 
      Jinan, Shandong 250012, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20170126
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Photosensitizing Agents)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Retinoblastoma-Binding Protein 4)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects/genetics/radiation effects
MH  - Cell Line, Tumor
MH  - Female
MH  - Gene Expression Regulation, Neoplastic/drug effects/radiation effects
MH  - Gene Silencing
MH  - HeLa Cells
MH  - Humans
MH  - *Light
MH  - Mice
MH  - *Photochemotherapy
MH  - Photosensitizing Agents/*pharmacology
MH  - RNA Interference
MH  - RNA, Messenger/genetics/metabolism
MH  - RNA, Small Interfering
MH  - Retinoblastoma-Binding Protein 4/*genetics/metabolism
EDAT- 2017/02/01 06:00
MHDA- 2017/07/14 06:00
CRDT- 2017/02/01 06:00
PHST- 2015/11/14 00:00 [received]
PHST- 2016/11/24 00:00 [accepted]
PHST- 2017/02/01 06:00 [pubmed]
PHST- 2017/07/14 06:00 [medline]
PHST- 2017/02/01 06:00 [entrez]
AID - 10.3892/mmr.2017.6151 [doi]
PST - ppublish
SO  - Mol Med Rep. 2017 Mar;15(3):1393-1400. doi: 10.3892/mmr.2017.6151. Epub 2017 Jan 
      26.