PMID- 28138774
OWN - NLM
STAT- MEDLINE
DCOM- 20170428
LR  - 20181202
IS  - 1432-8798 (Electronic)
IS  - 0304-8608 (Linking)
VI  - 162
IP  - 5
DP  - 2017 May
TI  - Effect of porcine circovirus type 2 (PCV2) on the function of splenic CD11c(+) 
      dendritic cells in mice.
PG  - 1289-1298
LID - 10.1007/s00705-017-3221-8 [doi]
AB  - Porcine circovirus-associated disease (PCVAD) caused by porcine circovirus type 2 
      (PCV2) is an important disease in the global pig industry. Dendritic cells (DCs) 
      are the primary immune cells capable of initiating adaptive immune responses as 
      well as major target cells of PCV2. To determine whether PCV2 affects the immune 
      functions of DCs, we evaluated the expression of endocytosis and co-stimulatory 
      molecules on DCs (CD11c(+)) from PCV2-infected mouse spleen by flow cytometry 
      (FCM). We also analyzed the main cytokines secreted by DCs (CD11c(+)) and 
      activation of CD4(+) and CD8(+) T cells by DCs (CD11c(+)) through measurement of 
      cytokine secretion, using ELISA. Compared with control mice, PCV2 did not affect 
      the endocytic activity of DCs but it significantly enhanced TNF-alpha secretion and 
      markedly decreased IFN-alpha secretion. Subsets of CD40(+), MHCII(+) CD40(+) and 
      CD137L(+) CD86(+) DCs did not increase obviously, but MHCII(+) CD40(-) and 
      CD137L(-) CD80(+)/CD86(+) DCs increased significantly in PCV2-infected mouse 
      spleen. Under the stimulation of DCs from PCV2-infected mouse, secretion of IFN-gamma 
      by CD4(+) and CD8(+) T cells and of IL-12 by CD8(+) T cells was significantly 
      lower than in control mice, while secretion of IL-4 by CD4(+) T cells was 
      remarkably higher. These results indicate that PCV2 modulates cytokine secretion 
      and co-stimulatory molecule expression of DCs, and alters activation of CD4(+) 
      and CD8(+) T cells by DCs. The immunomodulatory effects of PCV2 on DCs might be 
      related to the host's immune dysfunction and persistent infection with this 
      virus.
FAU - Wang, Xiaobo
AU  - Wang X
AD  - College of Veterinary Medicine, Hebei Agricultural University, No. 2596 Lekai 
      Southern Street, Baoding, 071000, Hebei, China.
FAU - Chen, Ligong
AU  - Chen L
AD  - College of Veterinary Medicine, Hebei Agricultural University, No. 2596 Lekai 
      Southern Street, Baoding, 071000, Hebei, China.
FAU - Yuan, Wanzhe
AU  - Yuan W
AD  - College of Veterinary Medicine, Hebei Agricultural University, No. 2596 Lekai 
      Southern Street, Baoding, 071000, Hebei, China.
FAU - Li, Yanqin
AU  - Li Y
AD  - College of Veterinary Medicine, Hebei Agricultural University, No. 2596 Lekai 
      Southern Street, Baoding, 071000, Hebei, China.
FAU - Li, Limin
AU  - Li L
AD  - College of Veterinary Medicine, Hebei Agricultural University, No. 2596 Lekai 
      Southern Street, Baoding, 071000, Hebei, China.
FAU - Li, Tanqing
AU  - Li T
AD  - College of Veterinary Medicine, Hebei Agricultural University, No. 2596 Lekai 
      Southern Street, Baoding, 071000, Hebei, China.
FAU - Li, Huanrong
AU  - Li H
AD  - College of Animal Science and Technology, Beijing University of Agriculture, No. 
      7 Beinong Road, Changping District, Beijing, 102206, China. lihuanrong1@126.com.
FAU - Song, Qinye
AU  - Song Q
AD  - College of Veterinary Medicine, Hebei Agricultural University, No. 2596 Lekai 
      Southern Street, Baoding, 071000, Hebei, China. songqinye@126.com.
LA  - eng
PT  - Journal Article
DEP - 20170130
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (CD11c Antigen)
RN  - 0 (Il12a protein, mouse)
RN  - 0 (Interferon-alpha)
RN  - 0 (Interleukin-12 Subunit p35)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 207137-56-2 (Interleukin-4)
SB  - IM
MH  - Animals
MH  - CD11c Antigen/*immunology
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - CD8-Positive T-Lymphocytes/immunology
MH  - Circovirus/*immunology
MH  - Dendritic Cells/*immunology
MH  - Endocytosis/*immunology
MH  - Female
MH  - Interferon-alpha/metabolism
MH  - Interleukin-12 Subunit p35/metabolism
MH  - Interleukin-4/metabolism
MH  - Lymphocyte Activation/immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Spleen/*cytology/immunology
MH  - Swine/virology
MH  - Swine Diseases/virology
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2017/02/01 06:00
MHDA- 2017/04/30 06:00
CRDT- 2017/02/01 06:00
PHST- 2016/08/03 00:00 [received]
PHST- 2017/01/09 00:00 [accepted]
PHST- 2017/02/01 06:00 [pubmed]
PHST- 2017/04/30 06:00 [medline]
PHST- 2017/02/01 06:00 [entrez]
AID - 10.1007/s00705-017-3221-8 [pii]
AID - 10.1007/s00705-017-3221-8 [doi]
PST - ppublish
SO  - Arch Virol. 2017 May;162(5):1289-1298. doi: 10.1007/s00705-017-3221-8. Epub 2017 
      Jan 30.