PMID- 28151970
OWN - NLM
STAT- MEDLINE
DCOM- 20170814
LR  - 20221207
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 2
DP  - 2017
TI  - Establishing a reference interval for serum anti-dsDNA antibody: A large Chinese 
      Han population-based multi-center study.
PG  - e0168871
LID - 10.1371/journal.pone.0168871 [doi]
LID - e0168871
AB  - BACKGROUND: A reference interval (RI) for the circulating concentration of 
      anti-dsDNA antibody is essential for clinicians to interpret laboratory results 
      and make clinical decisions. Therefore, we aimed to establish the RI for 
      anti-dsDNA antibody in the Chinese Han population. METHODS: This study was 
      designed and carried out in accordance with guideline C28-A3, which is proposed 
      by the International Federation of Clinical Chemistry and the Clinical and 
      Laboratory Standards Institute. A total of 2,880 apparently healthy individuals 
      were enrolled using a posteriori sampling. These individuals were recruited from 
      four hospitals, representing the Han populations of north, south, east, and west 
      China. Serum anti-dsDNA antibody levels were measured using the three analytical 
      systems AESKU, EUROIMMUNE, and INOVA, which are the most commonly used systems in 
      China. Individuals were stratified by gender, age, and region, and the RIs were 
      obtained by nonparametric methods. RESULTS: Gender-specific RIs for serum 
      anti-dsDNA antibody in the Chinese Han population were established. CONCLUSION: 
      This is the first exploration of the RI for anti-dsDNA antibody in the Chinese 
      Han population. We have established gender-specific RIs for each assay method 
      commonly used in China.
FAU - Deng, Chuiwen
AU  - Deng C
AD  - Department of Rheumatology and Clinical Immunology, Peking Union Medical College 
      Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, 
      Beijing, China.
AD  - Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, 
      Beijing, China.
FAU - Zhang, Shulan
AU  - Zhang S
AD  - Department of Rheumatology and Clinical Immunology, Peking Union Medical College 
      Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, 
      Beijing, China.
AD  - Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, 
      Beijing, China.
FAU - Hu, Chaojun
AU  - Hu C
AD  - Department of Rheumatology and Clinical Immunology, Peking Union Medical College 
      Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, 
      Beijing, China.
AD  - Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, 
      Beijing, China.
FAU - Li, Ping
AU  - Li P
AD  - Department of Rheumatology and Clinical Immunology, Peking Union Medical College 
      Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, 
      Beijing, China.
AD  - Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, 
      Beijing, China.
FAU - Wu, Ziyan
AU  - Wu Z
AD  - Department of Rheumatology and Clinical Immunology, Peking Union Medical College 
      Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, 
      Beijing, China.
AD  - Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, 
      Beijing, China.
FAU - Chen, Si
AU  - Chen S
AD  - Department of Rheumatology and Clinical Immunology, Peking Union Medical College 
      Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, 
      Beijing, China.
AD  - Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, 
      Beijing, China.
FAU - Li, Jing
AU  - Li J
AD  - Department of Rheumatology and Clinical Immunology, Peking Union Medical College 
      Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, 
      Beijing, China.
AD  - Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, 
      Beijing, China.
FAU - Li, Liubing
AU  - Li L
AD  - Department of Rheumatology and Clinical Immunology, Peking Union Medical College 
      Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, 
      Beijing, China.
AD  - Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, 
      Beijing, China.
FAU - Zhang, Fengchun
AU  - Zhang F
AD  - Department of Rheumatology and Clinical Immunology, Peking Union Medical College 
      Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, 
      Beijing, China.
AD  - Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, 
      Beijing, China.
FAU - Li, Yongzhe
AU  - Li Y
AD  - Department of Rheumatology and Clinical Immunology, Peking Union Medical College 
      Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, 
      Beijing, China.
AD  - Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, 
      Beijing, China.
LA  - eng
PT  - Journal Article
PT  - Multicenter Study
DEP - 20170202
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Antibodies, Antinuclear)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Antibodies, Antinuclear/*blood
MH  - Asian People
MH  - China
MH  - Ethnicity
MH  - Female
MH  - Healthy Volunteers
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Reference Values
MH  - Young Adult
PMC - PMC5289441
COIS- The authors have declared that no competing interests exist.
EDAT- 2017/02/06 06:00
MHDA- 2017/08/15 06:00
PMCR- 2017/02/02
CRDT- 2017/02/03 06:00
PHST- 2016/07/27 00:00 [received]
PHST- 2016/12/07 00:00 [accepted]
PHST- 2017/02/03 06:00 [entrez]
PHST- 2017/02/06 06:00 [pubmed]
PHST- 2017/08/15 06:00 [medline]
PHST- 2017/02/02 00:00 [pmc-release]
AID - PONE-D-16-29873 [pii]
AID - 10.1371/journal.pone.0168871 [doi]
PST - epublish
SO  - PLoS One. 2017 Feb 2;12(2):e0168871. doi: 10.1371/journal.pone.0168871. 
      eCollection 2017.