PMID- 28168001
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1749-8546 (Print)
IS  - 1749-8546 (Electronic)
IS  - 1749-8546 (Linking)
VI  - 12
DP  - 2017
TI  - The neuroprotective effects of Tao-Ren-Cheng-Qi Tang against embolic stroke in 
      rats.
PG  - 7
LID - 10.1186/s13020-017-0128-y [doi]
LID - 7
AB  - BACKGROUND: Combinations of the traditional Chinese and Western medicines have 
      been used to treat numerous diseases throughout the world, and there is a growing 
      body of evidence showing that some of the herbs used in traditional Chinese 
      medicine elicit significant pharmacological effects. The aim of this study was to 
      demonstrate the neuroprotective effects of Tao-Ren-Cheng-Qi Tang (TRCQT) in 
      combination with aspirin following middle cerebral artery occlusion 
      (MCAO)-induced embolic stroke in rats. METHODS: A blood clot was embolized into 
      the middle cerebral artery of rats to induce focal ischemic brain injury. After 
      24 h of MCAO occlusion, the rats were arbitrarily separated into five groups and 
      subjected to different oral treatment processes with TRCQT and aspirin for 
      30 days before being evaluated in terms of their neurological behavior using a 
      four-point system. The rats were sacrificed at 30 days after drug treatment and 
      the infarct volumes were measured using a 2,3,5-triphenyltetrazolium chloride 
      staining method. Tumor necrosis factor-alpha (TNF-alpha), c-Jun N-terminal kinases (JNK), 
      activated caspase-3 and Bax were detected by western blot analysis. The apoptotic 
      cells were identified by Terminal deoxynucleotidyl transferase dUTP nick end 
      labeling (TUNEL) staining. ROS generation was also measured by electron spin 
      resonance spectrometry. RESULTS: Rats treated with TRCQT alone or in combination 
      with aspirin showed a significantly reduced infarct volume (P < 0.001) and 
      improved neurological outcome compared with those treated with distilled water. 
      Rats treated with TRCQT alone (P = 0.021) or in combination with aspirin 
      (P = 0.02) also showed significantly reduced MCAO-induced expression levels of 
      TNF-alpha and pJNK (P < 0.001) in their ischemic regions. Rats treated with TRCQT 
      alone or in combination with aspirin showed decreased apoptosis by a reduction in 
      the number of TUNEL positive cells, which inhibited the expression of activated 
      caspase-3 (P = 0.038) and Bax (P = 0.004; P = 0.003). TRCQT also led to a 
      significant concentration-dependent reduction in the formation of hydroxyl 
      radicals (P < 0.001). CONCLUSIONS: TRCQT reduced brain infarct volume and 
      improved neurological outcomes by reducing apoptosis, attenuating the expression 
      of TNF-alpha and p-JNK, and reducing the formation of hydroxyl radicals in 
      MCAO-induced embolic stroke of rats.
FAU - Hsu, Ling-Wei
AU  - Hsu LW
AD  - Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical 
      University, Taipei, Taiwan. ISNI: 0000 0000 9337 0481. GRID: grid.412896.0
FAU - Shiao, Wei-Cheng
AU  - Shiao WC
AD  - Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical 
      University, Taipei, Taiwan. ISNI: 0000 0000 9337 0481. GRID: grid.412896.0
AD  - Department of Internal Medicine, Yuan's General Hospital, Kaohsiung, Taiwan. 
      ISNI: 0000 0004 0622 9252. GRID: grid.417380.9
FAU - Chang, Nen-Chung
AU  - Chang NC
AD  - Department of Internal Medicine, School of Medicine, Taipei Medical University, 
      Taipei, Taiwan. ISNI: 0000 0000 9337 0481. GRID: grid.412896.0
FAU - Yu, Meng-Che
AU  - Yu MC
AD  - Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical 
      University, Taipei, Taiwan. ISNI: 0000 0000 9337 0481. GRID: grid.412896.0
FAU - Yen, Ting-Lin
AU  - Yen TL
AD  - Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical 
      University, Taipei, Taiwan. ISNI: 0000 0000 9337 0481. GRID: grid.412896.0
FAU - Thomas, Philip Aloysius
AU  - Thomas PA
AD  - Department of Microbiology, Institute of Ophthalmology, Joseph Eye Hospital, 
      Tiruchirappalli, Tamil Nadu 620 001 India. GRID: grid.414760.0
FAU - Jayakumar, Thanasekaran
AU  - Jayakumar T
AD  - Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical 
      University, Taipei, Taiwan. ISNI: 0000 0000 9337 0481. GRID: grid.412896.0
FAU - Sheu, Joen-Rong
AU  - Sheu JR
AD  - Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical 
      University, Taipei, Taiwan. ISNI: 0000 0000 9337 0481. GRID: grid.412896.0
AD  - Department of Pharmacology, School of Medicine, Taipei Medical University, 
      Taipei, Taiwan. ISNI: 0000 0000 9337 0481. GRID: grid.412896.0
LA  - eng
PT  - Journal Article
DEP - 20170131
PL  - England
TA  - Chin Med
JT  - Chinese medicine
JID - 101265109
PMC - PMC5286857
EDAT- 2017/02/09 06:00
MHDA- 2017/02/09 06:01
PMCR- 2017/01/31
CRDT- 2017/02/08 06:00
PHST- 2015/01/16 00:00 [received]
PHST- 2017/01/13 00:00 [accepted]
PHST- 2017/02/08 06:00 [entrez]
PHST- 2017/02/09 06:00 [pubmed]
PHST- 2017/02/09 06:01 [medline]
PHST- 2017/01/31 00:00 [pmc-release]
AID - 128 [pii]
AID - 10.1186/s13020-017-0128-y [doi]
PST - epublish
SO  - Chin Med. 2017 Jan 31;12:7. doi: 10.1186/s13020-017-0128-y. eCollection 2017.