PMID- 28154457
OWN - NLM
STAT- MEDLINE
DCOM- 20180731
LR  - 20181113
IS  - 1928-9022 (Electronic)
IS  - 0830-9000 (Print)
IS  - 0830-9000 (Linking)
VI  - 81
IP  - 1
DP  - 2017 Jan
TI  - Evaluation of the new commercial recombinant chimeric subunit vaccine PRRSFREE in 
      challenge with heterologous types 1 and 2 porcine reproductive and respiratory 
      syndrome virus.
PG  - 12-21
AB  - The objective of this study was to evaluate a new recombinant chimeric vaccine 
      against porcine reproductive and respiratory syndrome virus (PRRSV). The subunit 
      vaccine, PRRSFREE, from Reber Genetics, Taiwan, Republic of China, is based on a 
      plasmid containing a detoxified Pseudomonas exotoxin carrying open reading frame 
      (ORF) 7, 1b, and 5 and 6 chimeric subunits of types 1 and 2 PRRSV. Pigs were 
      injected intramuscularly with 2.0 mL of the vaccine at 21 and 42 d of age, 
      according to the manufacturer's recommendation. At the age of 63 d the pigs were 
      inoculated intranasally with either type 1 or type 2 PRRSV. Regardless of the 
      genotype of the challenging PRRSV, the vaccinated challenged pigs had 
      significantly lower (P < 0.05) mean rectal temperature, respiratory score, lung 
      lesion score, and amount of PRRSV antigen within areas of interstitial pneumonia, 
      along with overall lower levels of viremia due to type 1 or type 2 PRRSV compared 
      with the unvaccinated challenged pigs. The vaccinated challenged pigs also had 
      significantly higher (P < 0.05) numbers of interferon-gamma secreting cells compared 
      with the unvaccinated challenged pigs. This study demonstrated that the new 
      vaccine provides protection against respiratory disease from heterologous types 1 
      and 2 PRRSV challenge in growing pigs.
FAU - Jeong, Jiwoon
AU  - Jeong J
AD  - Department of Veterinary Pathology, College of Veterinary Medicine, Seoul 
      National University, 1 Gwanak-ro, Gwanak-gu, 151-742, Seoul, Republic of Korea.
FAU - Park, Changhoon
AU  - Park C
AD  - Department of Veterinary Pathology, College of Veterinary Medicine, Seoul 
      National University, 1 Gwanak-ro, Gwanak-gu, 151-742, Seoul, Republic of Korea.
FAU - Choi, Kyuhyung
AU  - Choi K
AD  - Department of Veterinary Pathology, College of Veterinary Medicine, Seoul 
      National University, 1 Gwanak-ro, Gwanak-gu, 151-742, Seoul, Republic of Korea.
FAU - Chae, Chanhee
AU  - Chae C
AD  - Department of Veterinary Pathology, College of Veterinary Medicine, Seoul 
      National University, 1 Gwanak-ro, Gwanak-gu, 151-742, Seoul, Republic of Korea.
LA  - eng
PT  - Journal Article
PT  - Randomized Controlled Trial
PL  - Canada
TA  - Can J Vet Res
JT  - Canadian journal of veterinary research = Revue canadienne de recherche 
      veterinaire
JID - 8607793
RN  - 0 (Antibodies, Viral)
RN  - 0 (RNA, Viral)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Vaccines, Subunit)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - Lung/virology
MH  - Porcine Reproductive and Respiratory Syndrome/*prevention & control
MH  - Porcine respiratory and reproductive syndrome virus/*classification
MH  - RNA, Viral
MH  - Recombinant Proteins/*immunology
MH  - Swine
MH  - Vaccines, Subunit/immunology
MH  - Viral Vaccines/*immunology
MH  - Viremia
PMC - PMC5220588
EDAT- 2017/02/06 06:00
MHDA- 2018/08/01 06:00
PMCR- 2017/07/01
CRDT- 2017/02/04 06:00
PHST- 2017/02/04 06:00 [entrez]
PHST- 2017/02/06 06:00 [pubmed]
PHST- 2018/08/01 06:00 [medline]
PHST- 2017/07/01 00:00 [pmc-release]
AID - cjvr_01_12 [pii]
PST - ppublish
SO  - Can J Vet Res. 2017 Jan;81(1):12-21.