PMID- 28148301
OWN - NLM
STAT- MEDLINE
DCOM- 20171211
LR  - 20190731
IS  - 1471-2474 (Electronic)
IS  - 1471-2474 (Linking)
VI  - 18
IP  - 1
DP  - 2017 Feb 2
TI  - Protective effects of molecular hydrogen on steroid-induced osteonecrosis in 
      rabbits via reducing oxidative stress and apoptosis.
PG  - 58
LID - 10.1186/s12891-017-1431-6 [doi]
LID - 58
AB  - BACKGROUND: The objective of this study was to investigate the protective effects 
      of molecular hydrogen, a novel and selective antioxidant, on steroid-induced 
      osteonecrosis (ON) in a rabbit model. METHODS: Sixty rabbits were randomly 
      divided into two groups (model group and hydrogen group). Osteonecrosis was 
      induced according to an established protocol of steroid-induced ON. Rabbits in 
      the hydrogen group were treated with intraperitoneal injections of molecular 
      hydrogen at 10 ml/kg body weight for seven consecutive days. Plasma levels of 
      total cholesterol, triglycerides, soluble thrombomodulin(sTM), glutathione(GSH) 
      and malondialdehyde(MDA) were measured before and after steroid administration. 
      The presence or absence of ON was examined histopathologically. Oxidative injury 
      and vascular injury were assessed in vivo by immunohistochemical staining of 
      8-hydoxy-2-deoxyguanosine(8-OHdG) and MDA, and ink artery infusion angiography. 
      The terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) 
      assays were performed to measure apoptosis. RESULTS: The incidence of 
      steroid-induced ON was significantly lower in hydrogen group (28.6%) than that in 
      model group (68.0%). No statistically differences were observed on the levels of 
      total cholesterol and triglycerides. Oxidative injury, vascular injury and 
      apoptosis were attenuated in the hydrogen group compared with those in the model 
      group in vivo. CONCLUSIONS: These results suggested that molecular hydrogen 
      prevents steroid-induced osteonecrosis in rabbits by suppressing oxidative 
      injury, vascular injury and apoptosis.
FAU - Li, Jia
AU  - Li J
AD  - Department of Orthopedics, The First Affiliated Hospital of Xi'an Jiaotong 
      University, Yanta West Road, Xi'an, Shaanxi Province, 710061, People's Republic 
      of China. happylee_xjtu@163.com.
FAU - Ge, Zhaogang
AU  - Ge Z
AD  - Department of Joint Surgery, Honghui Hospital of Xi'an Jiaotong University, 
      Xi'an, Shaanxi Province, 710054, People's Republic of China.
FAU - Fan, Lihong
AU  - Fan L
AD  - The first department of Orthopedics, The Second Affiliated Hospital of Xi'an 
      Jiaotong University, Xi'an, Shaanxi Province, 710004, People's Republic of China.
FAU - Wang, Kunzheng
AU  - Wang K
AD  - The first department of Orthopedics, The Second Affiliated Hospital of Xi'an 
      Jiaotong University, Xi'an, Shaanxi Province, 710004, People's Republic of China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170202
PL  - England
TA  - BMC Musculoskelet Disord
JT  - BMC musculoskeletal disorders
JID - 100968565
RN  - 0 (Antioxidants)
RN  - 0 (Glucocorticoids)
RN  - 0 (Thrombomodulin)
RN  - 0 (Triglycerides)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - 7YNJ3PO35Z (Hydrogen)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - GAN16C9B8O (Glutathione)
RN  - X4W7ZR7023 (Methylprednisolone)
SB  - IM
MH  - Angiography
MH  - Animals
MH  - Antioxidants/*pharmacology
MH  - Apoptosis/*drug effects
MH  - Cholesterol/blood
MH  - Disease Models, Animal
MH  - Glucocorticoids/*toxicity
MH  - Glutathione/blood
MH  - Humans
MH  - Hydrogen/*pharmacology
MH  - Immunohistochemistry
MH  - In Situ Nick-End Labeling
MH  - Incidence
MH  - Injections, Intraperitoneal
MH  - Male
MH  - Malondialdehyde/blood
MH  - Methylprednisolone/toxicity
MH  - Osteonecrosis/blood/chemically induced/epidemiology/*prevention & control
MH  - Oxidative Stress/*drug effects
MH  - Rabbits
MH  - Random Allocation
MH  - Thrombomodulin/blood
MH  - Triglycerides/blood
PMC - PMC5288900
OTO - NOTNLM
OT  - Apoptosis
OT  - Molecular hydrogen
OT  - Osteonecrosis
OT  - Oxidative injury
OT  - Vascular injury
EDAT- 2017/02/06 06:00
MHDA- 2017/12/12 06:00
PMCR- 2017/02/02
CRDT- 2017/02/03 06:00
PHST- 2016/06/09 00:00 [received]
PHST- 2017/01/24 00:00 [accepted]
PHST- 2017/02/03 06:00 [entrez]
PHST- 2017/02/06 06:00 [pubmed]
PHST- 2017/12/12 06:00 [medline]
PHST- 2017/02/02 00:00 [pmc-release]
AID - 10.1186/s12891-017-1431-6 [pii]
AID - 1431 [pii]
AID - 10.1186/s12891-017-1431-6 [doi]
PST - epublish
SO  - BMC Musculoskelet Disord. 2017 Feb 2;18(1):58. doi: 10.1186/s12891-017-1431-6.