PMID- 28165374
OWN - NLM
STAT- MEDLINE
DCOM- 20170420
LR  - 20220129
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 18
IP  - 2
DP  - 2017 Feb 3
TI  - Evaluation of EphA2 and EphB4 as Targets for Image-Guided Colorectal Cancer 
      Surgery.
LID - 10.3390/ijms18020307 [doi]
LID - 307
AB  - Targeted image-guided oncologic surgery (IGOS) relies on the recognition of cell 
      surface-associated proteins, which should be abundantly present on tumor cells 
      but preferably absent on cells in surrounding healthy tissue. The transmembrane 
      receptor tyrosine kinase EphA2, a member of the A class of the Eph receptor 
      family, has been reported to be highly overexpressed in several tumor types 
      including breast, lung, brain, prostate, and colon cancer and is considered 
      amongst the most promising cell membrane-associated tumor antigens by the NIH. 
      Another member of the Eph receptor family belonging to the B class, EphB4, has 
      also been found to be upregulated in multiple cancer types. In this study, EphA2 
      and EphB4 are evaluated as targets for IGOS of colorectal cancer by 
      immunohistochemistry (IHC) using a tissue microarray (TMA) consisting of 168 
      pairs of tumor and normal tissue. The IHC sections were scored for staining 
      intensity and percentage of cells stained. The results show a significantly 
      enhanced staining intensity and more widespread distribution in tumor tissue 
      compared with adjacent normal tissue for EphA2 as well as EphB4. Based on its 
      more consistently higher score in colorectal tumor tissue compared to normal 
      tissue, EphB4 appears to be a promising candidate for IGOS of colorectal cancer. 
      In vitro experiments using antibodies on human colon cancer cells confirmed the 
      possibility of EphB4 as target for imaging.
FAU - Stammes, Marieke A
AU  - Stammes MA
AD  - Department of Radiology, Leiden University Medical Center, 2300 RC Leiden, The 
      Netherlands. m.a.stammes@lumc.nl.
AD  - Percuros B.V., 2333 CL Leiden, The Netherlands. m.a.stammes@lumc.nl.
FAU - Prevoo, Hendrica A J M
AU  - Prevoo HA
AD  - Department of Surgery, Leiden University Medical Center, 2300 RC Leiden, The 
      Netherlands. h.a.j.m.prevoo@lumc.nl.
FAU - Ter Horst, Meyke C
AU  - Ter Horst MC
AD  - Department of Surgery, Leiden University Medical Center, 2300 RC Leiden, The 
      Netherlands. m.c.terhorst@lumc.nl.
FAU - Groot, Stephanie A
AU  - Groot SA
AD  - Department of Surgery, Leiden University Medical Center, 2300 RC Leiden, The 
      Netherlands. s.a.groot@lumc.nl.
FAU - Van de Velde, Cornelis J H
AU  - Van de Velde CJ
AD  - Department of Surgery, Leiden University Medical Center, 2300 RC Leiden, The 
      Netherlands. c.j.h.vandevelde@lumc.nl.
FAU - Chan, Alan B
AU  - Chan AB
AD  - Percuros B.V., 2333 CL Leiden, The Netherlands. achan@percuros.com.
FAU - de Geus-Oei, Lioe-Fee
AU  - de Geus-Oei LF
AD  - Department of Radiology, Leiden University Medical Center, 2300 RC Leiden, The 
      Netherlands.
FAU - Kuppen, Peter J K
AU  - Kuppen PJ
AD  - Department of Surgery, Leiden University Medical Center, 2300 RC Leiden, The 
      Netherlands. p.j.k.kuppen@lumc.nl.
FAU - Vahrmeijer, Alexander L
AU  - Vahrmeijer AL
AD  - Department of Surgery, Leiden University Medical Center, 2300 RC Leiden, The 
      Netherlands. a.l.vahrmeijer@lumc.nl.
FAU - Pasquale, Elena B
AU  - Pasquale EB
AD  - Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA 92037, USA. 
      elenap@sbpdiscovery.org.
FAU - Sier, Cornelis F M
AU  - Sier CF
AD  - Department of Surgery, Leiden University Medical Center, 2300 RC Leiden, The 
      Netherlands. c.f.m.sier@lumc.nl.
AD  - Antibodies for Research Applications B.V., 2805 HT Gouda, The Netherlands. 
      c.f.m.sier@lumc.nl.
LA  - eng
GR  - 323105/ERC_/European Research Council/International
GR  - P01 CA138390/CA/NCI NIH HHS/United States
PT  - Journal Article
DEP - 20170203
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Biomarkers)
RN  - EC 2.7.10.1 (Receptor, EphA2)
RN  - EC 2.7.10.1 (Receptor, EphA4)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Biomarkers
MH  - Cell Line, Tumor
MH  - Colorectal Neoplasms/diagnosis/*metabolism/*surgery
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Grading
MH  - Neoplasm Staging
MH  - Receptor, EphA2/*metabolism
MH  - Receptor, EphA4/*metabolism
MH  - *Surgery, Computer-Assisted/methods
PMC - PMC5343843
OTO - NOTNLM
OT  - cancer imaging
OT  - colon cancer
OT  - immunohistochemistry
OT  - normal tissue
OT  - tissue microarray
OT  - tyrosine kinase receptor
COIS- The authors declare no conflict of interest. The founding sponsors had no role in 
      the design of the study; in the collection, analyses, or interpretation of data; 
      in the writing of the manuscript; or in the decision to publish the results.
EDAT- 2017/02/07 06:00
MHDA- 2017/04/21 06:00
PMCR- 2017/02/01
CRDT- 2017/02/07 06:00
PHST- 2016/11/23 00:00 [received]
PHST- 2016/12/30 00:00 [revised]
PHST- 2017/01/21 00:00 [accepted]
PHST- 2017/02/07 06:00 [entrez]
PHST- 2017/02/07 06:00 [pubmed]
PHST- 2017/04/21 06:00 [medline]
PHST- 2017/02/01 00:00 [pmc-release]
AID - ijms18020307 [pii]
AID - ijms-18-00307 [pii]
AID - 10.3390/ijms18020307 [doi]
PST - epublish
SO  - Int J Mol Sci. 2017 Feb 3;18(2):307. doi: 10.3390/ijms18020307.