PMID- 28150359
OWN - NLM
STAT- MEDLINE
DCOM- 20170327
LR  - 20170327
IS  - 1095-8355 (Electronic)
IS  - 1065-6995 (Linking)
VI  - 41
IP  - 4
DP  - 2017 Apr
TI  - Potential role of tracing stem cell transplantation and effects on the immune 
      cell function of ferumoxytol combining with heparin and protamine in vivo/in 
      vitro.
PG  - 423-432
LID - 10.1002/cbin.10737 [doi]
AB  - Cell labeling and tracing have played an increasingly important role in the field 
      of stem cell transplantation. Nanocomplexes combining three Food and Drug 
      Administration (FDA)-approved drugs: heparin (H), protamine (P), and ferumoxytol 
      (F) (HPF nanocomplexes) display high labeling efficiency in human adipose 
      tissue-derived stem cells (hADSCs), but their biological safety has not been 
      determined. In this study, we tested the labeling efficiency of HPF in hADSCs 
      through in vitro cytotoxicity studies and in vivo murine preclinical studies 
      using HPF-labeled hADSCs. The labeling process did not cause cell apoptosis and 
      had little effect on cell proliferation. In vivo magnetic resonance imaging (MRI) 
      showed that the HPF-labeled cells produced a hypointense signal that did not 
      affect liver and kidney functions. However, after injection of HPF-labeled cells 
      into mice, lymphocyte transformation testing showed that T and B lymphocyte 
      proliferation was significantly increased. These findings suggest that extensive 
      safety testing of HPF nanocomplexes is necessary; the process to evaluate HPF as 
      an investigative new drug application could therefore be postponed.
CI  - (c) 2017 International Federation for Cell Biology.
FAU - Zhao, Jingyuan
AU  - Zhao J
AD  - Regenerative Medicine Center, The First Affiliated Hospital of Dalian Medical 
      University, 222 Zhongshan Road, Dalian, 116011, China.
FAU - Guan, Xin
AU  - Guan X
AD  - Regenerative Medicine Center, The First Affiliated Hospital of Dalian Medical 
      University, 222 Zhongshan Road, Dalian, 116011, China.
FAU - Liu, Yang
AU  - Liu Y
AD  - Regenerative Medicine Center, The First Affiliated Hospital of Dalian Medical 
      University, 222 Zhongshan Road, Dalian, 116011, China.
FAU - Piao, Hua
AU  - Piao H
AD  - College of Basic Medical Sciences, Dalian Medical University, Dalian, China.
FAU - Liu, Rutao
AU  - Liu R
AD  - Regenerative Medicine Center, The First Affiliated Hospital of Dalian Medical 
      University, 222 Zhongshan Road, Dalian, 116011, China.
FAU - Zhou, Xiang
AU  - Zhou X
AD  - Regenerative Medicine Center, The First Affiliated Hospital of Dalian Medical 
      University, 222 Zhongshan Road, Dalian, 116011, China.
FAU - Sun, Bo
AU  - Sun B
AD  - Department of Radiology, The First Affiliated Hospital of Dalian Medical 
      University, Dalian, China.
FAU - Du, Yue
AU  - Du Y
AD  - College of Basic Medical Sciences, Dalian Medical University, Dalian, China.
FAU - Liu, Jing
AU  - Liu J
AD  - Regenerative Medicine Center, The First Affiliated Hospital of Dalian Medical 
      University, 222 Zhongshan Road, Dalian, 116011, China.
LA  - eng
PT  - Journal Article
DEP - 20170220
PL  - England
TA  - Cell Biol Int
JT  - Cell biology international
JID - 9307129
RN  - 0 (Protamines)
RN  - 9005-49-6 (Heparin)
RN  - XM0M87F357 (Ferrosoferric Oxide)
SB  - IM
MH  - Adipose Tissue/cytology
MH  - Adult
MH  - Apoptosis
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - Cell Shape
MH  - Cell Survival
MH  - Cell Tracking
MH  - Cells, Cultured
MH  - Female
MH  - Ferrosoferric Oxide/*chemistry/toxicity
MH  - Heparin/*chemistry/toxicity
MH  - Humans
MH  - Magnetic Resonance Imaging
MH  - Middle Aged
MH  - Nanoparticles/chemistry/toxicity
MH  - Protamines/*chemistry/toxicity
MH  - Staining and Labeling
MH  - Stem Cell Transplantation
MH  - Stem Cells/physiology
OTO - NOTNLM
OT  - MRI
OT  - cell labeling
OT  - ferumoxytol
OT  - lymphocyte proliferation
OT  - stem cells
EDAT- 2017/02/06 06:00
MHDA- 2017/03/28 06:00
CRDT- 2017/02/03 06:00
PHST- 2016/09/29 00:00 [received]
PHST- 2017/01/31 00:00 [accepted]
PHST- 2017/02/06 06:00 [pubmed]
PHST- 2017/03/28 06:00 [medline]
PHST- 2017/02/03 06:00 [entrez]
AID - 10.1002/cbin.10737 [doi]
PST - ppublish
SO  - Cell Biol Int. 2017 Apr;41(4):423-432. doi: 10.1002/cbin.10737. Epub 2017 Feb 20.