PMID- 28147306
OWN - NLM
STAT- MEDLINE
DCOM- 20170608
LR  - 20220408
IS  - 1873-264X (Electronic)
IS  - 0731-7085 (Linking)
VI  - 137
DP  - 2017 Apr 15
TI  - Rapid determination of 30 bioactive constituents in XueBiJing injection using 
      ultra high performance liquid chromatography-high resolution hybrid 
      quadrupole-orbitrap mass spectrometry coupled with principal component analysis.
PG  - 220-228
LID - S0731-7085(16)30724-5 [pii]
LID - 10.1016/j.jpba.2017.01.024 [doi]
AB  - Xuebijing injection (XBJ) is a traditional Chinese herbal prescription widely 
      used in the treatment of sepsis. Extensive chemical studies revealed that XBJ 
      injection contains amino acids, phenolic acids, flavonoid glycosides, 
      terpeneglycosides and phthalides. In this study, the applicability of ultra high 
      performance liquid chromatography coupled with high resolution hybrid 
      quadruple-orbitrap mass spectrometry (UHPLC-Q-Orbitrap MS) for the simultaneous 
      quantitative analysis of 30 bioactive constituents in XueBiJing injection (XBJ) 
      was investigated. The mass spectrometer was operated in full MS scan mode. The 
      use of 70,000FWHM mass resolution and narrow mass windows (5ppm) could 
      effectively improve the selectivity of the method. Separation was achieved on a 
      Waters ACQUITY UPLC((R)) HSS C(18) column (2.1mmx100mm, 1.8mum) with a gradient 
      mobile phase consisting of acetonitrile-water (containing 10mM ammonium acetate) 
      at a flow rate of 0.2mL/min. Satisfactory linearity was achieved within wide 
      linear range and all correlation coefficients (r) of analytes were more than 
      0.9996. The limits of detection (LODs) were in the range of 0.1180-27.82ng/mL for 
      different analytes. The relative standard deviations (RSDs) of inter- and 
      intra-day precisions were less than 3.0% and the recoveries of the assay were in 
      the range of 98.5%-101.5%. The validated method was successfully applied for 
      simultaneous determination of 30 bioactive compounds in XueBiJing injection from 
      10 batches samples by UHPLC-Q-Orbitrap MS within 10min. Moreover, the results 
      were evaluated principal component analysis and two compounds might be the most 
      important chemical markers for chemical quality control of XBJ injection. The 
      novel Q-Orbitrap mass spectrometry has been proved to be a very promising and 
      powerful tool for routine screening of bioactive compounds in traditional Chinese 
      medicine injection, ensuring drug safety and public health.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Zuo, Lihua
AU  - Zuo L
AD  - Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, 
      No.1 Jianshe East Road, Zhengzhou, Henan Province, 450052, PR China.
FAU - Sun, Zhi
AU  - Sun Z
AD  - Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, 
      No.1 Jianshe East Road, Zhengzhou, Henan Province, 450052, PR China. Electronic 
      address: sunzhi2013@163.com.
FAU - Hu, Yurong
AU  - Hu Y
AD  - School of Pharmacy, Zhengzhou University,100 Science Road, Zhengzhou, Henan 
      Province, 450001, PR China.
FAU - Sun, Ya
AU  - Sun Y
AD  - Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, 
      No.1 Jianshe East Road, Zhengzhou, Henan Province, 450052, PR China.
FAU - Xue, Wenhua
AU  - Xue W
AD  - Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, 
      No.1 Jianshe East Road, Zhengzhou, Henan Province, 450052, PR China.
FAU - Zhou, Lin
AU  - Zhou L
AD  - Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, 
      No.1 Jianshe East Road, Zhengzhou, Henan Province, 450052, PR China.
FAU - Zhang, Jun
AU  - Zhang J
AD  - Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, 
      No.1 Jianshe East Road, Zhengzhou, Henan Province, 450052, PR China.
FAU - Bao, Xiaoyue
AU  - Bao X
AD  - Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, 
      No.1 Jianshe East Road, Zhengzhou, Henan Province, 450052, PR China.
FAU - Zhu, Zhenfeng
AU  - Zhu Z
AD  - Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, 
      No.1 Jianshe East Road, Zhengzhou, Henan Province, 450052, PR China.
FAU - Suo, Guanglu
AU  - Suo G
AD  - Department of Ultrasound, The Children's Hospital of Zhengzhou, No.255 Gangdu 
      street, Zhengzhou, Henan Province, 450053, PR China.
FAU - Zhang, Xiaojian
AU  - Zhang X
AD  - Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, 
      No.1 Jianshe East Road, Zhengzhou, Henan Province, 450052, PR China. Electronic 
      address: zhangxiaojian_yxb@163.com.
LA  - eng
PT  - Journal Article
DEP - 20170112
PL  - England
TA  - J Pharm Biomed Anal
JT  - Journal of pharmaceutical and biomedical analysis
JID - 8309336
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Xuebijing)
SB  - IM
MH  - Chromatography, High Pressure Liquid/methods
MH  - Drug Contamination/prevention & control
MH  - Drugs, Chinese Herbal/*analysis/*chemistry
MH  - Injections/methods
MH  - Limit of Detection
MH  - Mass Spectrometry/methods
MH  - Principal Component Analysis/methods
MH  - Quality Control
MH  - Reproducibility of Results
OTO - NOTNLM
OT  - Bioactive constituents
OT  - Quantitative analysis
OT  - UHPLC-Q-Orbitrap MS
OT  - XueBiJing injection
EDAT- 2017/02/02 06:00
MHDA- 2017/06/09 06:00
CRDT- 2017/02/02 06:00
PHST- 2016/09/26 00:00 [received]
PHST- 2016/12/27 00:00 [revised]
PHST- 2017/01/07 00:00 [accepted]
PHST- 2017/02/02 06:00 [pubmed]
PHST- 2017/06/09 06:00 [medline]
PHST- 2017/02/02 06:00 [entrez]
AID - S0731-7085(16)30724-5 [pii]
AID - 10.1016/j.jpba.2017.01.024 [doi]
PST - ppublish
SO  - J Pharm Biomed Anal. 2017 Apr 15;137:220-228. doi: 10.1016/j.jpba.2017.01.024. 
      Epub 2017 Jan 12.