PMID- 28165889 OWN - NLM STAT- MEDLINE DCOM- 20170808 LR - 20181202 IS - 1544-0591 (Electronic) IS - 0022-0345 (Linking) VI - 96 IP - 6 DP - 2017 Jun TI - Force-Induced H(2)S by PDLSCs Modifies Osteoclastic Activity during Tooth Movement. PG - 694-702 LID - 10.1177/0022034517690388 [doi] AB - Hydrogen sulfide (H(2)S), a gasotransmitter, has been recently linked to mesenchymal stem cell (MSC) function and bone homeostasis. Periodontal ligament stem cells (PDLSCs) are the main MSCs in PDL, which respond to mechanical force to induce physiological activities during orthodontic tooth movement (OTM). However, it is unknown whether mechanical force might induce endogenous H(2)S production by PDLSCs to regulate alveolar bone homeostasis. Here, we used a mouse OTM model to demonstrate that orthodontic force-induced endogenous H(2)S production in PDL tissue was associated with macrophage accumulation and osteoclastic activity in alveolar bone. Then, we showed that mechanical force application induced cystathionine beta-synthase (CBS) expression and endogenous H(2)S production by PDLSCs. Moreover, blocking endogenous H(2)S or systemically increasing H(2)S levels could decrease or enhance force-induced osteoclastic activities to control tooth movement. We further revealed how force-induced H(2)S production by PDLSCs contributed to the secretion of monocyte chemoattractant protein-1 (MCP-1) and the expression of receptor activator of nuclear factor-kappaB ligand/osteoprotegerin (RANKL/OPG) system by PDLSCs. The secretion and expression of these factors controlled macrophage migration and osteoclast differentiation. This study demonstrated that PDLSCs produced H(2)S to respond to and transduce force signals. Force-induced gasotransmitter H(2)S production in PDLSCs therefore regulated osteoclastic activities in alveolar bone and controlled the OTM process through the MCP-1 secretion and RANKL/OPG system. FAU - Liu, F AU - Liu F AD - 1 Department of Orthodontics, Peking University School and Hospital of Stomatology, Haidian District, Beijing, P.R. China. AD - 2 Center for Craniofacial Stem Cell Research and Regeneration, Peking University School and Hospital of Stomatology, Haidian District, Beijing, P.R. China. FAU - Wen, F AU - Wen F AD - 1 Department of Orthodontics, Peking University School and Hospital of Stomatology, Haidian District, Beijing, P.R. China. FAU - He, D AU - He D AD - 1 Department of Orthodontics, Peking University School and Hospital of Stomatology, Haidian District, Beijing, P.R. China. AD - 2 Center for Craniofacial Stem Cell Research and Regeneration, Peking University School and Hospital of Stomatology, Haidian District, Beijing, P.R. China. FAU - Liu, D AU - Liu D AD - 1 Department of Orthodontics, Peking University School and Hospital of Stomatology, Haidian District, Beijing, P.R. China. AD - 2 Center for Craniofacial Stem Cell Research and Regeneration, Peking University School and Hospital of Stomatology, Haidian District, Beijing, P.R. China. FAU - Yang, R AU - Yang R AD - 1 Department of Orthodontics, Peking University School and Hospital of Stomatology, Haidian District, Beijing, P.R. China. AD - 2 Center for Craniofacial Stem Cell Research and Regeneration, Peking University School and Hospital of Stomatology, Haidian District, Beijing, P.R. China. FAU - Wang, X AU - Wang X AD - 1 Department of Orthodontics, Peking University School and Hospital of Stomatology, Haidian District, Beijing, P.R. China. AD - 2 Center for Craniofacial Stem Cell Research and Regeneration, Peking University School and Hospital of Stomatology, Haidian District, Beijing, P.R. China. FAU - Yan, Y AU - Yan Y AD - 1 Department of Orthodontics, Peking University School and Hospital of Stomatology, Haidian District, Beijing, P.R. China. AD - 2 Center for Craniofacial Stem Cell Research and Regeneration, Peking University School and Hospital of Stomatology, Haidian District, Beijing, P.R. China. FAU - Liu, Y AU - Liu Y AD - 1 Department of Orthodontics, Peking University School and Hospital of Stomatology, Haidian District, Beijing, P.R. China. AD - 2 Center for Craniofacial Stem Cell Research and Regeneration, Peking University School and Hospital of Stomatology, Haidian District, Beijing, P.R. China. FAU - Kou, X AU - Kou X AD - 1 Department of Orthodontics, Peking University School and Hospital of Stomatology, Haidian District, Beijing, P.R. China. AD - 2 Center for Craniofacial Stem Cell Research and Regeneration, Peking University School and Hospital of Stomatology, Haidian District, Beijing, P.R. China. FAU - Zhou, Y AU - Zhou Y AD - 1 Department of Orthodontics, Peking University School and Hospital of Stomatology, Haidian District, Beijing, P.R. China. AD - 2 Center for Craniofacial Stem Cell Research and Regeneration, Peking University School and Hospital of Stomatology, Haidian District, Beijing, P.R. China. LA - eng PT - Journal Article DEP - 20170206 PL - United States TA - J Dent Res JT - Journal of dental research JID - 0354343 RN - 0 (Ccl2 protein, mouse) RN - 0 (Chemokine CCL2) RN - 0 (Osteoprotegerin) RN - 0 (RANK Ligand) RN - YY9FVM7NSN (Hydrogen Sulfide) SB - IM MH - Animals MH - Bone Remodeling/*drug effects MH - Cell Differentiation MH - Cell Movement MH - Chemokine CCL2/metabolism MH - Hydrogen Sulfide/*pharmacology MH - Male MH - Mesenchymal Stem Cells/*cytology MH - Mice MH - Mice, Inbred C57BL MH - Osteoclasts/*cytology MH - Osteoprotegerin/metabolism MH - Periodontal Ligament/*cytology MH - RANK Ligand/metabolism MH - *Tooth Movement Techniques OTO - NOTNLM OT - bone remodeling OT - hydrogen sulfide OT - mechanical force OT - mesenchymal stem cells OT - orthodontic tooth movement OT - osteoclast EDAT- 2017/02/07 06:00 MHDA- 2017/08/09 06:00 CRDT- 2017/02/07 06:00 PHST- 2017/02/07 06:00 [pubmed] PHST- 2017/08/09 06:00 [medline] PHST- 2017/02/07 06:00 [entrez] AID - 10.1177/0022034517690388 [doi] PST - ppublish SO - J Dent Res. 2017 Jun;96(6):694-702. doi: 10.1177/0022034517690388. Epub 2017 Feb 6.