PMID- 28138935
OWN - NLM
STAT- MEDLINE
DCOM- 20170515
LR  - 20240109
IS  - 1436-2236 (Electronic)
IS  - 1436-2228 (Linking)
VI  - 19
IP  - 1
DP  - 2017 Feb
TI  - Immunostimulatory Effect of Enzyme-Modified Hizikia fusiformein a Mouse Model In 
      Vitro and Ex Vivo.
PG  - 65-75
LID - 10.1007/s10126-017-9727-y [doi]
AB  - Hizikia fusiforme, a brown seaweed, has been utilized as a health food and in 
      traditional medicine. In this study, we investigated whether enzyme-modified H. 
      fusiforme extracts (EH) have immunological effects compared with normal H. 
      fusiforme extracts (NH). The effects of NH and EH on immune responses were 
      investigated by assessing nitric oxide (NO) production, phagocytosis, and 
      cytokine secretion in RAW 264.7 murine macrophages and mice. Also, fucosterol was 
      evaluated to find the active component of NH and EH by addressing cytotoxicity 
      test and NO production. Both of NH and EH significantly increased cell viability 
      and NO synthesis. Tumor necrosis factor-alpha (TNF-alpha) expression was more induced by 
      EH with LPS treatment. Phagocytic activity, as the primary function of 
      macrophages, was markedly induced by EH treatment. Additionally, EH encouraged 
      splenocyte proliferation and recovered the levels of cytokines IL-1beta, IL-6, and 
      TNF-alpha in mice. Finally, fucosterol increased NO production with no cytotoxicity, 
      which means that fucosterol is an active component of EH. In conclusion, EH has 
      the potential to modulate immune function and could offer positive therapeutic 
      effect for immune system diseases.
FAU - Park, Sang-Yong
AU  - Park SY
AD  - Department of Oriental Medicinal Materials & Processing, College of Life Science, 
      Kyung Hee University, Yongin-si, South Korea.
FAU - Hwang, Eunson
AU  - Hwang E
AD  - Department of Oriental Medicinal Materials & Processing, College of Life Science, 
      Kyung Hee University, Yongin-si, South Korea.
FAU - Shin, Yu-Kyong
AU  - Shin YK
AD  - Department of Oriental Medicinal Materials & Processing, College of Life Science, 
      Kyung Hee University, Yongin-si, South Korea.
FAU - Lee, Don-Gil
AU  - Lee DG
AD  - Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, 
      Yongin-Si, South Korea.
FAU - Yang, Jung-Eun
AU  - Yang JE
AD  - Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, 
      Yongin-Si, South Korea.
FAU - Park, Jae-Hee
AU  - Park JH
AD  - Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, 
      Yongin-Si, South Korea.
FAU - Yi, Tae-Hoo
AU  - Yi TH
AD  - Department of Oriental Medicinal Materials & Processing, College of Life Science, 
      Kyung Hee University, Yongin-si, South Korea. drhoo@khu.ac.kr.
AD  - Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, 
      Yongin-Si, South Korea. drhoo@khu.ac.kr.
LA  - eng
PT  - Journal Article
DEP - 20170130
PL  - United States
TA  - Mar Biotechnol (NY)
JT  - Marine biotechnology (New York, N.Y.)
JID - 100892712
RN  - 0 (IL1B protein, mouse)
RN  - 0 (Immunologic Factors)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-6)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (interleukin-6, mouse)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 504ZAM710C (fucosterol)
RN  - 99WUK5D0Y8 (Stigmasterol)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - Gene Expression
MH  - Immunologic Factors/isolation & purification/*pharmacology
MH  - Interleukin-1beta/genetics/immunology
MH  - Interleukin-6/genetics/immunology
MH  - Lipopolysaccharides/antagonists & inhibitors/pharmacology
MH  - Lymphocytes/cytology/*drug effects/immunology
MH  - Macrophages/cytology/drug effects/immunology
MH  - Mice
MH  - Nitric Oxide/agonists/biosynthesis
MH  - Phaeophyceae/*chemistry
MH  - Phagocytosis/*drug effects
MH  - Primary Cell Culture
MH  - Seaweed/*chemistry
MH  - Stigmasterol/*analogs & derivatives/isolation & purification/pharmacology
MH  - Tumor Necrosis Factor-alpha/genetics/immunology
OTO - NOTNLM
OT  - Enzyme-modified
OT  - Ex vivo
OT  - Fucosterol
OT  - Hizikia fusiforme
OT  - Immunostimulatory effect
EDAT- 2017/02/01 06:00
MHDA- 2017/05/16 06:00
CRDT- 2017/02/01 06:00
PHST- 2016/03/31 00:00 [received]
PHST- 2017/01/09 00:00 [accepted]
PHST- 2017/02/01 06:00 [pubmed]
PHST- 2017/05/16 06:00 [medline]
PHST- 2017/02/01 06:00 [entrez]
AID - 10.1007/s10126-017-9727-y [pii]
AID - 10.1007/s10126-017-9727-y [doi]
PST - ppublish
SO  - Mar Biotechnol (NY). 2017 Feb;19(1):65-75. doi: 10.1007/s10126-017-9727-y. Epub 
      2017 Jan 30.