PMID- 28153781
OWN - NLM
STAT- MEDLINE
DCOM- 20170530
LR  - 20171209
IS  - 1090-2422 (Electronic)
IS  - 0014-4827 (Linking)
VI  - 352
IP  - 1
DP  - 2017 Mar 1
TI  - Msx1-modulated muscle satellite cells retain a primitive state and exhibit an 
      enhanced capacity for osteogenic differentiation.
PG  - 84-94
LID - S0014-4827(17)30027-7 [pii]
LID - 10.1016/j.yexcr.2017.01.016 [doi]
AB  - Multipotent muscle satellite cells (MuSCs) have been identified as potential seed 
      cells for bone tissue engineering. However, MuSCs exhibit a rapid loss of 
      stemness after in vitro culturing, thereby compromising their therapeutic 
      efficiency. Muscle segment homeobox gene 1 (msx1) has been found to induce the 
      dedifferentiation of committed progenitor cells, as well as terminally 
      differentiated myotubes. In this study, a Tet-off retroviral gene delivery system 
      was used to modulate msx1 expression. After ten passages, MuSCs that did not 
      express msx-1 (e.g., the non-msx1 group) were compared with MuSCs with induced 
      msx-1 expression (e.g., the msx1 group). The latter group exhibited a more 
      juvenile morphology, it contained a significantly lower percentage of senescent 
      cells characterized by positive beta-galactosidase staining, and it exhibited 
      increased proliferation and a higher proliferation index. Immunocytochemical 
      stainings further detected a more primitive gene expression profile for the msx1 
      group, while osteogenic differentiation assays and ectopic bone formation assays 
      demonstrated an improved capacity for the msx1 group to undergo osteogenic 
      differentiation. These results suggest that transient expression of msx1 in MuSCs 
      can retain a primitive state, thereby enhancing their capacity for osteogenic 
      differentiation and restoring the potential for MuSCs to serve as seed cells for 
      bone tissue engineering.
CI  - Copyright (c) 2017 Elsevier Inc. All rights reserved.
FAU - Ding, Ke
AU  - Ding K
AD  - Department of Pediatric Surgery, School of medicine, University of Electronic 
      Science and Technology of China, Chengdu 610072, China; Sichuan Academy of 
      Medical Sciences & Sichuan Provincial People's Hospital, Chengdu 610072, China; 
      Department of Orthopaedics, Southwest Hospital, Third Military Medical 
      University, Chongqing 400038, China. Electronic address: dingke@med.uestc.edu.cn.
FAU - Liu, Wen-Ying
AU  - Liu WY
AD  - Department of Pediatric Surgery, School of medicine, University of Electronic 
      Science and Technology of China, Chengdu 610072, China; Sichuan Academy of 
      Medical Sciences & Sichuan Provincial People's Hospital, Chengdu 610072, China.
FAU - Zeng, Qiang
AU  - Zeng Q
AD  - Department of Pediatric Surgery, School of medicine, University of Electronic 
      Science and Technology of China, Chengdu 610072, China; Sichuan Academy of 
      Medical Sciences & Sichuan Provincial People's Hospital, Chengdu 610072, China.
FAU - Hou, Fang
AU  - Hou F
AD  - Department of Pediatric Surgery, School of medicine, University of Electronic 
      Science and Technology of China, Chengdu 610072, China; Sichuan Academy of 
      Medical Sciences & Sichuan Provincial People's Hospital, Chengdu 610072, China.
FAU - Xu, Jian-Zhong
AU  - Xu JZ
AD  - Department of Orthopaedics, Southwest Hospital, Third Military Medical 
      University, Chongqing 400038, China. Electronic address: xjzspine@163.com.
FAU - Yang, Zhong
AU  - Yang Z
AD  - Department of Clinical Hematology, Southwest Hospital, Third Military Medical 
      University, Chongqing 400038, China. Electronic address: zyang1999@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170131
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (MSX1 Transcription Factor)
RN  - 0 (RNA, Messenger)
RN  - EC 3.2.1.23 (beta-Galactosidase)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Blotting, Western
MH  - *Cell Differentiation
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - MSX1 Transcription Factor/genetics/*metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Nude
MH  - Mice, Transgenic
MH  - Osteogenesis/*physiology
MH  - RNA, Messenger/genetics
MH  - Real-Time Polymerase Chain Reaction
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Satellite Cells, Skeletal Muscle/*cytology/metabolism/transplantation
MH  - beta-Galactosidase
OTO - NOTNLM
OT  - Bone tissue engineering
OT  - Msx1
OT  - Multipotential
OT  - Muscle satellite cells
OT  - Osteogenic differentiation
EDAT- 2017/02/06 06:00
MHDA- 2017/05/31 06:00
CRDT- 2017/02/04 06:00
PHST- 2016/09/07 00:00 [received]
PHST- 2017/01/03 00:00 [revised]
PHST- 2017/01/29 00:00 [accepted]
PHST- 2017/02/06 06:00 [pubmed]
PHST- 2017/05/31 06:00 [medline]
PHST- 2017/02/04 06:00 [entrez]
AID - S0014-4827(17)30027-7 [pii]
AID - 10.1016/j.yexcr.2017.01.016 [doi]
PST - ppublish
SO  - Exp Cell Res. 2017 Mar 1;352(1):84-94. doi: 10.1016/j.yexcr.2017.01.016. Epub 
      2017 Jan 31.