PMID- 28146074 OWN - NLM STAT- MEDLINE DCOM- 20170420 LR - 20181113 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 18 IP - 2 DP - 2017 Jan 29 TI - Lannea coromandelica (Houtt.) Merr. Induces Heme Oxygenase 1 (HO-1) Expression and Reduces Oxidative Stress via the p38/c-Jun N-Terminal Kinase-Nuclear Factor Erythroid 2-Related Factor 2 (p38/JNK-NRF2)-Mediated Antioxidant Pathway. LID - 10.3390/ijms18020266 [doi] LID - 266 AB - The leaves of Lannea coromandelica (Houtt.) Merr. are used in the Garo, Pahan, and Teli tribal communities of Bangladesh as a traditional medicinal plant to treat hepatitis, diabetes, ulcers, heart disease, and dysentery. However, there have been limited phytochemical and biological studies on the bark of L. coromandelica. This study aimed to investigate the antioxidant activities of L. coromandelica bark extract (LCBE) and the underlying mechanism using RAW 264.7 cells. The LCBE was analysed by high-pressure liquid chromatography (HPLC) to detect its key polyphenolic compounds. Various in vitro antioxidant assays were performed using RAW 264.7 cells to assess the antioxidant effects of the LCBE and to understand the underlying molecular mechanism. HPLC revealed the presence of gallic acid, (-)-epigallocatechin-3-gallate, catechin, chlorogenic acid, and caffeic acid in the LCBE. The extract showed a very potent capacity to scavenge numerous free radicals through hydrogen atom transfer and/or electron donation and also quenched cellular reactive oxygen species (ROS) generation without showing any toxicity. The LCBE was found to combat the oxidative stress by enhancing the expression, at both transcriptional and translational levels, of primary antioxidant enzymes as well as phase II detoxifying enzymes, especially heme oxygenase 1, through the upregulation of the nuclear factor erythroid 2-related factor 2 (NRF2)-mediated pathway in RAW 264.7 cells via the phosphorylation of p38 kinase and c-Jun N-terminal kinase (JNK). The LCBE exhibited strong antioxidant activities and mitigated the cellular ROS production. These results provide scientific evidence of its potential as an ideal applicant for a cost-effective, readily available, and natural phytochemical, as well as a strategy for preventing diseases associated with oxidative stress and attenuating disease progress. FAU - Alam, Md Badrul AU - Alam MB AD - Department of Food Science and Biotechnology, Graduate School, Kyungpook National University, Daegu 41566, Korea. mbalam@knu.ac.kr. FAU - Kwon, Kyoo-Ri AU - Kwon KR AD - Department of Food Science and Biotechnology, Graduate School, Kyungpook National University, Daegu 41566, Korea. rbfl0116@daum.net. FAU - Lee, Seok-Hyun AU - Lee SH AD - Department of Food Science and Biotechnology, Graduate School, Kyungpook National University, Daegu 41566, Korea. sam4408@naver.com. FAU - Lee, Sang-Han AU - Lee SH AD - Department of Food Science and Biotechnology, Graduate School, Kyungpook National University, Daegu 41566, Korea. sang@knu.ac.kr. AD - Food and Bio-Industry Research Institute, Kyungpook National University, Daegu 41566, Korea. sang@knu.ac.kr. LA - eng PT - Journal Article DEP - 20170129 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Antioxidants) RN - 0 (NF-E2-Related Factor 2) RN - 0 (Plant Extracts) RN - 0 (Reactive Oxygen Species) RN - EC 1.14.14.18 (Heme Oxygenase-1) RN - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases) RN - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases) SB - IM MH - Anacardiaceae/*chemistry MH - Animals MH - Antioxidants/chemistry/pharmacology MH - Cell Line MH - Cell Nucleus MH - Chromatography, High Pressure Liquid MH - Gene Expression Regulation, Enzymologic/genetics MH - Heme Oxygenase-1/*metabolism MH - JNK Mitogen-Activated Protein Kinases/*metabolism MH - Mice MH - NF-E2-Related Factor 2/*metabolism MH - Oxidative Stress/*drug effects/genetics MH - Phosphorylation MH - Plant Bark/chemistry MH - Plant Extracts/chemistry/*pharmacology MH - Protein Transport MH - Reactive Oxygen Species/metabolism MH - Signal Transduction/*drug effects MH - p38 Mitogen-Activated Protein Kinases/*metabolism PMC - PMC5343802 OTO - NOTNLM OT - Lannea coromandelica OT - antioxidant OT - heme oxygenase 1 (HO-1) OT - nuclear factor erythroid 2-related factor 2 (NRF2) COIS- The authors declare no conflict of interest. EDAT- 2017/02/02 06:00 MHDA- 2017/04/21 06:00 PMCR- 2017/02/01 CRDT- 2017/02/02 06:00 PHST- 2016/12/21 00:00 [received] PHST- 2017/01/22 00:00 [revised] PHST- 2017/01/23 00:00 [accepted] PHST- 2017/02/02 06:00 [entrez] PHST- 2017/02/02 06:00 [pubmed] PHST- 2017/04/21 06:00 [medline] PHST- 2017/02/01 00:00 [pmc-release] AID - ijms18020266 [pii] AID - ijms-18-00266 [pii] AID - 10.3390/ijms18020266 [doi] PST - epublish SO - Int J Mol Sci. 2017 Jan 29;18(2):266. doi: 10.3390/ijms18020266.