PMID- 28145578
OWN - NLM
STAT- MEDLINE
DCOM- 20171102
LR  - 20181202
IS  - 1097-4644 (Electronic)
IS  - 0730-2312 (Linking)
VI  - 118
IP  - 8
DP  - 2017 Aug
TI  - Comparision of Gingival and Umbilical Cord Stem Cells Based on Its Modulus and 
      Neuronal Differentiation.
PG  - 2000-2008
LID - 10.1002/jcb.25918 [doi]
AB  - The availability of Human Umbilical Cord-derived Mesenchymal Stem Cells (HUCMSCs) 
      from a single sex being a major limitation for the utilization of a potential 
      stem cell, it is highly desirable to utilize, an autogenous pluripotent cell with 
      desirable biological and mechanical properties in clinical situations. Comparison 
      of Human Gingival Mesenchymal Stem Cells (HGMSCs) with HUCMSCs demonstrates; MSCs 
      derived from gingiva have higher proliferation rate and higher population 
      doubling time than Umbilical Cord. Unlike HUCMSCs, immunofluorescence studies 
      showed the presence of pluripotency markers OCT-4 and NANOG predominantly in the 
      cytoplasm of HGMSCs which was confirmed by Western blot. The mechanical property, 
      such as modulus of elasticity of HGMSCs, is on par with HUCMSCs, but the surface 
      roughness found to be lesser in HGMSCs, which may suggest HGMSCs greater adhesive 
      property to the extracellular matrix. There is a marginal difference in the 
      neuronal differentiation rate between HGMSCs and HUCMSCs; both the cells 
      expressed positivity for several neuronal lineage markers. Hence, HGMSCs 
      represent an autogenous source of mesenchymal stem cells, which are easy to 
      procure with least morbidity, multipotent in nature with desirable biological, 
      and mechanical properties, probably an ideal candidate for clinical applications. 
      J. Cell. Biochem. 118: 2000-2008, 2017. (c) 2017 Wiley Periodicals, Inc.
CI  - (c) 2017 Wiley Periodicals, Inc.
FAU - Subbarayan, Rajasekaran
AU  - Subbarayan R
AD  - Center for Regenerative Medicine Stem Cell Research, Central Research Facility, 
      Sri Ramachandra University, Chennai, India.
FAU - Murugan Girija, Dinesh
AU  - Murugan Girija D
AD  - Center for Indian Systems of Medicine Quality Assurance and Standardization, 
      Central Research Facility, Sri Ramachandra University, Chennai, India.
FAU - Mukherjee, Joynarayan
AU  - Mukherjee J
AD  - Nano Functional Materials Technology Center, Materials Science Research Center 
      and Department of Physics, Indian Institute of Technology Madras, Chennai, India.
FAU - Mamidanna, Sri Ramachandra Rao
AU  - Mamidanna SRR
AD  - Nano Functional Materials Technology Center, Materials Science Research Center 
      and Department of Physics, Indian Institute of Technology Madras, Chennai, India.
FAU - Ranga Rao, Suresh
AU  - Ranga Rao S
AD  - Faculty of Dental Sciences and Center for Regenerative Medicine and Stem Cell 
      Research, Department of Periodontology and Implantology, Sri Ramachandra 
      University, Chennai, India.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20170425
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (Antigens, Nuclear)
RN  - 0 (Biomarkers)
RN  - 0 (MAP2 protein, human)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (NANOG protein, human)
RN  - 0 (Nanog Homeobox Protein)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Octamer Transcription Factor-3)
RN  - 0 (POU5F1 protein, human)
RN  - 0 (TUBB3 protein, human)
RN  - 0 (Tubulin)
RN  - 0 (neuronal nuclear antigen NeuN, human)
SB  - IM
MH  - Antigens, Nuclear/*genetics/metabolism
MH  - Biomarkers/metabolism
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - Elastic Modulus
MH  - Fetal Blood/*cytology/metabolism
MH  - Gene Expression
MH  - Gingiva/*cytology/metabolism
MH  - Humans
MH  - Mesenchymal Stem Cells/*cytology/metabolism
MH  - Microscopy, Atomic Force
MH  - Microtubule-Associated Proteins/genetics/metabolism
MH  - Nanog Homeobox Protein/genetics/metabolism
MH  - Nerve Tissue Proteins/*genetics/metabolism
MH  - Neurons/*cytology/metabolism
MH  - Octamer Transcription Factor-3/genetics/metabolism
MH  - Primary Cell Culture
MH  - Tubulin/genetics/metabolism
OTO - NOTNLM
OT  - HUMAN GINGIVA
OT  - MESENCHYMAL STEM CELL
OT  - MODULUS
OT  - NEURONAL DIFFERENTIATION
OT  - UMBILICAL CORD
EDAT- 2017/02/02 06:00
MHDA- 2017/11/03 06:00
CRDT- 2017/02/02 06:00
PHST- 2017/01/27 00:00 [received]
PHST- 2017/01/30 00:00 [accepted]
PHST- 2017/02/02 06:00 [pubmed]
PHST- 2017/11/03 06:00 [medline]
PHST- 2017/02/02 06:00 [entrez]
AID - 10.1002/jcb.25918 [doi]
PST - ppublish
SO  - J Cell Biochem. 2017 Aug;118(8):2000-2008. doi: 10.1002/jcb.25918. Epub 2017 Apr 
      25.