PMID- 28167212 OWN - NLM STAT- MEDLINE DCOM- 20171006 LR - 20211204 IS - 0167-4889 (Print) IS - 0167-4889 (Linking) VI - 1864 IP - 4 DP - 2017 Apr TI - Recruitment of RNA molecules by connexin RNA-binding motifs: Implication in RNA and DNA transport through microvesicles and exosomes. PG - 728-736 LID - S0167-4889(17)30026-5 [pii] LID - 10.1016/j.bbamcr.2017.02.001 [doi] AB - Connexins (Cxs) are integral membrane proteins that form high-conductance plasma membrane channels, allowing communication from cell to cell (via gap junctions) and from cells to the extracellular environment (via hemichannels). Initially described for their role in joining excitable cells (nerve and muscle), gap junctions (GJs) are found between virtually all cells in solid tissues and are essential for functional coordination by enabling the direct transfer of small signalling molecules, metabolites, ions, and electrical signals from cell to cell. Several studies have revealed diverse channel-independent functions of Cxs, which include the control of cell growth and tumourigenicity. Connexin43 (Cx43) is the most widespread Cx in the human body. The myriad roles of Cx43 and its implication in the development of disorders such as cancer, inflammation, osteoarthritis and Alzheimer's disease have given rise to many novel questions. Several RNA- and DNA-binding motifs were predicted in the Cx43 and Cx26 sequences using different computational methods. This review provides insights into new, ground-breaking functions of Cxs, highlighting important areas for future work such as transfer of genetic information through extracellular vesicles. We discuss the implication of potential RNA- and DNA-binding domains in the Cx43 and Cx26 sequences in the cellular communication and control of signalling pathways. CI - Copyright (c) 2017 The Author(s). Published by Elsevier B.V. All rights reserved. FAU - Varela-Eirin, Marta AU - Varela-Eirin M AD - CellCOM research group, Instituto de Investigacion Biomedica de A Coruna (INIBIC), CH-Universitario A Coruna (XXIAC), Servizo Galego de Saude (SERGAS), University of A Coruna, Xubias de Arriba, 84 15006 A Coruna, Spain. FAU - Varela-Vazquez, Adrian AU - Varela-Vazquez A AD - CellCOM research group, Instituto de Investigacion Biomedica de A Coruna (INIBIC), CH-Universitario A Coruna (XXIAC), Servizo Galego de Saude (SERGAS), University of A Coruna, Xubias de Arriba, 84 15006 A Coruna, Spain. FAU - Rodriguez-Candela Mateos, Marina AU - Rodriguez-Candela Mateos M AD - CellCOM research group, Instituto de Investigacion Biomedica de A Coruna (INIBIC), CH-Universitario A Coruna (XXIAC), Servizo Galego de Saude (SERGAS), University of A Coruna, Xubias de Arriba, 84 15006 A Coruna, Spain. FAU - Vila-Sanjurjo, Anton AU - Vila-Sanjurjo A AD - Grupo GIBE, Departamento de Bioloxia Celular e Molecular, Facultade de Ciencias, Universidade de A Coruna (UDC), Campus Zapateira, s/n 15.071, A Coruna, Spain. FAU - Fonseca, Eduardo AU - Fonseca E AD - CellCOM research group, Instituto de Investigacion Biomedica de A Coruna (INIBIC), CH-Universitario A Coruna (XXIAC), Servizo Galego de Saude (SERGAS), University of A Coruna, Xubias de Arriba, 84 15006 A Coruna, Spain. FAU - Mascarenas, Jose L AU - Mascarenas JL AD - Centro Singular de Investigacion en Quimica Bioloxica e Materiais Moleculares (CIQUS), Universidade de Santiago de Compostela, 15782, Santiago de Compostela, Spain; Departamento de Quimica Organica, Universidade de Santiago de Compostela, 15782, Santiago de Compostela, Spain; Departamento de Quimica Inorganica, Universidade de Santiago de Compostela, 15782, Santiago de Compostela, Spain. FAU - Eugenio Vazquez, M AU - Eugenio Vazquez M AD - Centro Singular de Investigacion en Quimica Bioloxica e Materiais Moleculares (CIQUS), Universidade de Santiago de Compostela, 15782, Santiago de Compostela, Spain; Departamento de Quimica Organica, Universidade de Santiago de Compostela, 15782, Santiago de Compostela, Spain; Departamento de Quimica Inorganica, Universidade de Santiago de Compostela, 15782, Santiago de Compostela, Spain. FAU - Mayan, Maria D AU - Mayan MD AD - CellCOM research group, Instituto de Investigacion Biomedica de A Coruna (INIBIC), CH-Universitario A Coruna (XXIAC), Servizo Galego de Saude (SERGAS), University of A Coruna, Xubias de Arriba, 84 15006 A Coruna, Spain. Electronic address: Ma.Dolores.Mayan.Santos@sergas.es. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Review DEP - 20170204 PL - Netherlands TA - Biochim Biophys Acta Mol Cell Res JT - Biochimica et biophysica acta. Molecular cell research JID - 101731731 RN - 0 (Connexin 43) RN - 0 (Connexins) RN - 0 (GJB2 protein, human) RN - 127120-53-0 (Connexin 26) RN - 63231-63-0 (RNA) SB - IM MH - Alzheimer Disease/genetics/metabolism/pathology MH - Animals MH - Biological Transport MH - Cell Communication MH - Cell Proliferation MH - Cell Transformation, Neoplastic/genetics/metabolism/pathology MH - Cell-Derived Microparticles/*metabolism MH - Connexin 26 MH - Connexin 43/genetics/*metabolism MH - Connexins/genetics/*metabolism MH - Exosomes/*metabolism MH - Gap Junctions MH - Humans MH - Inflammation MH - Neoplasms/genetics/metabolism/pathology MH - Osteoarthritis/genetics/metabolism/pathology MH - *Protein Interaction Domains and Motifs MH - RNA/genetics/metabolism OTO - NOTNLM OT - Cellular communication OT - Connexin26 OT - Connexin43 OT - Exosomes OT - Microvesicles OT - RNA-binding domains EDAT- 2017/02/09 06:00 MHDA- 2017/10/07 06:00 CRDT- 2017/02/08 06:00 PHST- 2016/11/09 00:00 [received] PHST- 2017/01/26 00:00 [revised] PHST- 2017/02/02 00:00 [accepted] PHST- 2017/02/09 06:00 [pubmed] PHST- 2017/10/07 06:00 [medline] PHST- 2017/02/08 06:00 [entrez] AID - S0167-4889(17)30026-5 [pii] AID - 10.1016/j.bbamcr.2017.02.001 [doi] PST - ppublish SO - Biochim Biophys Acta Mol Cell Res. 2017 Apr;1864(4):728-736. doi: 10.1016/j.bbamcr.2017.02.001. Epub 2017 Feb 4.