PMID- 28163600
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1344-7610 (Print)
IS  - 1347-3735 (Electronic)
IS  - 1344-7610 (Linking)
VI  - 66
IP  - 5
DP  - 2016 Dec
TI  - Cytological characterization of an interspecific hybrid in Jatropha and its 
      progeny reveals preferential uniparental chromosome transmission and 
      interspecific translocation.
PG  - 838-844
LID - 10.1270/jsbbs.16069 [doi]
AB  - Genetic variation in Jatropha curcas, a prospective biodiesel plant, is limited, 
      and interspecific hybridization needed for its genetic improvement. Progeny from 
      interspecific crosses between J. curcas and Jatropha integerrima can be used to 
      improve agronomic characters and to increase oil content and yield. However, 
      these hybrids have not been characterized cytologically. The present study was 
      aimed at the analysis of chromosome behavior during meiosis and chromosome 
      composition of S(1) plants derived from an interspecific F(1) hybrid using 
      genomic in situ hybridization (GISH) and fluorescence in situ hybridization 
      (FISH). Bivalents that formed as a result of interspecific pairing were 
      frequently observed, suggesting the presence of homoeologous chromosomes from the 
      two species. Almost half of microspores were derived from the reduction division; 
      GISH analysis indicated random transmission of the parent chromosomes to 
      microspores. Male fertility measured as pollen staining with acetocarmine was 
      48.4%. In contrast, GISH analysis of S(1) plants revealed preferential 
      transmission of J. curcas chromosomes. We also found segment exchange between 
      chromosomes of the two species (interspecific translocation) by GISH and FISH 
      analyses. Introgression of J. integerrima chromosome segments into the J. curcas 
      genome would help to improve Jatropha cultivars for mass production.
FAU - Fukuhara, Shuto
AU  - Fukuhara S
AD  - Laboratory of Genetics and Plant Breeding, Graduate School of Horticulture, Chiba 
      University , Matsudo 648, Chiba 271-8510 , Japan.
FAU - Muakrong, Narathid
AU  - Muakrong N
AD  - Laboratory of Genetics and Plant Breeding, Graduate School of Horticulture, Chiba 
      University, Matsudo 648, Chiba 271-8510, Japan; Faculty of Agriculture, Princess 
      Naradhiwas University, Narathiwat 96110, Thailand.
FAU - Kikuchi, Shinji
AU  - Kikuchi S
AD  - Laboratory of Genetics and Plant Breeding, Graduate School of Horticulture, Chiba 
      University , Matsudo 648, Chiba 271-8510 , Japan.
FAU - Tanya, Patcharin
AU  - Tanya P
AD  - Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart 
      University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand; Center for Advanced 
      Studies for Agriculture and Food, Kasetsart University Institute for Advanced 
      Studies, Kasetsart University, Bangkok 10900 Thailand (CASAF, NRU-KU, Thailand).
FAU - Sassa, Hidenori
AU  - Sassa H
AD  - Laboratory of Genetics and Plant Breeding, Graduate School of Horticulture, Chiba 
      University , Matsudo 648, Chiba 271-8510 , Japan.
FAU - Koba, Takato
AU  - Koba T
AD  - Laboratory of Genetics and Plant Breeding, Graduate School of Horticulture, Chiba 
      University , Matsudo 648, Chiba 271-8510 , Japan.
FAU - Srinives, Peerasak
AU  - Srinives P
AD  - Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart 
      University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand; Center for Advanced 
      Studies for Agriculture and Food, Kasetsart University Institute for Advanced 
      Studies, Kasetsart University, Bangkok 10900 Thailand (CASAF, NRU-KU, Thailand).
LA  - eng
PT  - Journal Article
DEP - 20161019
PL  - Japan
TA  - Breed Sci
JT  - Breeding science
JID - 9888571
PMC - PMC5282757
OTO - NOTNLM
OT  - Jatropha curcas
OT  - Jatropha integerrima
OT  - fluorescence in situ hybridization (FISH)
OT  - genomic in situ hybridization (GISH)
OT  - interspecific hybrid
OT  - interspecific translocation
OT  - preferential transmission
EDAT- 2017/02/07 06:00
MHDA- 2017/02/07 06:01
PMCR- 2016/12/01
CRDT- 2017/02/07 06:00
PHST- 2016/04/05 00:00 [received]
PHST- 2016/08/22 00:00 [accepted]
PHST- 2017/02/07 06:00 [entrez]
PHST- 2017/02/07 06:00 [pubmed]
PHST- 2017/02/07 06:01 [medline]
PHST- 2016/12/01 00:00 [pmc-release]
AID - 66_16069 [pii]
AID - 10.1270/jsbbs.16069 [doi]
PST - ppublish
SO  - Breed Sci. 2016 Dec;66(5):838-844. doi: 10.1270/jsbbs.16069. Epub 2016 Oct 19.