PMID- 28168411 OWN - NLM STAT- MEDLINE DCOM- 20180319 LR - 20240418 IS - 1433-3023 (Electronic) IS - 0937-3462 (Print) IS - 0937-3462 (Linking) VI - 28 IP - 6 DP - 2017 Jun TI - Potential therapeutic role of punicalagin against mechanical-trauma-induced stress urinary incontinence via upregulation of Nrf2 and TGF-beta1 signaling : Effect of punicalagin on mechanical trauma induced SUI. PG - 947-955 LID - 10.1007/s00192-017-3283-x [doi] AB - INTRODUCTION AND HYPOTHESIS: We investigated the effect of punicalagin (PUN; 2,3-hexahydroxydiphenoyl-gallagyl-D-glucose), on mechanical-trauma-induced stress urinary incontinence (SUI) in mouse and the mechanisms underlying any effects. METHODS: Ninety virgin female C57BL/6 mice were randomized into six groups: five groups underwent vaginal distention (VD) for 1 h and leak-point pressure (LPP) was measured on the 1st, 3rd, 7th, 14th, and 28th day following (VD groups 1 d, 3 d, 7 d, 14 d, and 28 d). The sixth group was a noninstrumented control (NC) group. Then, 75 virgin female C57BL/6 mice were randomized into five groups: a VD group (that just underwent VD) and an NC group were orally administered saline every day for 7 days; and three VD + PUN groups that underwent VD and were orally administered PUN respectively at 2.5, 5, and 10 mg/kg every day for 7 days. LPP was tested on the day 7, then all mice were sacrificed and their urethras and anterior vaginal walls harvested for Masson staining, immunohistochemistry study, Western blot analysis, and quantitative polymerase chain reaction (qPCR). RESULTS: LPPs after VD were significantly lower than the NC group, and the LPPs of mice on days 14 and 28 day after VD were significantly higher than on the days 1, 3, and 7. PUN significantly improved VD-induced drops in LPP and alleviated VD-induced decrease of collagen I, collagen III, alpha-smooth muscle actin (SMA), transforming growth factor (TGF)-beta1, and p-Smad3, nuclear factor-erythroid 2 p45-related factor 2 (Nrf2), and glutathione peroxidase (GPx1) protein levels, and increase of 8-hydroxydeoxyguanosine (OHdG) in urethra and anterior vaginal wall. PUN also up-regulated the expression of manganese superoxide dismutase (MnSOD), whereas protein levels of Smad 2, p-Smad2, and Smad3 were not changed. CONCLUSIONS: PUN exerts certain therapeutic effect on mechanical-trauma-induced SUI in mice, which might be through the activation of TGF-beta1/Smad3 and Nrf2/antioxidant response element (ARE) signaling activation. FAU - Tang, Jianming AU - Tang J AD - Department of Gynecology and Obstetrics, Renmin Hospital of Wuhan University, #238 Liberation Road, Wuhan, 430060, Hubei Province, People's Republic of China. FAU - Liu, Cheng AU - Liu C AD - Department of Gynecology and Obstetrics, Renmin Hospital of Wuhan University, #238 Liberation Road, Wuhan, 430060, Hubei Province, People's Republic of China. FAU - Min, Jie AU - Min J AD - Department of Gynecology and Obstetrics, Renmin Hospital of Wuhan University, #238 Liberation Road, Wuhan, 430060, Hubei Province, People's Republic of China. FAU - Hu, Ming AU - Hu M AD - Department of Gynecology and Obstetrics, Renmin Hospital of Wuhan University, #238 Liberation Road, Wuhan, 430060, Hubei Province, People's Republic of China. FAU - Li, Yang AU - Li Y AD - Department of Gynecology and Obstetrics, Renmin Hospital of Wuhan University, #238 Liberation Road, Wuhan, 430060, Hubei Province, People's Republic of China. FAU - Hong, Li AU - Hong L AD - Department of Gynecology and Obstetrics, Renmin Hospital of Wuhan University, #238 Liberation Road, Wuhan, 430060, Hubei Province, People's Republic of China. drhongli7777@gmail.com. LA - eng PT - Journal Article DEP - 20170206 PL - England TA - Int Urogynecol J JT - International urogynecology journal JID - 101567041 RN - 0 (Actins) RN - 0 (Hydrolyzable Tannins) RN - 0 (NF-E2-Related Factor 2) RN - 0 (Nfe2l2 protein, mouse) RN - 0 (Smad3 Protein) RN - 0 (Transforming Growth Factor beta1) RN - 0 (alpha-smooth muscle actin, mouse) RN - 65995-63-3 (punicalagin) RN - 88847-89-6 (8-Hydroxy-2'-Deoxyguanosine) RN - 9007-34-5 (Collagen) RN - EC 1.11.1.9 (Glutathione Peroxidase) RN - EC 1.15.1.1 (Superoxide Dismutase) RN - G9481N71RO (Deoxyguanosine) RN - EC 1.11.1.9 (Glutathione Peroxidase GPX1) RN - EC 1.11.1.9 (Gpx1 protein, mouse) SB - IM EIN - Int Urogynecol J. 2024 Apr 16;:. PMID: 38625605 MH - 8-Hydroxy-2'-Deoxyguanosine MH - Actins/metabolism MH - Animals MH - Collagen/metabolism MH - Deoxyguanosine/analogs & derivatives/metabolism MH - Disease Models, Animal MH - Female MH - Glutathione Peroxidase/metabolism MH - Hydrolyzable Tannins/*pharmacology MH - Mice MH - Mice, Inbred C57BL MH - NF-E2-Related Factor 2/*metabolism MH - Smad3 Protein/metabolism MH - Superoxide Dismutase/metabolism MH - Transforming Growth Factor beta1/*metabolism MH - Up-Regulation/*drug effects MH - Urethra/metabolism MH - Urinary Incontinence, Stress/*drug therapy/etiology/metabolism MH - Vagina/metabolism MH - Glutathione Peroxidase GPX1 PMC - PMC5437194 OTO - NOTNLM OT - Extracellular matrix OT - Mechanical trauma OT - Oxidative damage OT - Punicalagin OT - Stress urinary incontinence OT - Vaginal distension COIS- None. EDAT- 2017/02/09 06:00 MHDA- 2018/03/20 06:00 PMCR- 2017/02/06 CRDT- 2017/02/08 06:00 PHST- 2016/11/19 00:00 [received] PHST- 2017/01/19 00:00 [accepted] PHST- 2017/02/09 06:00 [pubmed] PHST- 2018/03/20 06:00 [medline] PHST- 2017/02/08 06:00 [entrez] PHST- 2017/02/06 00:00 [pmc-release] AID - 10.1007/s00192-017-3283-x [pii] AID - 3283 [pii] AID - 10.1007/s00192-017-3283-x [doi] PST - ppublish SO - Int Urogynecol J. 2017 Jun;28(6):947-955. doi: 10.1007/s00192-017-3283-x. Epub 2017 Feb 6.