PMID- 28157196
OWN - NLM
STAT- MEDLINE
DCOM- 20181024
LR  - 20181113
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 7
DP  - 2017 Feb 3
TI  - Effect of Different Titanium Surfaces on Maturation of Murine Bone Marrow-Derived 
      Dendritic Cells.
PG  - 41945
LID - 10.1038/srep41945 [doi]
LID - 41945
AB  - Dendritic cells (DCs) play a pivotal role in the host response to implanted 
      biomaterials. Osseointegration of titanium (Ti) implant is an immunological and 
      inflammatory-driven process. However, the role of DCs in this complex process is 
      largely unknown. This study aimed to investigate the effect of different Ti 
      surfaces on DC maturation, and evaluate its subsequent potential on osteogenic 
      differentiation of preosteoblasts. Murine bone marrow-derived DCs were seeded on 
      Ti disks with different surface treatments, including pretreatment (PT), 
      sandblasted/acid-etched (SLA) and modified SLA (modSLA) surface. Compared with 
      DCs cultured on PT and SLA surfaces, the cells seeded on modSLA surface 
      demonstrated a more round morphology with lower expression of CD86 and MHC-II, 
      the DC maturation markers. Those cells also secreted high levels of 
      anti-inflammatory cytokine IL-10 and TGF-beta. Notably, addition of conditioned 
      medium (CM) from modSLA-induced DCs significantly increased the mRNA expression 
      of Runx2 and ALP as well as ALP activity by murine preosteoblast MC3T3-E1 cells. 
      Our data demonstrated that Ti disks with different surfaces lead to differential 
      DCs responses. PT and SLA surfaces induce DCs mature, while DCs seeded on 
      modSLA-Ti surface maintain an immature phenotype and exhibit a potential of 
      promoting osteogenic differentiation of MC3T3-E1 cells.
FAU - Zheng, Xiaofei
AU  - Zheng X
AD  - State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, 
      Sichuan University, Chengdu, Sichuan, China.
AD  - Dental Implant Center, West China Hospital of Stomatology, Sichuan University, 
      Chengdu, Sichuan, China.
FAU - Zhou, Fengjuan
AU  - Zhou F
AD  - State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, 
      Sichuan University, Chengdu, Sichuan, China.
AD  - Geriatric Dentistry Department, West China Hospital of Stomatology, Sichuan 
      University, Chengdu, Sichuan, China.
FAU - Gu, Yifei
AU  - Gu Y
AD  - State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, 
      Sichuan University, Chengdu, Sichuan, China.
AD  - Dental Implant Center, West China Hospital of Stomatology, Sichuan University, 
      Chengdu, Sichuan, China.
FAU - Duan, Xiaobo
AU  - Duan X
AD  - State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, 
      Sichuan University, Chengdu, Sichuan, China.
AD  - Dental Implant Center, West China Hospital of Stomatology, Sichuan University, 
      Chengdu, Sichuan, China.
FAU - Mo, Anchun
AU  - Mo A
AD  - State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, 
      Sichuan University, Chengdu, Sichuan, China.
AD  - Dental Implant Center, West China Hospital of Stomatology, Sichuan University, 
      Chengdu, Sichuan, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170203
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (B7-2 Antigen)
RN  - 0 (Core Binding Factor Alpha 1 Subunit)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Runx2 protein, mouse)
RN  - 0 (Transforming Growth Factor beta)
RN  - 130068-27-8 (Interleukin-10)
RN  - D1JT611TNE (Titanium)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/genetics/metabolism
MH  - Animals
MH  - B7-2 Antigen/genetics/metabolism
MH  - Bone Marrow Cells/cytology/drug effects
MH  - *Cell Differentiation
MH  - Cell Line
MH  - Cells, Cultured
MH  - Core Binding Factor Alpha 1 Subunit/genetics/metabolism
MH  - Dendritic Cells/*cytology/drug effects/metabolism
MH  - Histocompatibility Antigens Class II/genetics/metabolism
MH  - Interleukin-10/genetics/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Titanium/*pharmacology
MH  - Transforming Growth Factor beta/genetics/metabolism
PMC - PMC5291203
COIS- The authors declare no competing financial interests.
EDAT- 2017/02/06 06:00
MHDA- 2018/10/26 06:00
PMCR- 2017/02/03
CRDT- 2017/02/04 06:00
PHST- 2016/07/08 00:00 [received]
PHST- 2016/12/28 00:00 [accepted]
PHST- 2017/02/04 06:00 [entrez]
PHST- 2017/02/06 06:00 [pubmed]
PHST- 2018/10/26 06:00 [medline]
PHST- 2017/02/03 00:00 [pmc-release]
AID - srep41945 [pii]
AID - 10.1038/srep41945 [doi]
PST - epublish
SO  - Sci Rep. 2017 Feb 3;7:41945. doi: 10.1038/srep41945.